关键词:水稻; ospls3; 叶片早衰; 生理分析; 基因定位 Physiological Characteristics and Gene Mapping of a Precocious Leaf Senescence Mutant ospls3 in Rice GONG Pan1,**, LI Kun-Yu1,**, HUANG Fu-Deng2, WEI Li-Quan1, YANG Xi1, CHENG Fang-Min1, PAN Gang1,* 1 College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China
2 Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China
Fund:This study was supported by the National Natural Science Foundation of China (31271691) and the Major Project of China on New Varieties of GMO Cultivation (2013ZX08001-002) AbstractLeaf senescence is the final stage of leaf development. However, premature aging of functional leaves leads to yield reduction and quality decline. Thus, it is very important for developing novel crop germplasms with delayed leaf-senescence characteristics through investigating the molecular mechanism of leaf senescence. In this study, an ospls3 ( Oryza sativa precocious leaf senescence 3) mutant, produced by60Co γ-radiation treatment of indica cultivar N142, was identified. The symptoms of the premature senescence mutant presented firstly at tillering stage showing brown leaf tip and brown spots in top part of leaf blade, then spread rapidly to basal part of leaf blade and led leaf to die. The physiological analysis indicated that, in the ospls3 mutant, the content of chlorophyll was the highest in the flag leaf, the following was in second-top and third-top leaves, but all of them were significantly lower than those in the wild type. The contents of MDA, O2?, and H2O2 and the activities of SOD and POD among the top three leaves in the wild type maintained similar levels, which were significantly lower than those in the mutant. The soluble protein contents and the activity of CAT had no significant difference among top three leaves in the wild type while significantly decreased in the mutant. Genetic analysis verified that the ospls3 is a recessive mutant and was mapped in a 294 kb interval between RM6953 and RM28753 on the long arm of chromosome 12, which establishes a solid foundation for further cloning and functional studies of this gene.
Keyword:Rice; ospls3; Precocious leaf senescence; Physiological analysis; Gene mapping Show Figures Show Figures
图1 不同生育期突变体ospls3及其野生型的表型A: 分蘖期; B: 抽穗期; C: 灌浆结实期; D: 抽穗期叶片(1~4代表剑叶至倒四叶); Bar = 20 cm。Fig. 1 Phenotype of ospls3 and its wild-type (WT) plants at different growth stages A: tillering stage; B: heading stage; C: filling stage; D: leaves at heading stage (1-4 means the flag leaf to 4th leaf from top); Bar = 20 cm.
表1 Table 1 表1(Table 1)
表1 突变体ospls3及其野生型的农艺性状 Table 1 Agronomic traits of ospls3 and its wild-type (WT) plants
性状 Trait
2013
2014
野生型WT
突变体ospls3
野生型WT
突变体ospls3
株高 Plant height (cm)
112.32± 2.83
81.19± 2.56* *
109.78± 2.78
79.31± 3.45* *
有效穗数 Effective panicle number
11.52± 2.43
7.34± 1.25* *
12.74± 2.79
9.15± 0.84* *
每穗粒数 Grain number per panicle
175.79± 24.84
47.71± 9.17* *
167.43± 25.94
58.42± 12.65* *
结实率 Seed-setting rate (%)
88.05± 3.92
28.85± 2.84* *
80.34± 5.35
19.39± 4.11* *
千粒重 1000-grain weight (g)
25.33± 0.79
16.65± 1.13* *
24.68± 0.74
15.34± 1.12* *
单株产量 Yield per plant (g)
47.27± 5.32
2.78± 0.56* *
44.35± 7.72
1.69± 0.42* *
* * 表示在0.01水平上差异显著。* * Significant difference at P< 0.01.
表1 突变体ospls3及其野生型的农艺性状 Table 1 Agronomic traits of ospls3 and its wild-type (WT) plants
图3 孕穗期突变体ospls3及其野生型叶片的O2?、H2O2、MDA和可溶性蛋白含量1: 剑叶; 2: 倒二叶; 3: 倒三叶。* 表示在0.05水平上差异显著; * * 在0.01水平上差异显著。Fig. 3 O2?, H2O2, MDA, and soluble protein contents of ospls3 and its wild-type (WT) plants at booting stage1: flag leaves; 2: 2nd leaves from top; 3: 3rd leaves from top. * Significant difference at P< 0.05; * * Significant difference at P< 0.01.
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