尹晓宇,
董文静,
李嘉伟,
齐澈力木格,
于永利,
杨景峰,
董武^,
内蒙古民族大学动物科学技术学院, 内蒙古自治区毒物监控及毒理学重点实验室, 通辽 028000

作者简介: 尹晓宇(1994-),男,硕士研究生,研究方向为毒理学,E-mail:yinxiaoyu344@163.com.

通讯作者: 董武,dongwu@imun.edu.cn

基金项目: 国家自然科学基金资助项目（21567019，81360508）；内蒙古民族大学特色交叉学科群建设项目（MDXK008）；内蒙古自治区自然科学基金资助项目（2018MS08033）；内蒙古自治区高等学校科学研究项目（NJZC17203）；内蒙古草原英才2020年度滚动支持（董武）；内蒙古毒物监测及毒理学重点实验室开放项目（MDK2019074，MDK2019076）；内蒙古民族大学研究生科研创新项目（NMDSS1860）；蒙药研发国家地方联合工程研究中心开放基金资助项目（MDK2019051）


中图分类号: X171.5

BDE-99 from Indoor Dust Interferes with Dio3 and Affects Thyroid Function Regulation in Zebrafish

Yin Xiaoyu,
Dong Wenjing,
Li Jiawei,
Qi Chelimuge,
Yu Yongli,
Yang Jingfeng,
Dong Wu^,
College of Animal Science and Technology, Inner Mongolia University for Nationalities, Inner Mongolia Key Laboratory of Toxicant Monitoring and Toxicology, Tongliao 028000, China

Corresponding author: Dong Wu,dongwu@imun.edu.cn

CLC number: X171.5

-->

摘要
HTML全文
图(0)表(0)
参考文献(28)
相关文章
施引文献
资源附件(0)
访问统计

摘要:多溴联苯醚（PBDEs）是一种溴化阻燃剂，在办公室和家庭的灰尘中均有检出。PBDEs会对内分泌造成干扰，主要影响甲状腺激素的调节功能。使用斑马鱼胚胎为动物模型，评价办公室和家庭灰尘萃取物以及BDE-99对斑马鱼胚胎的影响。结果显示，灰尘萃取物和BDE-99同样导致斑马鱼眼部色素降低，GC-MS检测发现灰尘中含有BDE-99和BDE-47，且BDE-99含量远高于BDE-47，通过评估BDE-99在斑马鱼体内代谢及其对甲状腺激素脱碘酶Ⅲ（Dio3）表达的影响，发现Dio3在斑马鱼胚胎原肾管部位表达，且表达量显著增加，72 hpf斑马鱼胚胎的甲状腺素含量显著降低。研究表明，BDE-99对斑马鱼胚胎早期Dio3的活性有干扰作用，并导致甲状腺素含量降低，由此可知，斑马鱼胚胎色素变化和Dio3 mRNA表达有可能作为PBDEs的生物学评价指标。
关键词: PBDEs/
灰尘/
斑马鱼胚胎/
Dio3/
BDE-99

Abstract:PBDEs are brominated flame retardants that have been detected in dust in offices and homes. PBDEs can cause endocrine disruption by mainly affecting the regulation of thyroid hormones. This study used zebrafish embryos as a test species to evaluate the effects of the extracts of office and household dust and BDE-99 on zebrafish embryos. The results showed that dust extracts and BDE-99 both caused a decrease in the pigmentation of the zebrafish eyes. GC-MS analysis found that BDE-99 and BDE-47 were contained in the dust and the content of BDE-99 was much higher than BDE-47. By evaluating the effects of BDE-99 on zebrafish metabolism and the expression of the thyroid hormone deiodinase Ⅲ (Dio3), it was found that the expression of Dio3 was significantly enhanced in the protorenal duct of the zebrafish embryo. The thyroid hormone content in the 72 hpf zebrafish embryo was significantly reduced. The above results show that BDE-99 interferes with the activity of Dio3 in zebrafish embryos and leads to a decrease in thyroxine content. This study indicates that alterations in pigment of the zebrafish embryo and Dio3 mRNA expression may be used as biological indicators of PBDEs.
Key words:PBDEs/
dust/
zebrafish embryos/
Dio3/
BDE-99.

Zezza D, Tait S, Della Salda L, et al. Toxicological, gene expression and histopathological evaluations of environmentally realistic concentrations of polybrominated diphenyl ethers PBDE-47, PBDE-99 and PBDE-209 on zebrafish embryos[J]. Ecotoxicology and Environmental Safety, 2019, 183:109566

Giraudo M, Douville M, Letcher R J, et al. Effects of food-borne exposure of juvenile rainbow trout (Oncorhynchus mykiss) to emerging brominated flame retardants 1,2-bis(2,4,6-tribromophenoxy)ethane and 2-ethylhexyl-2,3,4,5-tetrabromobenzoate[J]. Aquatic Toxicology, 2017, 186:40-49

Johnson-Restrepo B, Kannan K. An assessment of sources and pathways of human exposure to polybrominated diphenyl ethers in the United States[J]. Chemosphere, 2009, 76(4):542-548

Smielowska M, Zabiegala B. Matrix solid-phase dispersion (MSPD) as simple and useful sample preparation technique for determination of polybrominated diphenyl ethers (PBDEs) in dust[J]. Analytica Chimica Acta, 2019, 1084:33-42

Bu Z, Xu X, Xu Q, et al. Indoor polybrominated diphenyl ethers in urban China:An exposure and risk assessment based on settled dust from selected urban regions[J]. Science of the Total Environment, 2020, 714:136808

Zhu N Z, Liu L Y, Ma W L, et al. Polybrominated diphenyl ethers (PBDEs) in the indoor dust in China:Levels, spatial distribution and human exposure[J]. Ecotoxicology and Environmental Safety, 2015, 111:1-8

Babayemi J O, Osibanjo O, Sindiku O, et al. Inventory and substance flow analysis of polybrominated diphenyl ethers in the Nigerian transport sector-end-of-life vehicles policy and management[J]. Science of the Total Environment, 2018, 25(32):31805-31818

Huwe J K, Hakk H, Smith D J, et al. Comparative absorption and bioaccumulation of polybrominated diphenyl ethers following ingestion via dust and oil in male rats[J]. Environmental Science & Technology, 2008, 42(7):2694-2700

Watkins D J, McClean M D, Fraser A J, et al. Associations between PBDEs in office air, dust, and surface wipes[J]. Environment International, 2013, 59:124-132

Zhao X, Wang H, Li J, et al. The correlation between polybrominated diphenyl ethers (PBDEs) and thyroid hormones in the general population:A meta-analysis[J]. PLoS One, 2015, 10(5):e0126989

Arkoosh M R, Van Gaest A L, Strickland S A, et al. Alteration of thyroid hormone concentrations in juvenile Chinook salmon (Oncorhynchus tshawytscha) exposed to polybrominated diphenyl ethers, BDE-47 and BDE-99[J]. Chemosphere, 2017, 171:1-8

Qian B, Wang C, Zhao C, et al. Effects of maternal exposure to BDE209 on neuronal development and transcription of iodothyronine deiodinase in offspring mice[J]. Toxicology Mechanisms and Methods, 2019, 29(8):569-579

Han Z, Li Y, Zhang S, et al. Prenatal transfer of decabromodiphenyl ether (BDE-209) results in disruption of the thyroid system and developmental toxicity in zebrafish offspring[J]. Aquatic Toxicology, 2017, 190:46-52

Hoffman K, Lorenzo A, Butt C M, et al. Exposure to flame retardant chemicals and occurrence and severity of papillary thyroid cancer:A case-control study[J]. Environment International, 2017, 107:235-242

Stapleton H M, Misenheimer J, Hoffman K, et al. Flame retardant associations between children's handwipes and house dust[J]. Chemosphere, 2014, 116:54-60

Fulton C A, Huff Hartz K E, Fell R D, et al. An assessment of pesticide exposures and land use of honey bees in Virginia[J]. Chemosphere, 2019, 222:489-493

Dong W, Wang F, Fang M, et al. Use of biological detection methods to assess dioxin-like compounds in sediments of Bohai Bay, China[J]. Ecotoxicology and Environmental Safety, 2019, 173:339-346

康桂英, 董武, 杨景峰, 等. 对乙酰氨基酚对斑马鱼胚胎甲状腺激素相关基因表达的影响[J]. 中国药理学与毒理学杂志, 2019, 33(4):288-294Kang G Y, Dong W, Yang J F, et al. Effect of acetaminophen on expression of thyroid hormone related genes in zebrafish embryo[J]. Chinese Journal of Pharmacology and Toxicology, 2019, 33(4):288-294(in Chinese)

李嘉伟, 尹晓宇, 周旖妮, 等. 五溴联苯醚(BDE-99)和羟基五溴联苯醚(5-OH-BDE-99)经由THRβ影响斑马鱼胚胎眼部色素的沉着[J]. 生态毒理学报, 2021, DOI:10.7524/AJE.1673-5897.20191114001 Li J W, Yin X Y, Zhou Y N, et al. BDE-99 and 5-OH-BDE-99 affect the pigmentation of the eyes of zebrafish embryos via THRβ[J]. Asian Journal of Ecotoxicology, 2021, DOI:10.7524/AJE.1673-5897.20191114001(in Chinese)

Stapleton H M, Letcher R J, Baker J E. Debromination of polybrominated diphenyl ether congeners BDE 99 and BDE 183 in the intestinal tract of the common carp (Cyprinus carpio)[J]. Environmental Science & Technology, 2004, 38(4):1054-1061

Wang B L, Pang S T, Zhang X L, et al. Levels of polybrominated diphenyl ethers in settled house dust from urban dwellings with resident preschool-aged children in Nanjing, China[J]. Archives of Environmental Contamination and Toxicology, 2015, 68(1):9-19

Li Y, Chen L, Wen Z H, et al. Characterizing distribution, sources, and potential health risk of polybrominated diphenyl ethers (PBDEs) in office environment[J]. Environmental Pollution, 2015, 198:25-31

Zheng X, Sun R, Qiao L, et al. Flame retardants on the surface of phones and personal computers[J]. Science of the Total Environment, 2017, 609:541-545

Lee S, Jang Y C, Kim J G, et al. Static and dynamic flow analysis of PBDEs in plastics from used and end-of-life TVs and computer monitors by life cycle in Korea[J]. Science of the Total Environment, 2015, 506-507:76-85

Carlsson G, Kulkarni P, Larsson P, et al. Distribution of BDE-99 and effects on metamorphosis of BDE-99 and -47 after oral exposure in Xenopus tropicalis[J]. Aquatic Toxicology, 2007, 84(1):71-79

Yoo J H, Takeuchi T, Tagawa M, et al. Effect of thyroid hormones on the stage-specific pigmentation of the Japanese flounder Paralichthys olivaceus[J]. Zoological Science, 2000, 17(8):1101-1106

Dong W, Macaulay L J, Kwok K W, et al. Using whole mount in situ hybridization to examine thyroid hormone deiodinase expression in embryonic and larval zebrafish:A tool for examining OH-BDE toxicity to early life stages[J]. Aquatic Toxicology, 2013, 132-133:190-199

Wang C, Zhu J, Zhang Z, et al. Rno-miR-224-5p contributes to 2,2',4,4'-tetrabromodiphenyl ether-induced low triiodothyronine in rats by targeting deiodinases[J]. Chemosphere, 2020, 246:125774