王华琴1,2,
王敦球1,2
1.桂林理工大学，广西环境污染控制理论与技术重点实验室，桂林 541004
2.桂林理工大学，广西岩溶地区水污染控制与用水安全保障协同创新中心，桂林 541004
基金项目: 国家自然科学基金资助项目51668013，51638006
广西科技计划项目资助桂科AD18126018
桂林市科学研究与技术开发计划项目2016012303
广西矿冶与环境科学实验中心KH2012ZD004
广西高等学校高水平创新团队及卓越****计划项目002401013001国家自然科学基金资助项目(51668013，51638006)
“八桂****”建设工程专项经费
广西科技计划项目资助(桂科AD18126018)
桂林市科学研究与技术开发计划项目(2016012303)
广西矿冶与环境科学实验中心(KH2012ZD004)
广西高等学校高水平创新团队及卓越****计划项目(002401013001)
Migration and transformation of inorganic carbon in the Anammox system
WEI Yuan1,2,,WANG Huaqin1,2,
WANG Dunqiu1,2
1.Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China
2.Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China
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摘要:基于厌氧氨氧化(Anammox)反应，采用13C同位素示踪法分析无机碳(IC)在工艺中的迁移转化路径，考查厌氧氨氧化工艺的固碳潜力及厌氧氨氧化菌相关的固碳机理；同时，结合微生物分子学等方法，通过比较反应前后NH4+-N、NO2--N、TN及IC的变化，分析推导出工艺的固碳机理。结果表明，在进水IC为10.70 mg左右时，系统平均固碳率在12.05%以上；经13C标记处理后的Anammox污泥中13C丰度值由1.07%增加至1.17%以上；Anammox污泥中cbbLR1基因拷贝数经氮素和IC影响后分别为5.79×108 copies·g-1和5.56×108 copies·g-1，较处理前均有所增加，但变化不明显。进水中投加的IC参与了微生物体内的碳代谢；厌氧氨氧化菌存在遵循卡尔文循环固碳途径的功能基因。cbbLR1基因丰度与氮素浓度之间呈显著相关，与IC浓度之间的相关性不明显，说明该基因丰度对氮素的响应度比IC大。
关键词: 厌氧氨氧化/
碳迁移/
13C同位素丰度/
基因丰度检测
Abstract:In this study, the migration and transformation path of inorganic carbon (IC) in Anammox process was identified using the 13C isotope tracer method, and the carbon sequestration potential of Anammox process and the carbon sequestration mechanism of Anammox bacteria were discussed. At the same time, in combination with the microbial molecular methods, the carbon sequestration mechanism of the process was disclosed through comparison of the changes of NH4+-N, NO2--N, TN and IC before and after the reaction. The experimental results showed that the average carbon sequestration rate of the system was above 12.05% when the influent IC was about 10.70 mg. The 13C abundance value of the anaerobic ammonium oxide sludge after 13C labeling treatment increased from 1.07% to over 1.17%. Under the influence of nitrogen and IC, the copy numbers of cbbLR1 gene in Anammox sludge were 5.79×108 copies·g-1 and 5.56×108 copies·g-1 dry sludge, respectively, both of them increased than before treatment, while this change was insignificant. IC added to the influent water participates in the carbon metabolism in the microorganism. Anammox bacteria have some functional genes that follow the Calvin cycle carbon sequestration pathway. The abundance of cbbLR1 gene showed a significant correlation with nitrogen concentration, while an insignificant correlation with IC concentration, indicating that the gene abundance was more responsive to nitrogen than to IC.
Key words:Anammox/
carbon migration/
13C isotopic abundance/
gene abundance detection.
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| [2] | STROUS M, KUENEN J G, JETTEN M S M. Key physiology of anaerobic ammonium oxidation[J]. Applied and Environmental Microbiology, 1999, 65(7): 3248-3250. |
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| [5] | KARTAL B, RATTRAY J, NIFTRIK L A VAN, et al. Candidatus Anammox oglobus propionicus a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria[J]. Systematic and Applied Microbiology, 2007, 30(1): 39-49. |
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| [7] | 朱彤, 梁启煜, 谢元华, 等. 厌氧氨氧化过程中无机碳对脱氮效能的影响[J]. 2018, 39(2): 278-287. |
| [8] | 鲍林林, 茹祥莉, 郭守德, 等. 无机碳对厌氧氨氧化反应的影响[J]. 河南师范大学学报(自然科学版), 2013, 41(4): 90-93. |
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| [10] | HAO X D, LIU R B, HUANG X. Evaluation of the potential for operating carbon neutral WWTPs in China[J]. Water Research, 2015, 87: 424-431. |
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无机碳在厌氧氨氧化系统中的迁移转化
韦愿1,2,,王华琴1,2,
王敦球1,2
1.桂林理工大学，广西环境污染控制理论与技术重点实验室，桂林 541004
2.桂林理工大学，广西岩溶地区水污染控制与用水安全保障协同创新中心，桂林 541004
基金项目: 国家自然科学基金资助项目51668013，51638006 广西科技计划项目资助桂科AD18126018 桂林市科学研究与技术开发计划项目2016012303 广西矿冶与环境科学实验中心KH2012ZD004 广西高等学校高水平创新团队及卓越****计划项目002401013001国家自然科学基金资助项目(51668013，51638006) “八桂****”建设工程专项经费 广西科技计划项目资助(桂科AD18126018) 桂林市科学研究与技术开发计划项目(2016012303) 广西矿冶与环境科学实验中心(KH2012ZD004) 广西高等学校高水平创新团队及卓越****计划项目(002401013001)
关键词: 厌氧氨氧化/
碳迁移/
13C同位素丰度/
基因丰度检测
摘要:基于厌氧氨氧化(Anammox)反应，采用13C同位素示踪法分析无机碳(IC)在工艺中的迁移转化路径，考查厌氧氨氧化工艺的固碳潜力及厌氧氨氧化菌相关的固碳机理；同时，结合微生物分子学等方法，通过比较反应前后NH4+-N、NO2--N、TN及IC的变化，分析推导出工艺的固碳机理。结果表明，在进水IC为10.70 mg左右时，系统平均固碳率在12.05%以上；经13C标记处理后的Anammox污泥中13C丰度值由1.07%增加至1.17%以上；Anammox污泥中cbbLR1基因拷贝数经氮素和IC影响后分别为5.79×108 copies·g-1和5.56×108 copies·g-1，较处理前均有所增加，但变化不明显。进水中投加的IC参与了微生物体内的碳代谢；厌氧氨氧化菌存在遵循卡尔文循环固碳途径的功能基因。cbbLR1基因丰度与氮素浓度之间呈显著相关，与IC浓度之间的相关性不明显，说明该基因丰度对氮素的响应度比IC大。
English Abstract
Migration and transformation of inorganic carbon in the Anammox system
WEI Yuan1,2,,WANG Huaqin1,2,
WANG Dunqiu1,2
1.Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin 541004, China
2.Guangxi Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area, Guilin University of Technology, Guilin 541004, China
Keywords: Anammox/
carbon migration/
13C isotopic abundance/
gene abundance detection
Abstract:In this study, the migration and transformation path of inorganic carbon (IC) in Anammox process was identified using the 13C isotope tracer method, and the carbon sequestration potential of Anammox process and the carbon sequestration mechanism of Anammox bacteria were discussed. At the same time, in combination with the microbial molecular methods, the carbon sequestration mechanism of the process was disclosed through comparison of the changes of NH4+-N, NO2--N, TN and IC before and after the reaction. The experimental results showed that the average carbon sequestration rate of the system was above 12.05% when the influent IC was about 10.70 mg. The 13C abundance value of the anaerobic ammonium oxide sludge after 13C labeling treatment increased from 1.07% to over 1.17%. Under the influence of nitrogen and IC, the copy numbers of cbbLR1 gene in Anammox sludge were 5.79×108 copies·g-1 and 5.56×108 copies·g-1 dry sludge, respectively, both of them increased than before treatment, while this change was insignificant. IC added to the influent water participates in the carbon metabolism in the microorganism. Anammox bacteria have some functional genes that follow the Calvin cycle carbon sequestration pathway. The abundance of cbbLR1 gene showed a significant correlation with nitrogen concentration, while an insignificant correlation with IC concentration, indicating that the gene abundance was more responsive to nitrogen than to IC.
