Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 王利军博士 (妇产科学系) |
| 王华婷教授 (李嘉诚健康科学研究所) |
| 孙昊教授 (化学病理学系) |
| 周良博士 (妇产科学系) |
| 王利军博士 (妇产科学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1038/mt.2012.35 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/44WOS source URL
其它资讯
摘要microRNAs (miRNAs) are noncoding RNAs that regulate gene expression in post-transcriptional fashion, and emerging studies support their importance in a multitude of physiological and pathological processes. Here, we describe the regulation and function of miR-29 in Duchenne muscular dystrophy (DMD) and its potential use as therapeutic target. Our results demonstrate that miR-29 expression is downregulated in dystrophic muscles of mdx mice, a model of DMD. Restoration of its expression by intramuscular and intravenous injection improved dystrophy pathology by both promoting regeneration and inhibiting fibrogenesis. Mechanistic studies revealed that loss of miR-29 in muscle precursor cells (myoblasts) promotes their transdifferentiation into myofibroblasts through targeting extracellular molecules including collagens and microfibrillar-associated protein 5 (Mfap5). We further demonstrated that miR-29 is under negative regulation by transforming growth factor-beta (TGF-beta) signaling. Together, these results not only identify TGF-beta-miR-29 as a novel regulatory axis during myoblasts conversion into myofibroblasts which constitutes a novel contributing route to muscle fibrogenesis of DMD but also implicate miR-29 replacement therapy as a promising treatment approach for DMD.
着者Wang LJ, Zhou L, Jiang PY, Lu LN, Chen XN, Lan HY, Guttridge DC, Sun H, Wang HT
期刊名称Molecular Therapy
详细描述To ORKTS: duplicated record
出版年份2012
月份6
日期1
卷号20
期次6
出版社Nature Publishing Group: Open Access Hybrid Model Option B
页次1222 - 1233
国际标準期刊号1525-0016
电子国际标準期刊号1525-0024
语言英式英语
Web of Science 学科类别Biotechnology & Applied Microbiology; BIOTECHNOLOGY & APPLIED MICROBIOLOGY; Genetics & Heredity; GENETICS & HEREDITY; Medicine, Research & Experimental; MEDICINE, RESEARCH & EXPERIMENTAL; Research & Experimental Medicine
