李倩¹, 王罡¹, 张松皓¹, 杨丹¹, 王昱蓉², 季静¹, 安婷¹, 李辰¹, 马志刚³, 史怀宇¹, 关春峰¹, 刘玉⁴
AuthorsHTML:李倩¹, 王罡¹, 张松皓¹, 杨丹¹, 王昱蓉², 季静¹, 安婷¹, 李辰¹, 马志刚³, 史怀宇¹, 关春峰¹, 刘玉⁴
AuthorsListE:Li Qian¹, Wang Gang ¹, Zhang Songhao¹, Yang Dan¹, Wang Yurong², Ji Jing ¹, An Ting¹, \rLi Chen¹, Ma Zhigang³, Shi Huaiyu¹, Guan Chunfeng¹
AuthorsHTMLE:Li Qian¹, Wang Gang ¹, Zhang Songhao¹, Yang Dan¹, Wang Yurong², Ji Jing ¹, An Ting¹, \rLi Chen¹, Ma Zhigang³, Shi Huaiyu¹, Guan Chunfeng¹
Unit:1. 天津大学环境科学与工程学院，天津 300072；2. 天津市天大天福生物技术有限公司，天津 300072；3. 天津大学化工学院，天津 300072；4. 电子科技大学生命科学与技术学院，成都 610054
Unit_EngLish:1. School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, China
2．Tianjin Tianda Tianfu Bio．Co., Ltd, Tianjin 300072, China
3．School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
4．School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
Abstract_Chinese:氨基酸透性酶(AAP)是植物中的氨基酸转运蛋白, 在氨基酸转运过程中发挥着重要作用．以野生拟南芥(生态型col-0)为材料, 利用PCR 和DNA 重组技术, 克隆得到拟南芥中的氨基酸透性酶基因(AtAAP6). 该基因全长1 446 bp, 构建AtAAP6基因的植物表达载体pCAMBIA2300-AtAAP6, 并利用农杆菌介导的遗传转化方法在马铃薯品种“早大白”中异源过表达AtAAP6基因．经过抗性筛选与植株再生, 成功得到马铃薯转基因植株, 并诱导得到转基因马铃薯微型薯．RT-PCR结果表明, AtAAP6基因在转基因马铃薯中都能正常地转录表达．这一研究为进一步开展AtAAP6基因功能验证, 提高马铃薯块茎中的氨基酸以及贮藏蛋白的含量提供了研究基础．
Abstract_English:The amino acid permease(AAP)is the amino acid transporter in plants and plays an important role in the transport of amino acids. Amino acid permease gene(AtAAP6)was cloned by PCR and recombinant DNA technologies using Arabidopsis thaliana(Columbia ecotype)as material. The full-length of the AtAAP6 gene is 1 446 bp. The plant expression vector pCAMBIA2300-AtAAP6 was constructed and transformed into virus-free plants of potato cultivar \"Zaodabai\" via Agrobacterium tumefaciens-mediated genetic transformation method. After resistance selection and plant regeneration，transgenic potato plants were successfully obtained. Meanwhile，transgenic potato microtubers were obtained successfully. The RT-PCR analysis proves that the AtAAP6 gene could conduct normal transcription and expression in transgenic potato plants. This study provides the basis for further research on the functional verification of AtAAP6 gene and the improvement of amino acids and proteins storage in potato tubers.
Keyword_Chinese:氨基酸透性酶; 马铃薯; AtAAP6 基因; 表达载体; 遗传转化
Keywords_English:amino acid permease; potato; AtAAP6 gene; expression vector; genetic transformation

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