摘要/Abstract

以苯磺酰肼和4-二苯胺基苯甲醛为原料, 通过缩合反应设计和合成了一种新型磺酰腙汞离子(Hg²⁺)探针N'-(4-(二苯氨基)亚苄基)苯磺酰腙(S1). 在二甲基亚砜(DMSO)/H₂O (V∶V=7∶3, pH=7)溶液体系中,S1对Hg²⁺展示出快速响应、高选择和高荧光灵敏性. 通过¹H NMR和HRMS探究了可能的机理. 聚丙烯酰胺(PAM)掺杂S1 (PAMS)对Hg²⁺有高的吸附性能, 其去除率达到99.99%, 并且在紫外灯下通过肉眼很容易区分. 通过扫描电子显微镜(SEM)观察了PAMS吸附前后的微观形貌. 此外, S1具有优异的生物相容性, 可以极好地检测HeLa细胞中的Hg²⁺.
关键词: 磺酰腙, 汞离子, 聚丙烯酰胺掺杂, 吸附, HeLa细胞
A novel sulfonylhydrazone Hg²⁺ probe (4-(diphenylamino)benzylidene)benzenesulfonyl hydrazone (S1) was designed and synthesized by condensation reaction between benzenesulfonyl hydrazide and 4-(diphenylamino)benzaldehyde. Probe S1 exhibited a rapid response time, high selectivity and sensitivity fluorescent response to Hg²⁺ in dimethyl sulfoxide (DMSO)/H₂O (V∶V=7∶3, pH=7) solution. The possible mechanism was studied by¹H NMR and HRMS. Polyacrylamide (PAM) doped S1 (PAMS) had high adsorption for Hg²⁺ with removal rate of 99.99% and could easily be distinguished by the naked-eye in UV lamp. And, the micro-morphology of PAMS before and after adsorption was observed by scanning electron microscope (SEM). In addition, probe S1 had excellent biocompatibility and could primely detected Hg²⁺ in HeLa cells.
Key words: sulfonylhydrazone, mercury, polyacrylamide doping, adsorption, HeLa cell


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