摘要/Abstract

利用各种柱色谱和高效液相色谱等分离纯化方法，从砂贝母鳞茎中分离得到6个异甾体生物碱类化合物.根据质谱、一维/二维核磁共振谱和X射线单晶衍射等技术鉴定了它们的结构，分别为karelinine（1），5-epikarelinine（2），27-epiebeienine（3），ebeienine（4），persicanidine B（5）和heilonine（6），其中化合物1~3为新化合物.化合物1是贝母属中罕见的5β-jervine型异甾体生物碱，具有A/B环顺式稠合方式.化合物1和2也是贝母属中首次发现的具有15α-OH取代的jervine型生物碱.
关键词: 砂贝母, 异甾体生物碱, karelinine, 5-epikarelinine, 27-epiebeienine
Three new isosteroidal alkaloids, karelinine (1), 5-epikarelinine (2), and 27-epiebeienine (3), were isolated from the bulbs of Fritillaria karelinii by column chromatography and semipreparative HPLC (high performance liquid chro-matography), together with three known ones, ebeienine (4), persicanidine B (5), and heilonine (6). Their structures were identified by MS, 1D/2D NMR, and single-crystal X-ray diffraction. Compound 1 is a 5β-jervine isosteroidal alkaloid featuring a cis-fused A/B ring moiety, rarely found in the Fritillaria genus. Compounds 1 and 2 also represent the first jervine alkaloids with a 15 α-hydroxy group from this genus.
Key words: Fritillaria karelinii, isosteroidal alkaloids, karelinine, 5-epikarelinine, 27-epiebeienine


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