摘要/Abstract

过氧亚硝酸根（ONOO^-）是生物体内的一种重要的活性氧，与人体内的各种生理活动息息相关.利用苯并吡喃腈-香豆素体系，合成了一种用于ONOO^-检测的近红外比率型荧光探针（E）-7-（二乙胺基）-3-（2-（苯并吡喃腈）乙烯基）-香豆素（DCCM）.该探针在ONOO^-存在下表现出强烈的响应，颜色从紫色变为浅粉色，最大发射峰蓝移217 nm，荧光颜色从淡紫色变为蓝色，能够直观地对溶液中的ONOO^-进行监测.DCCM可以灵敏地检测ONOO^-，最低检测限为6.0×10^-7 mol·L^-1，该探针被成功用于HeLa细胞中的ONOO^-成像检测.
关键词: 荧光探针, 比率探针, 过氧亚硝酸盐, 细胞成像
Peroxynitrite (ONOO^-) is an important reactive oxygen species in living organisms, and is associated with various biological process in human body. Using dicyanomethylene-4H-pyran coumarin system, a near-infrared ratiometric fluorescent probe (E)-7-(diethylamino)-3-(2-(dicyanomethylene-4H-pyran)vinyl)-coumarin (DCCM) was developed for ONOO^- detec-tion. This probe exhibited a strong response in the presence of ONOO^-, with a significant color change from purple to light pink, along with 217 nm fluorescence blue shift. The emission color changed from heliotrope to blue, which enables the monitoring of ONOO^- by naked eyes. DCCM was used to sensitively measure ONOO^- with low detection limit of 6.0×10^-7 mol·L^-1. The use of DCCM for imaging ONOO^- in HeLa cells was also demonstrated.
Key words: fluorescence probe, ratiometric probe, peroxynitrite, cell imaging


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