关键词:小麦; 蛋白质含量; 关联分析; 分子标记 Genetic Diversity and Association Analysis of Protein Content in Weak Gluten Wheat Ningmai 9 and Its Derived Lines JIANG Peng, ZHANG Ping-Ping, ZHANG Xu, CHEN Xiao-Lin, YAO Jin-Bao, MA Hong-Xiang* Jiangsu Provincial Key Laboratory for Agrobiology / Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China Fund:This study was supported by the Modern Agro-industry Technology System (CARS-3), the Independent Innovation of Agricultural Sciences in Jiangsu Province (CX14-2002), the Key Technology R&D Program of Jiangsu Province (BE2013439), and the Program of Introducing International Super Agricultural Science and Technology (948 Program) [2011-G3(2)] AbstractNingmai 9 is an elite weak gluten wheat cultivar and an important breeding parent in the southern area of Huai River Valley. To date, 15 new cultivars have been developed and released from Ningmai 9. This study aimed at dissecting the genetic mechanism and inheritance of protein content in Ningmai 9 and its derivatives. The kernel protein content (KPC) and flour protein content (FPC) of Ningmai 9 and its 117 derivatives were evaluated in the 2009-2010 and 2010-2011 growing seasons and the QTLs associated with KPC and FPC were identified by whole genomes screening with 185 SSR markers. The genetic similarity index ranged from 0.55 to 0.88 among Ningmai 9 and its derivatives. In the Neighbor-Joining cluster tree, Ningmai 9 was clustered with most first-generation lines first, then with the second-generation lines, and Yangfumai 4 of first-generation derivative was the last one to incorporate in. The first- and second-generation derivatives inherited 75.60% and 67.81% of Ningmai 9 alleles, respectively. Great variations of KPC and FPC were observed in the Ningmai 9 derived lines with the variation coefficients ranging from 5.07% to 7.28%. Six and five QTLs were identified to be associated with KPC and FPC, of which three and two were stably detected in both years, respectively. One QTL was associated with both KPC and FPC. Three stable QTLs, Xgwm539, Xwmc397, and Xwmc468, had negative effects on KPC or FPC in Ningmai 9 and might be used for quality improvement in weak-gluten wheat breeding.
Keyword:Wheat; Protein content; Association analysis; Molecular markers Show Figures Show Figures
图1 宁麦9号及其衍生品种(系)的Neighbor-Joining遗传聚类分析品种名称详见附表。L7遗传距离较大, 以虚线表示。Fig. 1 Dendrogram of Ningmai 9 and its derivatives based on Neighbor-Joining analysis Variety names are listed in supplementary table. The genetic distance of L7 is very large and broken down with dashed line.
表4 籽粒蛋白质含量和面粉蛋白质含量关联位点在宁麦9号衍生世代中的分布频率 Table 4 Frequencies of markers associated with kernel protein content and flour protein content in Ningmai 9 derived generations (%)
世代 Generation
Xgwm539
Xgwm508
Xwmc397
Xwmc468
衍生一代 First generation
54.05
68.42
94.87
92.31
衍生二代 Second generation
38.46
47.22
88.46
71.79
全部世代 Combined
43.48
54.55
91.45
79.49
表4 籽粒蛋白质含量和面粉蛋白质含量关联位点在宁麦9号衍生世代中的分布频率 Table 4 Frequencies of markers associated with kernel protein content and flour protein content in Ningmai 9 derived generations (%)
附表 Table 附表(Table)
附表 供试宁麦衍生品种(系)及其编号 Table Supplementary table Varieties (lines) derived from Ningmai 9 and their codes
世代 Generation
编号 No.
品种(系) Variety (line)
编号 No.
品种(系) Variety (line)
编号 No.
品种(系) Variety (line)
亲本 Parent
L1
宁麦9号 Ningmai 9
衍生一代
L2
宁麦13 Ningmai 13
L15
宁0866 Ning 0866
L28
宁0327 Ning 0327
First generation
L3
宁麦14 Ningmai 14
L16
宁0894 Ning 0894
L29
宁0331 Ning 0331
L4
宁麦16 Ningmai 16
L17
宁08105 Ning 08105
L35
宁9-11 Ning 9-11
L5
生选6号 Shengxuan 6
L18
宁0561 Ning 0561
L36
宁9-36 Ning 9-36
L6
扬麦18 Yangmai 18
L19
宁0564 Ning 0564
L37
宁9大41 Ning 9 Da 41
L7
扬幅麦4号Yangfumai 4
L20
宁0565 Ning 0565
L38
宁9大44 Ning 9 Da 44
L8
3E/158
L21
宁0417 Ning 0417
L39
宁9大76 Ning 9 Da 76
L9
南农0686 Nannong 0686
L22
宁0418 Ning 0418
L40
宁9大78 Ning 9 Da 78
L10
宁麦18 Ningmai 18
L23
宁0422 Ning 0422
L41
宁9大80 Ning 9 Da 80
L11
宁0556 Ning 0556
L24
宁0311 Ning 0311
L60
71666
L12
宁07123 Ning 07123
L25
宁0316 Ning 0316
L61
6E/123
L13
宁07119 Ning 07119
L26
宁0319 Ning 0319
L62
09-654
L14
宁0853 Ning 0853
L27
宁0320 Ning 0320
L64
09-444
衍生二代
L30
宁0798 Ning 0798
L67
宁0881 Ning 0881
L93
08F423
Second generation
L31
宁07117 Ning 07117
L68
宁0883 Ning 0883
L94
08F424
L32
F307
L69
宁0886 Ning 0886
L95
08F426
L33
F308
L70
宁0896 Ning 0896
L96
08F432
L34
宁0797 Ning 0797
L71
宁08109 Ning 08109
L97
08F433
L42
宁0862 Ning 0862
L72
宁08111 Ning 08111
L98
08F434
L43
宁0869 Ning 0869
L73
宁08112 Ning 08112
L99
08F435
L44
宁0872 Ning 0872
L74
宁08113 Ning 08113
L100
08F436
L45
宁0880 Ning 0880
L75
08F331
L101
08F437
L46
宁0882 Ning 0882
L76
08F333
L102
08F442
L47
宁0884 Ning 0884
L77
08F337
L103
08F443
L48
宁0887 Ning 0887
L78
08F353
L104
08F444
L49
宁0893 Ning 0893
L79
08F362
L105
08F445
L50
宁0895 Ning 0895
L80
08F386
L106
08F446
L51
宁0897 Ning 0897
L81
08F387
L107
08F448
L52
宁0898 Ning 0898
L82
08F396
L108
08F449
L53
宁0899 Ning 0899
L83
08F397
L109
08F450
L54
宁08102 Ning 08102
L84
08F399
L110
08F451
L55
宁08104 Ning 08104
L85
08F406
L111
08F453
L56
宁08108 Ning 08108
L86
08F407
L112
08F454
L57
宁08110 Ning 08110
L87
08F408
L113
08F457
L58
宁08115 Ning 08115
L88
08F409
L114
08F458
L59
宁08116 Ning 08116
L89
08F410
L115
08F468
L63
09-569
L90
08F411
L116
08F459
L65
镇麦166 Zhenmai 166
L91
08F417
L117
08F516
L66
宁0867 Ning 0867
L92
08F418
L118
08F517
附表 供试宁麦衍生品种(系)及其编号 Table Supplementary table Varieties (lines) derived from Ningmai 9 and their codes
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