摘要/Abstract

利用水溶性磷酸盐柱[5]芳烃（PP5A）作为主体，指示染料亚甲基蓝（MB）作为客体，构筑了MB/PP5A主-客体包合物，研究了PP5A与MB包合时的荧光性质、络合常数、络合比以及包合模式，并将包合物运用于百草枯（PQ）的竞争性荧光传感.MB与PP5A受体络合后，MB的荧光发生淬灭，将PQ加入到MB/PP5A传感系统中，PQ竞争进入PP5A的空腔中，而MB从PP5A的空腔中脱离导致荧光恢复，以此来实现对PQ的竞争性荧光传感.因此，MB/PP5A荧光传感系统可以灵敏地检测PQ，最低检测限为3.6×10^-7 mol·L^-1.该探针具有选择性强、制备简便快捷、较宽pH范围下响应和良好的抗干扰性能等优点，为生物体及环境中PQ的传感检测提供基础.
关键词: 水溶性磷酸盐柱[5]芳烃, 亚甲基蓝, 包合物, 竞争识别, 百草枯
Using water-soluble phosphate salt pillar[5]arene (PP5A) as the host and indicate dye methylene blue (MB) as the guest, the MB/PP5A host-guest inclusion complex was constructed. The fluorescence properties, complexation constants, complexation ratios and binding modes of PP5A and MB were studied. The inclusion complex was applied to competitive fluorescent sensing of paraquat (PQ). When MB is complexed with PP5A receptor, its fluorescence is quenched. When PQ is added to the MB/PP5A sensing system, PQ competes into the cavity of PP5A and MB is detached from the cavity of PP5A, which led to fluorescence recovery. This enables competitive fluorescence sensing of PQ. Therefore, the MB/PP5A fluorescence sensing system can detect PQ sensitively with a minimum detection limit of 3.6×10^-7mol·L^-1. The probe has the advantages of strong selectivity, simple and fast preparation, response in a wide pH range and good anti-interference performance, and can provide the basis for the sensing detection of PQ in organisms and environments.
Key words: water-soluble phosphate salt pillar[5]arene, methylene blue, inclusion complex, competitive recognition, paraquat


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