侯志伟1,
高锦芳1,
谭洪新1,2,3
1.上海海洋大学,上海水产养殖工程技术中心, 上海 201306
2.上海海洋大学,农业部淡水水产种质资源重点实验室, 上海 201306
3.上海海洋大学,水产科学国际级实验教学示范中心, 上海201306
基金项目: 上海市科学技术委员会资助项目(14320501900)
Nitrate removal efficiency and microbial community analysis of polycaprolactone-packed bioreactors with PCL as carbon source treating aquaculture water under different hydraulic retention time
LUO Guozhi1,2,3,,HOU Zhiwei1,
GAO Jinfang1,
TAN Hongxin1,2,3
1.Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306,China
2.Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306,China
3.National Demonstration Center for Experimental Fisheries Science Education, Ministry of Education, Shanghai Ocean University, Shanghai 201306,China
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摘要:以可生物降解聚合为碳源的固相反硝化可以避免水产养殖用水硝酸盐处理过程中碳源反复添加、碳源不足或过量等问题。水力停留时间(hydraulic retention time, HRT)是生物反应器运行管理的主要参数之一, 用固定膜反应器固相反硝化的方法研究了HRT对以聚己内酯(polycaprolactone,PCL)为碳源的反应器去除循环水养殖系统硝酸氮(浓度为170～197 mg·L-1)的效率的影响。 研究结果表明不同水力停留时间对硝酸盐去除效率差异显著。在HRT 为6 h和8 h时,硝酸盐速率分别为(0.55±0.32) g·(L·d)-1和(1.05±0.33) g·(L·d)-1,且出水亚硝氮浓度和氨氮浓度均明显低于进水浓度；在HRT为4 h和2 h时,进出水硝酸盐浓度差异不明显。电子扫描显微镜观察显示PCL表面生物膜主要为杆状菌,应用傅里叶红外扫描观察发现使用前后PCL的化学结构没有发生明显改变。应用高通量方法测定的微生物群落结构表明,62%的细菌为Proteobacteria(62%),在鉴定出的细菌中,食酸菌属(Acidovorax), 固氮螺菌属(Azospira),丛毛单胞菌属(Comamonas), 代尔夫特菌属(Diaphorobacter), 懒小杆属(Ignavibacterium), 弗拉特氏菌属(Frateuria)可以同时降解PCL和进行反硝化。
关键词: 聚己内酯/
固相反硝化/
水力停留时间/
循环水养殖/
硝酸盐
Abstract:A denitrification fixed film biofilter using polycaprolactone (PCL) as carbon source and biofilm carrier was developed to investigate the efficiency of removing nitrate-nitrogen (concentration of 170 to 197 mg·L-1) in water from a recirculating aquaculture system. The hydraulic retention time (HRT) increased from 2 h to 8 h. The removal rates of (1.05±0.33) g·(L·d)-1 was reached at HRT of 8 h and (0.55±0.32) g·(L·d)-1 was reached at HRT of 6 h unused dissolved organic carbon (DOC) from PCL degradation was less than 10 mg·L-1 for all HRTs and increased with HRT. Observation of the PCL surface indicated that chain scission by hydrolytic degradation and biological utilization in the reactors did not change the chemical structure of the PCL significantly. The result of microbial community analysis showed that Proteobacteria was the predominant phylum in the inoculum with the relative abundances of 62%. Acidovorax, Azospira, Comamonas, Diaphorobacter, Ignavibacterium, and Frateuria were capable of both degrading PCL and denitrifying NO-3-N.
Key words:polycaprolactone/
solid-denitrification/
hydraulic retention time/
recirculating aquaculture/
nitrate.
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不同水力停留时间条件下PCL为碳源去除水产养殖水体硝酸盐的效率及微生物群落分析
罗国芝1,2,3,,侯志伟1,
高锦芳1,
谭洪新1,2,3
1.上海海洋大学,上海水产养殖工程技术中心, 上海 201306
2.上海海洋大学,农业部淡水水产种质资源重点实验室, 上海 201306
3.上海海洋大学,水产科学国际级实验教学示范中心, 上海201306
基金项目: 上海市科学技术委员会资助项目(14320501900)
关键词: 聚己内酯/
固相反硝化/
水力停留时间/
循环水养殖/
硝酸盐
摘要:以可生物降解聚合为碳源的固相反硝化可以避免水产养殖用水硝酸盐处理过程中碳源反复添加、碳源不足或过量等问题。水力停留时间(hydraulic retention time, HRT)是生物反应器运行管理的主要参数之一, 用固定膜反应器固相反硝化的方法研究了HRT对以聚己内酯(polycaprolactone,PCL)为碳源的反应器去除循环水养殖系统硝酸氮(浓度为170～197 mg·L-1)的效率的影响。 研究结果表明不同水力停留时间对硝酸盐去除效率差异显著。在HRT 为6 h和8 h时,硝酸盐速率分别为(0.55±0.32) g·(L·d)-1和(1.05±0.33) g·(L·d)-1,且出水亚硝氮浓度和氨氮浓度均明显低于进水浓度；在HRT为4 h和2 h时,进出水硝酸盐浓度差异不明显。电子扫描显微镜观察显示PCL表面生物膜主要为杆状菌,应用傅里叶红外扫描观察发现使用前后PCL的化学结构没有发生明显改变。应用高通量方法测定的微生物群落结构表明,62%的细菌为Proteobacteria(62%),在鉴定出的细菌中,食酸菌属(Acidovorax), 固氮螺菌属(Azospira),丛毛单胞菌属(Comamonas), 代尔夫特菌属(Diaphorobacter), 懒小杆属(Ignavibacterium), 弗拉特氏菌属(Frateuria)可以同时降解PCL和进行反硝化。
English Abstract
Nitrate removal efficiency and microbial community analysis of polycaprolactone-packed bioreactors with PCL as carbon source treating aquaculture water under different hydraulic retention time
LUO Guozhi1,2,3,,HOU Zhiwei1,
GAO Jinfang1,
TAN Hongxin1,2,3
1.Shanghai Engineering Research Center of Aquaculture, Shanghai Ocean University, Shanghai 201306,China
2.Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306,China
3.National Demonstration Center for Experimental Fisheries Science Education, Ministry of Education, Shanghai Ocean University, Shanghai 201306,China
Keywords: polycaprolactone/
solid-denitrification/
hydraulic retention time/
recirculating aquaculture/
nitrate
Abstract:A denitrification fixed film biofilter using polycaprolactone (PCL) as carbon source and biofilm carrier was developed to investigate the efficiency of removing nitrate-nitrogen (concentration of 170 to 197 mg·L-1) in water from a recirculating aquaculture system. The hydraulic retention time (HRT) increased from 2 h to 8 h. The removal rates of (1.05±0.33) g·(L·d)-1 was reached at HRT of 8 h and (0.55±0.32) g·(L·d)-1 was reached at HRT of 6 h unused dissolved organic carbon (DOC) from PCL degradation was less than 10 mg·L-1 for all HRTs and increased with HRT. Observation of the PCL surface indicated that chain scission by hydrolytic degradation and biological utilization in the reactors did not change the chemical structure of the PCL significantly. The result of microbial community analysis showed that Proteobacteria was the predominant phylum in the inoculum with the relative abundances of 62%. Acidovorax, Azospira, Comamonas, Diaphorobacter, Ignavibacterium, and Frateuria were capable of both degrading PCL and denitrifying NO-3-N.
