摘要/Abstract

硫化氢（H₂S）和pH在环境和细胞内的许多过程中起着至关重要的作用.为了更好地研究其功能，需要一种快速、灵敏地检测H₂S和pH的荧光探针.设计合成了以萘二酰亚胺为荧光基团，叠氮为识别位点，基于分子内电荷转移（ICT）机制的反应型荧光探针L.该探针L在较宽的pH范围（4~11）内稳定性好，对H₂S的选择性高，响应速度快（3 min），检测限（1.18 μmol·L^-1）较低，在0~20 μmol·L^-1的范围内，荧光强度与H₂S浓度呈良好的线性关系（R²=0.99823）.此外，当体系中存在30倍的H₂S时，在2 ≤ pH ≤ 6.5的范围内，L对pH变化显示线性关系（R²=0.98764），可用作pH探针.该探针在检测H₂S和30倍H₂S存在条件下研究细胞微环境pH变化的方法，在分析检测和病理分析等方面具有潜在的应用前景.
关键词: 硫化氢, pH, 荧光探针, 理论计算, ICT机制
Hydrogen sulfide (H₂S) and pH play a most important role in vivo and environment. A fluorescent probe for fast and sensitive detection of H₂S and pH are of vital importance for research their functions. A naphthalimide-based fluorescent probe L has been designed and synthesized based on intramolecular charge transfer (ICT) mechanism by utilizing azide as recognition site. The probe L has demonstrated good stability in the pH range of 4~11. It showed satisfactory sensitivity and rapidly response (less than 3 min) to H₂S. An excellent linear relationship (R²=0.99823) displayed in the range of H₂S concentrations from 0 μmol·L^-1 to 20 μmol·L^-1 and the detection limit was 1.18 μmol·L^-1. Furthermore, in the present 30 equiv. H₂S, it was successfully applied to detection of pH with good linear relationship (R²=0.98764) in the pH range of 2~6.5. This methods provide a reference for detection of H₂S and pH value in the presence of 30 equiv. H₂S, which have potential application prospects in analytical detection and pathological analysis.
Key words: hydrogen sulfide, pH, fluorescent probe, theoretical calculations, ICT mechanism


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