刘兴国2,
曾宪磊2,
陆诗敏2
1.上海海洋大学水产与生命学院,上海 201306
2.中国水产科学研究院渔业机械仪器研究所,上海 200092
基金项目: 现代农业产业技术体系建设专项(CARS-46)
国家自然科学基金资助项目(31372570)
948节水高效全循环池塘养殖关键技术合作研究(2016-X32)
Effects of organic carbon for denitrification and anaerobic ammonium oxidation in sediments of aquaculture pond
GAO Meiyun1,2,,LIU Xingguo2,
ZENG Xianlei2,
LU Shimin2
1.College of Fisheries and Life Science, Shanghai Ocean University,Shanghai 201306, China
2.Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200092, China
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摘要:养殖沉积物中反硝化作用对于缓解氮污染有重要的作用,沉积物中的反硝化和厌氧氨氧化菌可将化合态氮转变为氮气,从而有效降低污染,有机碳在该过程中有着重要的作用。为了解有机碳对养殖池塘沉积物中反硝化、厌氧氨氧化的影响,采取理化分析和分子生物学分析等方法,以养殖池塘沉积物为基质、人工配水为营养液,添加不同浓度的淀粉,分析120 h内底物亚硝氮(NO-2-N)、硝氮(NO-3-N)、氨氮(NH+4-N)和TOC浓度,并对反硝化、厌氧氨氧化菌群丰度变化和反硝化菌多样性进行分析。结果表明:淀粉浓度在150 mg·L-1时,NO-2-N和NO-3-N的去除率最高,分别达到98.90%和99.86%；NH+4-N去除率在淀粉浓度为50 mg·L-1时最高,为35.98%。随着淀粉浓度的增加,反硝化菌的丰度明显增加,但有机碳对厌氧氨氧化菌群具有抑制作用。当淀粉浓度为150 mg·L-1时,反硝化菌的丰度最大、多样性水平最高、物种数目最大,反硝化细菌优势菌属为未分类的变形菌属和β-变形菌属。
关键词: 有机碳/
沉积物/
反硝化/
厌氧氨氧化/
去除率/
丰度
Abstract:Denitrification has an important effect on alleviating the pollution of the substrate nitrogen,the denitrification and anaerobic ammonia oxidation bacteria can transform the combined nitrogen into nitrogen in sediments of aquaculture pond, which effectively reduces the pollution and the organic carbon has an important role in the process.Effects of organic carbon on denitrification and anaerobic ammonia oxidation in sediments of aquaculture pond were studied,such as physic-chemical analysis and molecular biology analysis methods are used to control different starch concentrations,and the sediments pond are used for substrate,artificial water is used for nutrient solution.The concentrations of NO-2-N,NO-3-N,NH+4-N and TOC of 120 h in the substrates, the abundance of denitrification and anaerobic ammonia oxidation bacteria and the diversity of denitrification bacteria were analyzed.The results showed that the removal efficiency of NO-2-N and NO-3-N was the highest in the 150 mg·L-1 starch, reaching 98.90% and 99.86%, respectively. The removal rate of NH+4-N was the highest in the starch concentration of 50 mg·L-1,was 35.98%.With the increase of starch concentration, the abundance of denitrification bacteria increased markedly,but organic carbon has inhibitory effect on anaerobic ammonia oxidation bacteria. When starch concentration was 150 mg·L-1,the largest abundance of denitrifying bacteria,the highest diversity level of denitrifying bacteria,the largest species number of denitrifying bacteria,and denitrifying bacteria dominant genera for unclassified Proteobacteria and Betaproteobacteria genus.
Key words:organic carbon/
sediments/
denitrification/
anaerobic ammonia oxidation/
removal efficiency/
abundance.
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有机碳对养殖池塘沉积物中反硝化、厌氧氨氧化的影响
高美云1,2,,刘兴国2,
曾宪磊2,
陆诗敏2
1.上海海洋大学水产与生命学院,上海 201306
2.中国水产科学研究院渔业机械仪器研究所,上海 200092
基金项目: 现代农业产业技术体系建设专项(CARS-46) 国家自然科学基金资助项目(31372570) 948节水高效全循环池塘养殖关键技术合作研究(2016-X32)
关键词: 有机碳/
沉积物/
反硝化/
厌氧氨氧化/
去除率/
丰度
摘要:养殖沉积物中反硝化作用对于缓解氮污染有重要的作用,沉积物中的反硝化和厌氧氨氧化菌可将化合态氮转变为氮气,从而有效降低污染,有机碳在该过程中有着重要的作用。为了解有机碳对养殖池塘沉积物中反硝化、厌氧氨氧化的影响,采取理化分析和分子生物学分析等方法,以养殖池塘沉积物为基质、人工配水为营养液,添加不同浓度的淀粉,分析120 h内底物亚硝氮(NO-2-N)、硝氮(NO-3-N)、氨氮(NH+4-N)和TOC浓度,并对反硝化、厌氧氨氧化菌群丰度变化和反硝化菌多样性进行分析。结果表明:淀粉浓度在150 mg·L-1时,NO-2-N和NO-3-N的去除率最高,分别达到98.90%和99.86%；NH+4-N去除率在淀粉浓度为50 mg·L-1时最高,为35.98%。随着淀粉浓度的增加,反硝化菌的丰度明显增加,但有机碳对厌氧氨氧化菌群具有抑制作用。当淀粉浓度为150 mg·L-1时,反硝化菌的丰度最大、多样性水平最高、物种数目最大,反硝化细菌优势菌属为未分类的变形菌属和β-变形菌属。
English Abstract
Effects of organic carbon for denitrification and anaerobic ammonium oxidation in sediments of aquaculture pond
GAO Meiyun1,2,,LIU Xingguo2,
ZENG Xianlei2,
LU Shimin2
1.College of Fisheries and Life Science, Shanghai Ocean University,Shanghai 201306, China
2.Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200092, China
Keywords: organic carbon/
sediments/
denitrification/
anaerobic ammonia oxidation/
removal efficiency/
abundance
Abstract:Denitrification has an important effect on alleviating the pollution of the substrate nitrogen,the denitrification and anaerobic ammonia oxidation bacteria can transform the combined nitrogen into nitrogen in sediments of aquaculture pond, which effectively reduces the pollution and the organic carbon has an important role in the process.Effects of organic carbon on denitrification and anaerobic ammonia oxidation in sediments of aquaculture pond were studied,such as physic-chemical analysis and molecular biology analysis methods are used to control different starch concentrations,and the sediments pond are used for substrate,artificial water is used for nutrient solution.The concentrations of NO-2-N,NO-3-N,NH+4-N and TOC of 120 h in the substrates, the abundance of denitrification and anaerobic ammonia oxidation bacteria and the diversity of denitrification bacteria were analyzed.The results showed that the removal efficiency of NO-2-N and NO-3-N was the highest in the 150 mg·L-1 starch, reaching 98.90% and 99.86%, respectively. The removal rate of NH+4-N was the highest in the starch concentration of 50 mg·L-1,was 35.98%.With the increase of starch concentration, the abundance of denitrification bacteria increased markedly,but organic carbon has inhibitory effect on anaerobic ammonia oxidation bacteria. When starch concentration was 150 mg·L-1,the largest abundance of denitrifying bacteria,the highest diversity level of denitrifying bacteria,the largest species number of denitrifying bacteria,and denitrifying bacteria dominant genera for unclassified Proteobacteria and Betaproteobacteria genus.
