Publication in refereed journal
香港中文大学研究人员 ( 现职)
| 孙昊教授 (化学病理学系) |
全文
| 数位物件识别号 (DOI) http://dx.doi.org/10.1093/nar/gkq775 |
引用次数
Web of Sciencehttp://aims.cuhk.edu.hk/converis/portal/Publication/34WOS source URL
Scopushttp://aims.cuhk.edu.hk/converis/portal/Publication/34Scopus source URL
其它资讯
摘要Alternative promoters that are differentially used in various cellular contexts and tissue types add to the transcriptional complexity in mammalian genome. Identification of alternative promoters and the annotation of their activity in different tissues is one of the major challenges in understanding the transcriptional regulation of the mammalian genes and their isoforms. To determine the use of alternative promoters in different tissues, we performed ChIP-seq experiments using antibody against RNA Pol-II, in five adult mouse tissues (brain, liver, lung, spleen and kidney). Our analysis identified 38639 Pol-II promoters, including 12270 novel promoters, for both protein coding and non-coding mouse genes. Of these, 6384 promoters are tissue specific which are CpG poor and we find that only http://aims.cuhk.edu.hk/converis/portal/Publication/34 of the novel promoters are located in CpG-rich regions, suggesting that novel promoters are mostly tissue specific. By identifying the Pol-II bound promoter(s) of each annotated gene in a given tissue, we found that 37 of the protein coding genes use alternative promoters in the five mouse tissues. The promoter annotations and ChIP-seq data presented here will aid ongoing efforts of characterizing gene regulatory regions in mammalian genomes. ? 2010 The Author(s).
着者Sun H., Wu J., Wickramasinghe P., Pal S., Gupta R., Bhattacharyya A., Agosto-Perez F.J., Showe L.C., Huang T.H.-M., Davuluri R.V.
期刊名称Nucleic Acids Research
出版年份2011
月份1
日期17
卷号39
期次1
出版社Oxford University Press
出版地United Kingdom
页次190 - 201
国际标準期刊号0305-1048
电子国际标準期刊号1362-4962
语言英式英语
