摘要/Abstract
以邻羟基苯乙酮和对甲基苯甲醛为原料,合成了合成了一种3-羟基黄酮酯化物HFBA.通过探针HFBA对N2H4的识别性质研究,结果表明,探针HFBA在二甲亚砜-水溶液[V(DMSO):V(H2O)=7:3,PBS 20 mmol/L,pH=7.4]中对N2H4具有良好的选择性和灵敏度,且响应快速,抗干扰能力强,其检测限为0.11 μmol/L.细胞成像实验表明,探针HFBA可用于细胞内N2H4的检测.
关键词: 黄酮, 荧光, 肼, 生物成像
A 3-hydroxyflavonoid esters HFBA was synthesized by the reaction of 2-hydroxyacetophenone with p-methyl benzaldehyde, and its structure was characterized by NMR and high resolution mass spectrometer. The recognition behaviors of HFBA to hydrazine were investigated and the results show that HFBA exhibits good selectivity and sensitivity to N2H4 with fast response and good anti-interference ability in DMSO/PBS buffer (pH=7.4). Furthermore, the detection limit of HFBA for hydrazine was estimated to be 0.11 μmol/L, and the probe HFBA was successfully applied for the imaging of hydrazine in living cells.
Key words: flavone, fluorescence, hydrazine, biological imaging
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