Effects of two phosphate growth environments on simultaneous removal and enrichment of phosphate
YOU Xingyi1,2,, FENG Xin1,2, PAN Yang1,2,3,,, HUANG Yong1,2,3, XU Linjian1,2 1.School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China 2.Environment Biotechnology Research Institute, Suzhou University of Science and Technology, Suzhou 215009, China 3.Jiangsu Key Laboratory of Environment Science and Engineering, Suzhou 215009, China
Abstract:The aim of this study was to simultaneously remove and enrich phosphate solutions. The phosphorus removal and release capacity of suspended packed biofilm reactor in low concentration phosphate environment or continuous alternation environment of aerobic low concentration phosphate and anaerobic high concentration phosphate were studied. Sludge samples taken from 0, 45 and 95 d were characterized by SEM and high-throughput sequencing. The results showed that the phosphate concentration of aerobic effluent was below 0.5 mg·L?1 and the maximum phosphorus release was 6.05 mg·L?1 in the low phosphorus environment. In the low phosphorus concentration and high phosphorus concentration alternating environment, most of the phosphate concentration of aerobic effluent was below 0.5 mg·L?1, and the concentration of anaerobic phosphate concentrate was up to 63 mg·L?1. The SEM images showed that the main microorganisms in the suspended packed biofilm reactor for simultaneous removal and enrichment of phosphoric acid were bacilli. The result of high-throughput sequencing showed that the relative abundance of Proteobacteria on 0, 45 and 95 d were 48.3%, 57.1% and 89.1%, respectively, and it was dominant bacteria phylum. The relative abundances of Rhodocyclaceae were 18.1%, 19.0% and 30.8%, respectively, which was the dominant bacteria family in the reactor. Zoogloea was the main functional bacterium. In the suspended packing biofilm process, phosphorus accumulating bacteria cultured in alternate growth environment of low phosphorus and high phosphorus could lead to phosphate content in aerobic effluent which meets the national discharge standard, and could obtain high concentration phosphate enrichment solution in anaerobic phase. This growth environment was more suitable for the growth of phosphorus-accumulating microorganisms. Key words:simultaneous removal and enrichment of phosphate/ microbial growth environment/ biofilm/ high-throughput sequencing.
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1.School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China 2.Environment Biotechnology Research Institute, Suzhou University of Science and Technology, Suzhou 215009, China 3.Jiangsu Key Laboratory of Environment Science and Engineering, Suzhou 215009, China Received Date: 2018-11-30 Accepted Date: 2019-04-23 Available Online: 2019-10-11 Keywords:simultaneous removal and enrichment of phosphate/ microbial growth environment/ biofilm/ high-throughput sequencing Abstract:The aim of this study was to simultaneously remove and enrich phosphate solutions. The phosphorus removal and release capacity of suspended packed biofilm reactor in low concentration phosphate environment or continuous alternation environment of aerobic low concentration phosphate and anaerobic high concentration phosphate were studied. Sludge samples taken from 0, 45 and 95 d were characterized by SEM and high-throughput sequencing. The results showed that the phosphate concentration of aerobic effluent was below 0.5 mg·L?1 and the maximum phosphorus release was 6.05 mg·L?1 in the low phosphorus environment. In the low phosphorus concentration and high phosphorus concentration alternating environment, most of the phosphate concentration of aerobic effluent was below 0.5 mg·L?1, and the concentration of anaerobic phosphate concentrate was up to 63 mg·L?1. The SEM images showed that the main microorganisms in the suspended packed biofilm reactor for simultaneous removal and enrichment of phosphoric acid were bacilli. The result of high-throughput sequencing showed that the relative abundance of Proteobacteria on 0, 45 and 95 d were 48.3%, 57.1% and 89.1%, respectively, and it was dominant bacteria phylum. The relative abundances of Rhodocyclaceae were 18.1%, 19.0% and 30.8%, respectively, which was the dominant bacteria family in the reactor. Zoogloea was the main functional bacterium. In the suspended packing biofilm process, phosphorus accumulating bacteria cultured in alternate growth environment of low phosphorus and high phosphorus could lead to phosphate content in aerobic effluent which meets the national discharge standard, and could obtain high concentration phosphate enrichment solution in anaerobic phase. This growth environment was more suitable for the growth of phosphorus-accumulating microorganisms.
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