邢慧芳^1,2， 杨良嵘^1*， 于杰淼¹， 刘会洲¹， 余 浩³， 李万波³， 崔水东³

1. 中国科学院过程工程研究所绿色过程与工程重点实验室，北京 100190 2. 中国科学院大学化学工程学院，北京 100049 3. 盘古基因生物工程(南京)股份有限公司，江苏 南京 210000

收稿日期:2018-06-28修回日期:2018-08-10出版日期:2018-12-22发布日期:2018-12-19
通讯作者:邢慧芳

基金资助:国家自然科学基金;国家自然科学基金重点项目;国家科技支撑计划;中国科学院青年创新促进会资助项目

Effect of magnetic Fe₃O₄@SiO₂ nanoparticles structure on its adsorption to DNA

Huifang XING^1,2, Liangrong YANG^1*, Jiemiao YU¹, Huizhou LIU¹, Hao YU³, Wanbo LI³, Shuidong CUI³

1. Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China 2. School of Chemical Engineering, University of Chinese Academy of Sciences, Beijing 100049, China 3. PangoGene BioScience Ltd., Nanjing, Jiangsu 210000, China

Received:2018-06-28Revised:2018-08-10Online:2018-12-22Published:2018-12-19

摘要/Abstract

摘要： 采用共沉淀法和水热法制备了不同结构的超顺磁性Fe3O4@SiO2纳米颗粒，对其进行表征，研究了其吸附DNA的性能及磁分离性能. 结果表明，20?750 nm范围内粒径较大的颗粒与DNA结合时可提供更多单位平面结合位点，使结合的稳定性和结合几率增加，DNA结合量提高. 不同核?壳结构的Fe3O4@SiO2纳米颗粒的磁分离响应时间不同，内核大小相近时，壳层厚度增加会导致颗粒在磁场中受到的磁力与阻力的比值减小，磁响应时间增加，DNA回收率降低. 粒径约为200 nm的Fe3O4@SiO2纳米颗粒用于纯化全血中DNA最好，提取率为95.2%，磁响应时间为10 s.

引用本文

邢慧芳 杨良嵘 于杰淼 刘会洲 余浩 李万波 崔水东. 磁性Fe₃O₄@SiO₂颗粒结构对其吸附DNA的影响[J]. 过程工程学报, 2018, 18(6): 1119-1125.
Huifang XING Liangrong YANG Jiemiao YU Huizhou LIU Hao YU Wanbo LI Shuidong CUI. Effect of magnetic Fe₃O₄@SiO₂ nanoparticles structure on its adsorption to DNA[J]. Chin. J. Process Eng., 2018, 18(6): 1119-1125.

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