摘要/Abstract

二氧化硫(SO₂)及其衍生物(亚硫酸盐、亚硫酸氢盐等)作为活性硫物种的重要组成部分, 与生命体的生理过程有着密切的关系. 此外, 摄入过量的SO₂会对呼吸系统造成不可修复的损伤, 例如呼吸系统损伤、心脑血管和神经疾病等. 近红外发射荧光探针具有较强的组织穿透能力, 可以有效地检测活体内的生物分子. 因此, 设计了一种基于迈克尔加成(Michael Addition)机理的线粒体靶向近红外SO₂荧光探针XA-SO₂. 溶液光谱实验表明, 该探针XA-SO₂对SO₂有着显著的选择性和灵敏度, 并且具有快速检测SO₂的能力. 值得注意的是, 该荧光探针XA-SO₂具有较好的细胞膜渗透能力, 成功检测到了细胞外/内源性SO₂. 更重要的是, 该探针XA-SO₂能够有效地在细胞的线粒体上富集, 有望实现细胞线粒体内SO₂的动态检测.
关键词: 二氧化硫, 荧光探针, 生物成像, 迈克尔加成, 线粒体
Sulfur dioxide (SO₂) and its derivatives (sulfites, bisulfites, etc.), as important components of active sulfur species, are closely related to the physiological processes of living organisms. In addition, excessive intake of SO₂ may cause irreparable damage to the respiratory system, such as lung cancer, tracheitis, asthma, etc. Near-infrared emission fluorescent probes have strong penetrating ability and can detect biomolecules in vivoeffectively. Therefore, in this paper, a mitochondrial targeted near-infrared SO₂ fluorescent probe based on the Michael addition mechanism was designed. The solution spectroscopic experiments show that XA-SO₂ has significant selectivity and sensitivity to SO₂, and has the ability to rapidly detect SO₂. It is worth noting that XA-SO₂ has good membrane permeability and has successfully detected extracellular/endogenous levels. More importantly, XA-SO₂ could be enriched effectively on the mitochondria of cells, which is promising for realizing the dynamic detection of SO₂ in the mitochondria of living cells.
Key words: sulfur dioxide, fluorescent probe, bioimaging, Michael addition, mitochondria


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