| |
| 文章导读 |
|
摘要提高表面等离子共振(surface plasmon resonance, SPR)检测系统的分辨率有利于扩大其应用。该文围绕角度检测方法,从理论上阐明了检测系统的分辨率不仅随着灵敏度的增强而改善,而且随着半峰宽的变窄而提高。同时,深入分析了使用金、银以及金银膜的分辨率,明确金银膜的分辨率比金膜高,提出采用金银膜的芯片来提高系统的分辨率。以不同质量分数的NaCl溶液为介质,分别测量使用金膜和金银膜时的分辨率。实验结果表明,金膜和金银膜各自对应的分辨率为5.2×10-6 RIU (refractive index unit)和 3.9×10-6 RIU, 后者较前者提高1.4倍,与理论分析结果1.6倍接近。
|
| 关键词 ：表面等离子共振(SPR),系统分辨率,灵敏度,金银膜 |
Abstract：Improved system resolutions for surface plasmon resonance (SPR) will enable applications in more fields. This paper shows theoretically that the resolution of SPR with angular modulation is not only improved with increased sensitivity, but also with a narrower half-peak width. The resolutions with Au, Ag and Au-Ag chips show that the resolution of the Au-Ag chip is higher than that of the Au chip. Thus, the system resolution can be improved by using the Au-Ag chip. Measurements of the resolution of Au and Au-Ag chips using NaCl solutions with different mass fractions show that the Au and Au-Ag chip resolutions are 5.2×10-6 and 3.9×10-6 RIU (refractive index unit). Thus, the Au-Ag chip resolution is 1.4 times higher than the Au chip, similar to the theoretical result being 1.6 times higher.
|
| Key words：surface plasmon resonance (SPR)system resolutionsensitivityAu-Ag chip |
| 收稿日期: 2013-03-07 出版日期: 2015-04-16 |
|
| 基金资助:国家自然科学基金资助项目 (30970757) |
| [1] | Myszka D G, Rebecca L R. Implementing surface plasmon resonance biosensors in drug discovery[J]. Pharmaceutical Science & Technology Today, 2000, 3(9): 310-316. |
| [2] | Workman P, Aboagye E, Balkwill F, et al.Guidelines for the welfare and use of animals in cancer research[J]. British Journal of Cancer, 2010, 102: 1555-1577. |
| [3] | Mulchan N M, Rodriguez M, Kevin O, et al.Application of a high-resolution SPR technique for monitoring real-time metal/dielectric interactions[J]. Sensors and Actuators B, 2003, 88: 132-137. |
| [4] | 定翔, 余兴龙, 耿俊清. 表面等离子体共振生物传感技术及仪器化的过去、 现在与未来[J]. 现代科学仪器, 2012, 3: 124-134. DING Xiang, YU Xinglong, GENG Junqing. Development history of surface plasmon resonance biosensor technology and instrumentation[J]. Modern Scientific Instruments, 2012, 3: 124-134. (in Chinese) |
| [5] | Zynio S A, Samoylov A V, Surotseva E R, et al.Bimetallic layers increase sensitivity of affinity sensor based on surface plasmon resonance[J]. Sensors, 2002, 2: 62-70. |
| [6] | Biow H O, YUAN Xiaocong, Tjin S C, et al.Optimised film thickness for maximum evanescent fild enhancement of a bimetallic film surface plasmon resonance biosensor[J]. Sensors and Actuators B, 2006, 114: 1028-1034. |
| [7] | Johansen K, Stalbert R, Lundstrom I, et al.Surface plasmon resonance: Instrumental resolution using photo diode array[J]. Measurement Science and Technology, 2000, 11: 1630-1638. |
| [8] | Tao N J, Boussad S, Huang W L, et al.High resolution surface plasmon resonance spectroscopy[J]. Review of Scientific Instrument, 1999, 70(12): 4656-4600. |
| [9] | Ekgasit S, Thammacharoen C, YU Fang, et al.Evanescent field in surface plasmon resonance and surface plasmon field-enhanced fluorescence spectroscopies[J]. Analytical Chemistry, 2004, 76(8): 2210-2219. |
| [10] | Piliarik M, Homola J. Surface plasmon resonance (SPR) sensors: Approaching their limits?[J]. Optics Express, 2009, 17: 16505-16517. |
| [11] | Homola J, Koudela I, Yee S S. Surface plasmon resonance sensors based on diffraction gratings and prism couplers: Sensitivity comparison[J]. Sensors and Actuators B, 1999, 54: 16-24. |
| [12] | Bruijn H E, Kooyman R P H, Greve J. Choice of metal and wavelength for surface-plasmon resonance sensors: Some considerations[J]. Applied Optics, 1992, 31(4): 440-442. |
| [13] | Ekgasit S, YU Fang, Knoll W. Fluorescence intensity in surface-plasmon field-enhanced fluorescence spectroscopy[J]. Sensors and Actuators B, 2005, 104: 294-301. |
| [14] | 耿俊清, 余兴龙, 章恩耀, 等. 基于相位解算的SPR传感方法及其应用[J]. 仪器仪表学报, 2012, 33(10): 15-21. GENG Junqing, YU Xinglong, ZHANG Enyao, et al.SPR biosensing method based on phase shift algorithm and its application[J]. Chinese Journal of Scientific Instrument, 2012, 33(10): 15-21. (in Chinese) |
| [1] | 王振波, 张君, 罗孙一鸣. 喷水法成型纤维网增强水泥基板材抗弯性能[J]. 清华大学学报（自然科学版）, 2014, 54(5): 551-555. | | [2] | 蔡志鹏, 吴健栋, 汤之南, 张伯奇, 潘际銮, 刘霞. 汽轮机焊接转子接头低周疲劳过程损伤变量的复合分析法[J]. 清华大学学报（自然科学版）, 2014, 54(2): 178-184. | | [3] | 刘佳君, 孙振国, 张文增, 陈强. 两端吸附式爬壁机器人机械臂运动误差修正算法[J]. 清华大学学报（自然科学版）, 2014, 54(2): 185-190. | | [4] | 赵海燕, 徐兴全, 于兴哲, 朱小武. 旋挖钻机钻杆键条焊接接头的残余应力[J]. 清华大学学报（自然科学版）, 2014, 54(2): 191-196. | | [5] | 彭卓, 邓焱, 马骋, 熊剑平, 尹永利. 基于FPGA的高精度正弦信号发生器设计与实现[J]. 清华大学学报（自然科学版）, 2014, 54(2): 197-201. | | [6] | 杨杰, 翁文国. 基于改进无偏灰色模型的燃气供气量的预测[J]. 清华大学学报（自然科学版）, 2014, 54(2): 145-148. | | [7] | 黄超, 黄全义, 申世飞, 疏学明. 突发事件案例表示方法[J]. 清华大学学报（自然科学版）, 2014, 54(2): 149-152. | | [8] | 蔡东阳, 卓子寒, 王婕, 武建安, 唐劲天. 基于模拟退火算法的磁感应治疗热籽分布[J]. 清华大学学报（自然科学版）, 2014, 54(2): 153-158. | | [9] | 陈利高, 刘晓波, 龚建, 王侃, 董传江, 荣茹, 鲁艺. 中子多重性探测器搭建及参数标定[J]. 清华大学学报（自然科学版）, 2014, 54(2): 159-163. | | [10] | 苏健, 曾志, 程建平, 李君利. Monte Carlo方法的最优源项偏倚抽样密度函数[J]. 清华大学学报（自然科学版）, 2014, 54(2): 164-171. | | [11] | 刘伟强, 蒲婷, 顾洪生, 廖振华, 姜锦鹏. 中国人颈椎间盘尺寸分析[J]. 清华大学学报（自然科学版）, 2014, 54(2): 172-177. | | [12] | 韩俊, 温风波, 赵广播. 小展弦比涡轮叶片的弯曲优化设计[J]. 清华大学学报（自然科学版）, 2014, 54(1): 102-108. | | [13] | 赵香花, 宋强, 刘文华. 35 kV链式STATCOM及其应用[J]. 清华大学学报（自然科学版）, 2014, 54(1): 109-113. | | [14] | 戴秋娟. 外资企业管理层的国企经验有效性分析——以在华日资企业为例(Ⅰ)[J]. 清华大学学报（自然科学版）, 2014, 54(1): 114-120. | | [15] | 戴秋娟. 国营企业经验对外企管理层职业能力形成的影响分析——以在华日资企业为例(Ⅱ)[J]. 清华大学学报（自然科学版）, 2014, 54(1): 121-132. |
|
)
