刘建梅2,
丁洁2,
陈丽红3,,
1. 南京财经大学食品科学与工程学院, 南京 210023;
2. 江苏雅信昆成检测科技有限公司, 南京 210034;
3. 南京中医药大学药学院, 南京 210023
作者简介: 杨倩(1986-),女,博士,研究方向为生态毒理学,E-mail:jsyqhappy@126.com.
通讯作者: 陈丽红,clh_helen@njucm.edu.cn
基金项目: 国家重点研发计划资助项目（2018YFC1801501）；国家重点研发计划资助项目（2018YFC1706500）中图分类号: X171.5
Endocrine Disrupting Effects of Bisphenol F on Early Life Stages of Zebrafish
Yang Qian1,Liu Jianmei2,
Ding Jie2,
Chen Lihong3,,
1. College of Food Science and Engineering, Nanjing University of Finance and Economics, Nanjing 210023, China;
2. Jiangsu Yaxin Tech. Co. Ltd., Nanjing 210034, China;
3. School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, China
Corresponding author: Chen Lihong,clh_helen@njucm.edu.cn
CLC number: X171.5
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摘要:双酚A （bisphenol，BPA）的内分泌干扰性导致许多国家出台了管控措施，双酚F （bisphenol F，BPF）作为其替代物被大量使用，并广泛存在于水体和食品中，导致人群和野生动物长期处于其慢性暴露过程中，可能会威胁人类和生态健康。以斑马鱼胚胎为研究模型，将其暴露于不同浓度的BPF中至受精后144 h （hours post fertilization，hpf），研究BPF对斑马鱼胚胎发育阶段的内分泌干扰作用。结果表明，BPF能够导致斑马鱼的畸形率升高，且具有剂量-效应关系。斑马鱼胚胎暴露于100 μg·L-1和1 000 μg·L-1 BPF后，引起了三碘甲状腺原氨酸（triiodothyronine，T3）水平升高，甲状腺素（thyroxine，T4）和类固醇皮质醇（cortisol，C）水平降低；而10 μg·L-1以上浓度BPF导致17β-雌二醇（17β-estradiol，E2）的水平显著性升高，睾酮（testosterone，T）水平显著性降低。另外，BPF导致下丘脑-垂体-甲状腺（hypothalamic-pituitary-thyroid，HPT）轴、下丘脑-垂体-性腺（hypothalamic-pituitary-gonadal，HPG）轴和下丘脑-垂体-肾上腺（hypothalamic-pituitary-adrenal，HPA）轴上一系列基因表达水平发生改变，这些改变会影响斑马鱼的内分泌功能，进而可能会对生物体的生长发育产生影响。
关键词: 双酚F/
斑马鱼/
内分泌干扰效应/
下丘脑-垂体-甲状腺轴/
下丘脑-垂体-性腺轴/
下丘脑-垂体-肾上腺轴
Abstract:Bisphenol A (BPA) has been banned in certain products due to its endocrine disrupting effects. Because of the restrictions, bisphenol F (BPF) has been developed to substitute BPA in the manufacture of polycarbonate and epoxy resins. The increased application of BPF has resulted in ubiquitous food and environmental pollution, thus humans and wild animals are the most likely to be under chronic exposure. In this study, zebrafish embryos were exposed to BPF from 2 to 144 hours post fertilization (hpf) to investigate the endocrine disrupting effects of BPF on early life stages of zebrafish. The results revealed that BPF increased the mortality in a dose-dependent manner. Exposure to 100 μg·L-1 and 1 000 μg·L-1 BPF increased triiodothyronine (T3) and 17β-estradiol (E2) levels, and decreased thyroxine (T4) and cortisol (C) levels. 10, 100 and 1 000 μg·L-1 BPF increased E2 levels, and decreased testosterone (T) levels. Expression of related genes along the hypothalamic-pituitary-thyroid (HPT), hypothalamic-pituitary-gonadal (HPG) and hypothalamic-pituitary-adrenal (HPA) axes were also evaluated. Changes of gene expression in the HPT, HPG and HPA axes caused by BPF can affect the endocrine function of zebrafish, which may affect the growth and development of zebrafish.
Key words:bisphenol F/
zebrafish/
endocrine disrupting effects/
hypothalamic-pituitary-thyroid axis/
hypothalamic-pituitary-gonadal axis/
hypothalamic-pituitary-adrenal axis.
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