响应面法优化鹿茸胶原蛋白制备抗氧化肽的水解条件

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兰亚茹¹，黄硕¹，赵飞²，吴宏宇¹，郭永学^1*

1. 沈阳药科大学制药工程学院，辽宁沈阳 1100162. 辽宁省检验检测认证中心，辽宁沈阳 110015

收稿日期:2019-03-25修回日期:2019-05-20出版日期:2020-01-22发布日期:2020-01-14
通讯作者:郭永学

Optimization of enzymatic hydrolysis conditions for antioxidant peptide preparation from velvet antler collagen by response surface methodology

Yaru LAN¹, Shuo HUANG¹, Fei ZHAO², Hongyu WU¹, Yongxue GUO^1*

1. School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China2. Liaoning Inspection, Examination & Certification Centre, Shenyang, Liaoning 110015, China

Received:2019-03-25Revised:2019-05-20Online:2020-01-22Published:2020-01-14
Contact:GUO　Yong-Xue

摘要/Abstract

摘要： 以鹿茸中下段胶原蛋白为酶解底物，用木瓜蛋白酶酶解制备小分子抗氧化肽，以清除1,1-二苯基-2-苦基肼基(DPPH?)自由基的能力为指标，采用响应面法优化酶水解条件。结果表明，最优实验条件为时间56 min，酶添加量1.40wt%，pH=5.60，温度60℃。该条件下所得抗氧化肽对DPPH?自由基的清除率为83.09%。用超滤膜、半制备色谱柱和超高效液相色谱?质谱联用仪分级分离获得分子量0.2?0.6 kDa的具有最高抗氧化活性的多肽，其具有与头段类似的保健功效，更易被人体吸收，且易进一步加工和储存。

引用本文

兰亚茹黄硕赵飞吴宏宇郭永学. 响应面法优化鹿茸胶原蛋白制备抗氧化肽的水解条件[J]. 过程工程学报, 2020, 20(1): 91-98.
Yaru LAN Shuo HUANG Fei ZHAO Hongyu WU Yongxue GUO. Optimization of enzymatic hydrolysis conditions for antioxidant peptide preparation from velvet antler collagen by response surface methodology[J]. Chin. J. Process Eng., 2020, 20(1): 91-98.

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