摘要/Abstract

通过两步反应合成了一种2-(3-氰基呋喃-2(5H)-亚甲基)丙二腈衍生物TCF-Pyn, 该化合物在MeCN/磷酸缓冲容溶液(PBS) (V∶V=2∶8, PBS 2×10^–2 mol/L, pH=7.4)混合溶液中能高选择性荧光“OFF-ON”识别Pd²⁺, 发射波长达到近红外区(653 nm). 该探针具有较好的抗干扰能力、较宽的pH适用范围(3~8)和较低的检测限(6.92 μmol/L). 应用研究表明, TCF-Pyn能检测真实水样中的Pd²⁺, 并可对活细胞中的Pd²⁺进行荧光成像.
关键词: 荧光探针, 近红外发射, 识别, Pd²⁺, 细胞成像
2-(3-Cyano-furan-2(5H)-methylene)malononitrile derivative (TCF-Pyn) was synthesized by a two-step reaction. Compound TCF-Pyn could recognize Pd²⁺ with a high selectivity, “OFF-ON” fluorescent response and the near-infrared emission (653 nm) in MeCN/PBS ( V∶V=2∶8, PBS 2×10^–2 mol/L, pH=7.4) solution. The probe possesses a good anti- interference capability, a wide pH range (3~8) and a low detection limit (6.92 μmol/L). The application research showed that TCF-Pyn could detect Pd²⁺ in real water samples and fluorescent image Pd²⁺in living cells.
Key words: fluorescent probe, near-infrared emission, recognition, Pd²⁺, cell imaging


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