Abstract:In order to understand the effect of ammonia stress on the performance of anaerobic digestion (AD) of swine manure at different temperatures, the semi-continuous anaerobic digestion experiments with swine manure as substrate were carried out at three temperatures. During the experiments, the concentration of ammonia nitrogen was increased by adding exogenous ammonium chloride. The results showed that the specific methane yield (SMY) decreased significantly when the total ammonia nitrogen (TAN) mass concentration reached 4 000 mg·L?1 under different temperatures, and half maximal inhibitory concentration (IC50) of TAN on SMY at 44 ℃ was 6 845 mg·L?1, corresponding to free ammonia nitrogen (FAN) mass concentrations 1 257 mg·L?1. At a high TAN mass concentrations of approximately 7 000 mg·L?1, the 44 ℃ digestion system showed better tolerance. Ammonia stress caused the accumulation of three fatty acids at 35 ℃ and 44 ℃, including propionic acid, butyric acid, and valeric acid. The processes and rates of the formation and decomposition of the three fatty acids at 55 ℃ were significantly different from those at 35 ℃ and 44 ℃. Pearson correlation analysis results showed that the main factor affecting methane yield through ammonia inhibition at 35 ℃ and 44 ℃ was FAN (NH3), and the main factor at 55 ℃ was TAN. The results of this study can provide a reference for the optimization of the anaerobic fermentation process of aquaculture manure. Key words:livestock waste/ anaerobic digestion/ methane production/ ammonia nitrogen inhibition/ digestion temperature.
图1实验装置简图 Figure1.Schematic diagram of experimental device
图5各反应器总有机酸、乙酸、丙酸、丁酸和戊酸的质量浓度变化 Figure5.Mass concentrations of total volatile fatty acids, acetic acid, propionic acid, butyric acid and valeric acid in reactors
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1.Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China 2.Hangzhou Energy and Environmental Engineering Co. Ltd, Hangzhou 310020, China Received Date: 2021-07-12 Accepted Date: 2021-10-05 Available Online: 2021-11-18 Keywords:livestock waste/ anaerobic digestion/ methane production/ ammonia nitrogen inhibition/ digestion temperature Abstract:In order to understand the effect of ammonia stress on the performance of anaerobic digestion (AD) of swine manure at different temperatures, the semi-continuous anaerobic digestion experiments with swine manure as substrate were carried out at three temperatures. During the experiments, the concentration of ammonia nitrogen was increased by adding exogenous ammonium chloride. The results showed that the specific methane yield (SMY) decreased significantly when the total ammonia nitrogen (TAN) mass concentration reached 4 000 mg·L?1 under different temperatures, and half maximal inhibitory concentration (IC50) of TAN on SMY at 44 ℃ was 6 845 mg·L?1, corresponding to free ammonia nitrogen (FAN) mass concentrations 1 257 mg·L?1. At a high TAN mass concentrations of approximately 7 000 mg·L?1, the 44 ℃ digestion system showed better tolerance. Ammonia stress caused the accumulation of three fatty acids at 35 ℃ and 44 ℃, including propionic acid, butyric acid, and valeric acid. The processes and rates of the formation and decomposition of the three fatty acids at 55 ℃ were significantly different from those at 35 ℃ and 44 ℃. Pearson correlation analysis results showed that the main factor affecting methane yield through ammonia inhibition at 35 ℃ and 44 ℃ was FAN (NH3), and the main factor at 55 ℃ was TAN. The results of this study can provide a reference for the optimization of the anaerobic fermentation process of aquaculture manure.
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