黄沛力1,,
1. 首都医科大学公共卫生学院, 北京 100069;
2. 包头医学院公共卫生学院, 包头 014040
作者简介: 张凌燕(1986-),女,博士研究生,研究方向为分析毒理学,E-mail:zhanglingyan680@139.com.
通讯作者: 黄沛力,huangpl@ccmu.edu.cn
基金项目: 国家自然科学基金资助项目（81573201，81872667）中图分类号: X171.5
Capillary Electrophoresis: Application and Progress for Toxicity Research of Nanomaterials
Zhang Lingyan1,2,Huang Peili1,,
1. School of Public Health, Capital Medical University, Beijing 100069, China;
2. School of Public Health, Baotou Medical College, Baotou 014040, China
Corresponding author: Huang Peili,huangpl@ccmu.edu.cn
CLC number: X171.5
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摘要:纳米材料（nanomaterials，NMs）在各个领域的广泛应用导致其不可避免地通过环境暴露、职业暴露和医源性暴露进入人体。当NMs进入人体后，生理环境中复杂的生物分子都可能与NMs发生相互作用，不仅使NMs获得全新的生物学特性而影响其潜在毒性，还可能改变生物分子的结构和生物学功能而引发疾病。随着对NMs毒性效应研究的不断深入，能够详细描述NMs、生物分子以及NMs和生物分子相互作用形成的复合物的分析技术受到广泛关注。毛细管电泳技术（capillary electrophoresis，CE）以其高灵敏、高分辨、高通量、条件温和以及低消耗的优势在NMs与生物分子相互作用研究领域展现出巨大潜力。本文阐述了2010—2019年间CE技术研究NMs与蛋白质相互作用的动态行为表征、结合平衡分析、蛋白冠的形成和转换监测，以及NMs与其他生物分子相互作用的新进展。
关键词: 纳米材料/
生物分子/
相互作用/
毛细管电泳
Abstract:Nanomaterials (NMs) would enter human body through environmental, occupational and iatrogenic exposures due to their wide application. NMs would interact with the biomolecules in physiological environment after internalization. Consequently, NMs acquire novel biological characters that would affect their potential toxicity, and the structure and biological function of biomolecules might also be altered that would cause disease. With the study on the toxic effects of NMs going on deeply, the analysis techniques that can describe NMs, biomolecules and the complexes formed by their interaction are given more concern. Capillary electrophoresis (CE), with its advantages of high sensitivity, high resolution, high throughput, mild conditions and low consumption, has shown great potential in the research field of interaction between NMs and biomolecules. This review focuses on the application and progress of CE in exploring the interaction between NMs and proteins from 2010 to 2019, including characterizing the dynamic behavior, analyzing the binding balance, monitoring the formation and transformation of protein corona, as well as the interaction of NMs with other biomolecules.
Key words:nanomaterial/
biomolecules/
interaction/
capillary electrophoresis.
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