Influences of different regulating strategies on the fast adaptation to ammonia nitrogen concentration improvement in CANON process
ZHANG Zeyu1,, WANG Jianfang1,2,3,,, QI Zekun1, QIAN Feiyue1,2, SHEN Yaoliang1,2, YOU Lan4, HUANG Jihui4 1.School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China 2.National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, China 3.Tianping College, Suzhou University of Science and Technology, Suzhou 215009, China 4.Suzhou Drainage Company Limited, Suzhou 215001, China
Abstract:Fluctuations of ammonia nitrogen concentrations in the influent are related to system instability caused by the synergies among functional microbes in granular sludge of the completely autotrophic nitrogen removal over nitrite (CANON) process. In this study, the autotrophic denitrification granular sludge stored at room temperature for 2 months was used as the inoculum. Three control strategies were adopted: rapidly increasing influent ammonia nitrogen concentration (R1) or stepwise increasing influent ammonia nitrogen concentration (R2) at stable HRT, and gradually increasing influent ammonia nitrogen load while adjusting HRT (R3). At the increase of influent ammonia nitrogen concentration by 125 mg·L?1, the effects of three control strategies on the adaption to ammonia nitrogen concentrations of 275 mg·L?1 and 400 mg·L?1 in the CANON process were studied, as well as the relations between various strategies and sludge properties and the effects of free ammonia (FA) and dissolved oxygen (DO). The results show that the strategy of R3 with a stable variation in sludge loading showed the fastest adaptation to the increase of influent ammonia nitrogen concentration during the period of sludge performance improvement, and the total nitrogen removal loading could reach 3.5 kg·(m3·d)?1 within 44 days. In the mature stage of sludge performance, the strategy R1 with rapidly enhancing the influence loading could shorten the adaptation time to 25 days and the total nitrogen removal loading reached 5.3 kg·(m3·d)?1 at the ammonia nitrogen loading of 6.4 kg·(m3·d)?1, and total nitrogen removal rate reached 80%. FA concentration affected the activity of functional microbial. During the sludge performance improvement period, FA concentration of the strategy R1 was as high as 16.6~26.7 mg·L?1, which inhibited the activity of aerobic ammonia oxidizing bacteria (AOB) and anaerobic ammonium oxidation bacteria (AMX) and prolonged system adaptation to some extent. When the sludge was adapted to high ammonia nitrogen loading, specific ammonia oxidation rate (SAOR) and specific nitrogen removal rate (SNRR) gradually increased, and the sludge concentration and particle size gradually increased. The f-value (Δ$ {\rm{NO}}_3^{-}$-N/ΔTN) was used as an important basis for the DO regulation, and a good positive correlation between DO and the ammonia nitrogen removal loading occurred. Key words:CANON granular sludge/ sludge activity/ ammonia nitrogen concentration/ hydraulic retention time/ free ammonia.
图1实验装置示意图 Figure1.Schematic diagram of experimental device
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1.School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China 2.National and Local Joint Engineering Laboratory of Municipal Sewage Resource Utilization Technology, Suzhou 215009, China 3.Tianping College, Suzhou University of Science and Technology, Suzhou 215009, China 4.Suzhou Drainage Company Limited, Suzhou 215001, China Received Date: 2018-12-22 Accepted Date: 2019-04-08 Available Online: 2019-08-06 Keywords:CANON granular sludge/ sludge activity/ ammonia nitrogen concentration/ hydraulic retention time/ free ammonia Abstract:Fluctuations of ammonia nitrogen concentrations in the influent are related to system instability caused by the synergies among functional microbes in granular sludge of the completely autotrophic nitrogen removal over nitrite (CANON) process. In this study, the autotrophic denitrification granular sludge stored at room temperature for 2 months was used as the inoculum. Three control strategies were adopted: rapidly increasing influent ammonia nitrogen concentration (R1) or stepwise increasing influent ammonia nitrogen concentration (R2) at stable HRT, and gradually increasing influent ammonia nitrogen load while adjusting HRT (R3). At the increase of influent ammonia nitrogen concentration by 125 mg·L?1, the effects of three control strategies on the adaption to ammonia nitrogen concentrations of 275 mg·L?1 and 400 mg·L?1 in the CANON process were studied, as well as the relations between various strategies and sludge properties and the effects of free ammonia (FA) and dissolved oxygen (DO). The results show that the strategy of R3 with a stable variation in sludge loading showed the fastest adaptation to the increase of influent ammonia nitrogen concentration during the period of sludge performance improvement, and the total nitrogen removal loading could reach 3.5 kg·(m3·d)?1 within 44 days. In the mature stage of sludge performance, the strategy R1 with rapidly enhancing the influence loading could shorten the adaptation time to 25 days and the total nitrogen removal loading reached 5.3 kg·(m3·d)?1 at the ammonia nitrogen loading of 6.4 kg·(m3·d)?1, and total nitrogen removal rate reached 80%. FA concentration affected the activity of functional microbial. During the sludge performance improvement period, FA concentration of the strategy R1 was as high as 16.6~26.7 mg·L?1, which inhibited the activity of aerobic ammonia oxidizing bacteria (AOB) and anaerobic ammonium oxidation bacteria (AMX) and prolonged system adaptation to some extent. When the sludge was adapted to high ammonia nitrogen loading, specific ammonia oxidation rate (SAOR) and specific nitrogen removal rate (SNRR) gradually increased, and the sludge concentration and particle size gradually increased. The f-value (Δ$ {\rm{NO}}_3^{-}$-N/ΔTN) was used as an important basis for the DO regulation, and a good positive correlation between DO and the ammonia nitrogen removal loading occurred.
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