王牧笛1,,,
段俊3,
郑丽明1,
吕正梅1
1. 安徽医科大学基础医学院组织胚胎学教研室, 合肥 230032;
2. 安徽医科大学生命科学学院, 合肥 230032;
3. 中国科学院合肥物质科学研究院安徽光学精密机械研究所, 中国科学院环境光学与技术重点实验室, 合肥 230031
作者简介: 卫旭东(1998-),男,学士,研究方向为大气颗粒物的生殖毒性研究,E-mail:hao0551tian@163.com.
通讯作者: 王牧笛,wangmudi@ahmu.edu.cn ;
基金项目: 安徽医科大学校科学研究基金资助项目（2019xkj008）；安徽医科大学博士科研资助基金（XJ201923）；国家重点研发计划资助项目（2016YFC0201000）中图分类号: X171.5
Reproductive Toxicity of Fine Particulate Matters and Its Mechanism
Wei Xudong1,2,Wang Mudi1,,,
Duan Jun3,
Zheng Liming1,
Lv Zhengmei1
1. Department of Histology and Embryology, School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China;
2. College of Life Sciences, Anhui Medical University, Hefei 230032, China;
3. Anhui Institute of Optics and Fine Mechanics, Hefei Institute of Material Science, Chinese Academy of Sciences, Key Laboratory of Environmental Optics and Technology of Chinese Academy of Sciences, Hefei 230031, China
Corresponding author: Wang Mudi,wangmudi@ahmu.edu.cn ;
CLC number: X171.5
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摘要:大气污染已经成为中国最主要的污染问题。大气细颗粒物作为大气污染的主要成分，具有生殖毒性。本文总结了大气细颗粒物生殖毒性的流行病调查及其动物实验结果，并主要从氧化应激、DNA损伤、炎症反应和血睾屏障的完整性破坏等方面，归纳了近年来大气细颗粒物生殖毒性作用机制的研究成果，对现有研究中存在的问题进行了探讨，这有助于深入推进大气细颗粒物的生殖毒性研究。
关键词: 大气细颗粒物/
PM2.5/
生殖毒性/
人类健康效应/
作用机制
Abstract:The air pollution has become the main pollution problem of China. As the main component of air pollution, fine particulate matters have been shown by many studies to have toxic effects on reproductive system. This paper summarizes the epidemiological investigation and animal experiment results on the reproductive toxicity of fine particulate matters, which mainly focus on the reproductive toxicity of fine particulate matters mediated by oxidative stress, DNA damage, inflammatory response and the disruption of the integrity of blood-testis barrier. This review also discusses the existing problems of current study in order to help to promote the research on reproductive toxic effects of fine particulate matters.
Key words:fine particulate matter/
PM2.5/
reproductive toxicity/
health effects/
mechanisms.
| 孙广权, 杨慧妮, 刘小春, 等. 我国PM2.5主要组分及健康危害特征研究进展[J]. 环保科技, 2015, 21(1):54-59Sun G Q, Yang H N, Liu X C, et al. PM2.5 in China:Chemical compositions and health effects[J]. Environmental Protection and Technology, 2015, 21(1):54-59(in Chinese) |
| Dicker D, Nguyen G, Abate D, et al. Global, regional, and national age-sex-specific mortality and life expectancy, 1950-2017:A systematic analysis for the Global Burden of Disease Study 2017[J]. Lancet, 2018, 392(10159):1684-1735 |
| 李继忠, 边毓尧, 郭文有, 等. PM2.5与呼吸系统疾病发病率关系流行病学调查研究[J]. 陕西医学杂志, 2018, 47(6):805-808Li J Z, Bian Y Y, Guo W Y, et al. Epidemiological survey on the incidence of respiratory diseases in high PM2.5 area[J]. Shaanxi Medical Journal, 2018, 47(6):805-808(in Chinese) |
| 高丽云, 聂林坤, 李潇, 等. 大气细颗粒物PM2.5对心血管疾病损害机制的研究进展[J]. 新乡医学院学报, 2018, 35(7):551-553 |
| Woodward N C, Pakbin P, Saffari A, et al. Traffic-related air pollution impact on mouse brain accelerates myelin and neuritic aging changes with specificity for CA1 neurons[J]. Neurobiology of Aging, 2017, 53:48-58 |
| Ibironke O, Carranza C, Sarkar S, et al. Urban air pollution particulates suppress human T-cell responses to Mycobacterium tuberculosis[J]. International Journal of Environmental Research and Public Health, 2019, 16(21):4112 |
| 胡富宇, 郭婷婷, 朱珊珊, 等. 台州市大气PM2.5对成年男性精液质量的影响[J]. 预防医学, 2019, 31(1):20-22, 27 Hu F Y, Guo T T, Zhu S S, et al. Atmospheric PM2.5 exposure on semen quality in adult men in Taizhou[J]. Preventive Medicine, 2019, 31(1):20-22, 27(in Chinese) |
| Guan Q Q, Chen S Y, Wang B Q, et al. Effects of particulate matter exposure on semen quality:A retrospective cohort study[J]. Ecotoxicology and Environmental Safety, 2020, 193:110319 |
| Santi D, Magnani E, Michelangeli M, et al. Seasonal variation of semen parameters correlates with environmental temperature and air pollution:A big data analysis over 6 years[J]. Environmental Pollution, 2018, 235:806-813 |
| Lao X Q, Zhang Z L, Lau A K H, et al. Exposure to ambient fine particulate matter and semen quality in Taiwan[J]. Occupational and Environmental Medicine, 2018, 75(2):148-154 |
| 吴黎, 张海, 彭哲, 等. 大气细颗粒物长期暴露对男性精子质量的影响[J]. 公共卫生与预防医学, 2018, 29(6):13-16Wu L, Zhang H, Peng Z, et al. Association between long-term exposure to ambient fine particulate matter and semen quality[J]. Journal of Public Health and Preventive Medicine, 2018, 29(6):13-16(in Chinese) |
| Radwan M, Dziewirska E, Radwan P, et al. Air pollution and human sperm sex ratio[J]. American Journal of Men's Health, 2018, 12(4):907-912 |
| Huang X J, Zhang B, Wu L, et al. Association of exposure to ambient fine particulate matter constituents with semen quality among men attending a fertility center in China[J]. Environmental Science & Technology, 2019, 53(10):5957-5965 |
| Zhou N Y, Jiang C T, Chen Q, et al. Exposures to atmospheric PM10 and PM10-2.5 affect male semen quality:Results of MARHCS study[J]. Environmental Science & Technology, 2018, 52(3):1571-1581 |
| Yang S P, Tan Y F, Mei H, et al. Ambient air pollution the risk of stillbirth:A prospective birth cohort study in Wuhan, China[J]. International Journal of Hygiene and Environmental Health, 2018, 221(3):502-509 |
| Green R, Sarovar V, Malig B, et al. Association of stillbirth with ambient air pollution in a California cohort study[J]. American Journal of Epidemiology, 2015, 181(11):874-882 |
| Arroyo V, Díaz J, Carmona R, et al. Impact of air pollution and temperature on adverse birth outcomes:Madrid, 2001-2009[J]. Environmental Pollution, 2016, 218:1154-1161 |
| Liu X, Ye Y F, Chen Y, et al. Effects of prenatal exposure to air particulate matter on the risk of preterm birth and roles of maternal and cord blood LINE-1 methylation:A birth cohort study in Guangzhou, China[J]. Environment International, 2019, 133(Pt A):105177 |
| Stieb D M, Chen L, Beckerman B S, et al. Associations of pregnancy outcomes and PM2.5 in a national Canadian study[J]. Environmental Health Perspectives, 2016, 124(2):243-249 |
| Melody S M, Ford J, Wills K, et al. Maternal exposure to fine particulate matter from a coal mine fire and birth outcomes in Victoria, Australia[J]. Environment International, 2019, 127:233-242 |
| Liang Z J, Yang Y, Qian Z M, et al. Ambient PM2.5 and birth outcomes:Estimating the association and attributable risk using a birth cohort study in nine Chinese cities[J]. Environment International, 2019, 126:329-335 |
| Dadvand P, Rankin J, Rushton S, et al. Ambient air pollution and congenital heart disease:A register-based study[J]. Environmental Research, 2011, 111(3):435-441 |
| Agay-Shay K, Friger M, Linn S, et al. Air pollution and congenital heart defects[J]. Environmental Research, 2013, 124:28-34 |
| Saenen N D, Plusquin M, Bijnens E, et al. In utero fine particle air pollution and placental expression of genes in the brain-derived neurotrophic factor signaling pathway:An ENVIRONAGE birth cohort study[J]. Environmental Health Perspectives, 2015, 123(8):834-840 |
| 潘晓, 邓美洲, 刘琳, 等. 大气PM2.5对人卵巢颗粒细胞的毒性作用及氧化损伤研究[J]. 环境与健康杂志, 2018, 35(3):206-209Pan X, Deng M Z, Liu L, et al. Atmospheric PM2.5-induced toxicity and oxidative damage to human ovarian granulosa cells[J]. Journal of Environment and Health, 2018, 35(3):206-209(in Chinese) |
| Clemens T, Turner S, Dibben C. Maternal exposure to ambient air pollution and fetal growth in North-East Scotland:A population-based study using routine ultrasound scans[J]. Environment International, 2017, 107:216-226 |
| Zang H Y, Cheng H, Song W Y, et al. Ambient air pollution and the risk of stillbirth:A population-based prospective birth cohort study in the coastal area of China[J]. Environmental Science and Pollution Research International, 2019, 26(7):6717-6724 |
| Li S S, Wang H Y, Hu H T, et al. Effect of ambient air pollution on premature SGA in Changzhou City, 2013-2016:A retrospective study[J]. BMC Public Health, 2019, 19(1):705 |
| Zhu J M, Lee R W, Twum C, et al. Exposure to ambient PM2.5 during pregnancy and preterm birth in metropolitan areas of the state of Georgia[J]. Environmental Science and Pollution Research, 2019, 26(3):2492-2500 |
| Yorifuji T, Kashima S, Diez M H, et al. Prenatal exposure to outdoor air pollution and child behavioral problems at school age in Japan[J]. Environment International, 2017, 99:192-198 |
| 周逢海, 周川, 吕海迪, 等. 细颗粒物对雄性小鼠生殖功能的影响[J]. 海南医学, 2019, 30(5):545-548Zhou F H, Zhou C, Lv H D, et al. Effect of fine particles on reproductive function of male mice[J]. Hainan Medical Journal, 2019, 30(5):545-548(in Chinese) |
| Yang Y Y, Yang T T, Liu S X, et al. Concentrated ambient PM2.5 exposure affects mice sperm quality and testosterone biosynthesis[J]. PeerJ, 2019, 7:e8109 |
| Qiu L L, Chen M J, Wang X K, et al. Exposure to concentrated ambient PM2.5 compromises spermatogenesis in a mouse model:Role of suppression of hypothalamus-pituitary-gonads axis[J]. Toxicological Sciences, 2018, 162(1):318-326 |
| 严超, 曹希宁, 沈炼桔, 等. 汽车尾气来源PM2.5颗粒物长期暴露导致SD雄鼠生殖功能损害[J]. 中华男科学杂志, 2016, 22(2):104-109Yan C, Cao X N, Shen L J, et al. Long-term exposure to PM2.5 from automobile exhaust results in reproductive dysfunction in male rats[J]. National Journal of Andrology, 2016, 22(2):104-109(in Chinese) |
| Liao B Q, Liu C B, Xie S J, et al. Effects of fine particulate matter (PM2.5) on ovarian function and embryo quality in mice[J]. Environment International, 2020, 135:105338 |
| 张丰泉, 董恩恒, 王茂, 等. PM2.5对雌鼠生殖内分泌水平和妊娠结局的影响[J]. 中国实验动物学报, 2017, 25(4):455-460Zhang F Q, Dong E H, Wang M, et al. Effects of PM2.5 on reproductive hormone levels and pregnancy outcome in female rats[J]. Acta Laboratorium Animalis Scientia Sinica, 2017, 25(4):455-460(in Chinese) |
| 张丰泉, 赵杉, 董恩恒, 等. 核转录因子NF-E2相关因子2信号通路在PM2.5致雌性大鼠生殖损伤中的作用[J]. 中国实验动物学报, 2018, 26(5):639-643Zhang F Q, Zhao S, Dong E H, et al. Effect of NF-E2 related factor 2 pathway activation on PM2.5 induced reproductive injury in female rats[J]. Acta Laboratorium Animalis Scientia Sinica, 2018, 26(5):639-643(in Chinese) |
| 张丰泉, 董恩恒, 薛玉雪. PM2.5短期暴露对大鼠子宫组织的损伤及其作用机制[J]. 中国实验动物学报, 2019, 27(6):740-746Zhang F Q, Dong E H, Xue Y X. Effect and mechanism of short-term exposure to PM2.5 on the rat uterine injury[J]. Acta Laboratorium Animalis Scientia Sinica, 2019, 27(6):740-746(in Chinese) |
| 李久存, 魏永杰, 陈田, 等. 妊娠期和哺乳期暴露于大气颗粒物对仔鼠中枢神经系统的影响[J]. 环境与健康杂志, 2011, 28(5):377-379Li J C, Wei Y J, Chen T, et al. Effects of maternal exposure to atmospheric particulates during pregnancy and lactation period on central nervous system of filial generation among rats[J]. Journal of Environment and Health, 2011, 28(5):377-379(in Chinese) |
| Klocke C, Allen J L, Sobolewski M, et al. Neuropathological consequences of gestational exposure to concentrated ambient fine and ultrafine particles in the mouse[J]. Toxicological Sciences, 2017, 156(2):492-508 |
| Klocke C, Allen J L, Sobolewski M, et al. Exposure to fine and ultrafine particulate matter during gestation alters postnatal oligodendrocyte maturation, proliferation capacity, and myelination[J]. NeuroToxicology, 2018, 65:196-206 |
| 王婷婷, 郑昕蕊, 李文燕, 等. 母源性PM2.5暴露致子代鼠大脑皮层神经炎症与星形胶质细胞和小胶质细胞激活[J]. 中国组织化学与细胞化学杂志, 2018, 27(4):313-320Wang T T, Zheng X R, Li W Y, et al. Maternal PM2.5 exposure induces the activation of astrocytes and microglia and subsequent neuroinflammation in the cerebral cortex in mouse offspring[J]. Chinese Journal of Histochemistry and Cytochemistry, 2018, 27(4):313-320(in Chinese) |
| Ren L H, Huang J, Wei J L, et al. Maternal exposure to fine particle matters cause autophagy via UPR-mediated PI3K-mTOR pathway in testicular tissue of adult male mice in offspring[J]. Ecotoxicology and Environmental Safety, 2020, 189:109943 |
| 郑昕蕊, 赵会, 田换兵, 等. 妊娠期PM2.5暴露影响子代鼠主要脏器发育[J]. 中国组织化学与细胞化学杂志, 2017, 26(4):366-372Zheng X R, Zhao H, Tian H B, et al. PM2.5 exposure during pregnancy causes abnormal development of major organs in offspring mice[J]. Chinese Journal of Histochemistry and Cytochemistry, 2017, 26(4):366-372(in Chinese) |
| 巩艳, 李文燕, 郑昕蕊, 等. 妊娠期大气细颗粒物暴露对新生小鼠肝脏发育的影响[J]. 中国组织化学与细胞化学杂志, 2016, 25(5):406-410Gong Y, Li W Y, Zheng X R, et al. Effects of gestational exposure to atmospheric fine particulate matter on liver development of newborn mice[J]. Chinese Journal of Histochemistry and Cytochemistry, 2016, 25(5):406-410(in Chinese) |
| 林彬彬. 大鼠妊娠前及妊娠期福州地区PM2.5暴露对子代肾发育及肾脏功能的远期影响[D]. 福州:福建医科大学, 2016:22-35 Lin B B. Prenatal and perinatal exposure to ambient PM2.5 of Fuzhou on the renal development and long-term function[D]. Fuzhou:Fujian Medical University, 2016:22-35(in Chinese) |
| 汤雯婷, 匡丽云, 龚景进, 等. 母源性细颗粒物暴露对仔鼠生长发育及肺功能的影响[J]. 中华围产医学杂志, 2016, 19(2):108-113 |
| de Barros Mendes Lopes T, Groth E E, Veras M, et al. Pre- and postnatal exposure of mice to concentrated urban PM2.5 decreases the number of alveoli and leads to altered lung function at an early stage of life[J]. Environmental Pollution, 2018, 241:511-520 |
| 洪新如, 刘超斌, 黄惠娟, 等. 环境大气细颗粒物宫内暴露对大鼠子代免疫功能相关指标的影响[J]. 中华围产医学杂志, 2018, 21(2):94-101 |
| Rhee J, Han E, Nam K J, et al. Assessment of hair cell damage and developmental toxicity after fine particulate matter 2.5μm (PM2.5) exposure using zebrafish (Danio rerio) models[J]. International Journal of Pediatric Otorhinolaryngology, 2019, 126:109611 |
| 吴德生, 王宏辉, 袁建辉, 等. 细颗粒物对斑马鱼胚胎发育的影响[J]. 癌变·畸变·突变, 2018, 30(4):307-309, 314 Wu D S, Wang H H, Yuan J H, et al. Effect of fine particle exposure on development of zebrafish embryos[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2018, 30(4):307-309, 314(in Chinese) |
| 吴德生, 杨飞, 袁建辉, 等. PM2.5对斑马鱼胚胎发育早期氧化应激和DNA甲基化调控相关基因表达的影响[J]. 癌变·畸变·突变, 2017, 29(5):379-382Wu D S, Yang F, Yuan J H, et al. Effect of PM2.5 on expression of oxidative stress and DNA methylation genes during early development of zebrafish embryos[J]. Carcinogenesis, Teratogenesis & Mutagenesis, 2017, 29(5):379-382(in Chinese) |
| Zhang Y, Li S Y, Li J J, et al. Developmental toxicity induced by PM2.5 through endoplasmic reticulum stress and autophagy pathway in zebrafish embryos[J]. Chemosphere, 2018, 197:611-621 |
| 王云彪, 侯晓丽, 武海涛, 等. PM2.5对秀丽隐杆线虫的毒性效应[J]. 城市环境与城市生态, 2012, 25(6):10-13Wang Y B, Hou X L, Wu H T, et al. Toxicological exposure effect test of using C. elegans as a model organism[J]. Urban Environment snd Urban Ecology, 2012, 25(6):10-13(in Chinese) |
| Sun L M, Lin Z Q, Liao K, et al. Adverse effects of coal combustion related fine particulate matter (PM2.5) on nematode Caenorhabditis elegans[J]. Science of the Total Environment, 2015, 512-513:251-260 |
| Zhao Y L, Lin Z Q, Jia R H, et al. Transgenerational effects of traffic-related fine particulate matter (PM2.5) on nematode Caenorhabditis elegans[J]. Journal of Hazardous Materials, 2014, 274:106-114 |
| Wang M D, Nie Y G, Liu Y, et al. Transgenerational effects of diesel particulate matter on Caenorhabditis elegans through maternal and multigenerational exposure[J]. Ecotoxicology and Environmental Safety, 2019, 170:635-643 |
| Ebisu K, Bell M L. Airborne PM2.5 chemical components and low birth weight in the northeastern and mid-Atlantic regions of the United States[J]. Environmental Health Perspectives, 2012, 120(12):1746-1752 |
| Sun X L, Luo X P, Zhao C M, et al. The associations between birth weight and exposure to fine particulate matter (PM2.5) and its chemical constituents during pregnancy:A meta-analysis[J]. Environmental Pollution, 2016, 211:38-47 |
| Bell M L, Belanger K, Ebisu K, et al. Prenatal exposure to fine particulate matter and birth weight[J]. Epidemiology, 2010, 21(6):884-891 |
| 王芳, 李岩, 卢昕烁, 等. PM2.5对血管内皮细胞氧化应激和凋亡的影响及机制[J]. 中国病理生理杂志, 2017, 33(3):423-427Wang F, Li Y, Lu X S, et al. Oxidative stress and apoptosis in endothelial cells exposed to PM2.5[J]. Chinese Journal of Pathophysiology, 2017, 33(3):423-427(in Chinese) |
| 商学军, 叶章群, 熊承良, 等. 活性氧致人精子运动功能和存活力变化的分析[J]. 中华男科学, 2003, 9(6):439-442Shang X J, Ye Z Q, Xiong C L, et al. Analysis of the changes of movement function and viability in human spermatozoa induced by reactive oxygen species[J]. Acto Andrologica Sinica, 2003, 9(6):439-442(in Chinese) |
| 陈霞. 活性氧在卵泡发育及胚胎发育中的作用[J]. 实用医学杂志, 2008, 24(11):2014-2015 |
| de Melo J O, Soto S F, Katayama I A, et al. Inhalation of fine particulate matter during pregnancy increased IL-4 cytokine levels in the fetal portion of the placenta[J]. Toxicology Letters, 2015, 232(2):475-480 |
| Zhou L X, Su X, Li B H, et al. PM2.5 exposure impairs sperm quality through testicular damage dependent on NALP3 inflammasome and miR-183/96/182 cluster targeting FOXO1 in mouse[J]. Ecotoxicology and Environmental Safety, 2019, 169:551-563 |
| 黄佳伟, 鲁娴娴, 崔海燕, 等. PM2.5通过NF-κB/NLRP3途径促进金黄色葡萄球菌肺炎的研究[J]. 生态毒理学报, 2019, 14(5):133-143Huang J W, Lu X X, Cui H Y, et al. Staphylococcus aureus pneumonia aggravation by PM2.5 via NF-κB/NLRP3 pathway[J]. Asian Journal of Ecotoxicology, 2019, 14(5):133-143(in Chinese) |
| 邹杨, 李淑珍, 李新鸣, 等. PM2.5通过ROS-Nrf2/NF-κB信号通路诱导人支气管上皮细胞炎症反应[J]. 中国公共卫生, 2020, 36(10):1438-1442Zou Y, Li S Z, Li X M, et al. PM2.5 induces inflammation in human bronchial epithelial cells through ROS-Nrf2 signaling pathway[J]. Chinese Journal of Public Health, 2020, 36(10):1438-1442(in Chinese) |
| 颜森波, 于国伟, 谌慧. PM2.5对DNA的损伤作用概述[J]. 职业与健康, 2015, 31(6):847-850Yan S B, Yu G W, Chen H. An overview on effect of PM2.5 on DNA damage[J]. Occupation and Health, 2015, 31(6):847-850(in Chinese) |
| Arlt V M, Zhan L, Schmeiser H H, et al. DNA adducts and mutagenic specificity of the ubiquitous environmental pollutant 3-nitrobenzanthrone in Muta Mouse[J]. Environmental and Molecular Mutagenesis, 2004, 43(3):186-195 |
| Topinka J, Rossner P Jr, Milcova A Jr, et al. DNA adducts and oxidative DNA damage induced by organic extracts from PM2.5 in an acellular assay[J]. Toxicology Letters, 2011, 202(3):186-192 |
| 张琳, 牛静萍, 徐佳, 等. 大气细颗粒物PM2.5对大鼠睾丸组织细胞周期的影响[J]. 生态毒理学报, 2009, 4(2):271-275Zhang L, Niu J P, Xu J, et al. Effects of PM2.5 on the cell cycle of testis spermatogenic cells in rats[J]. Asian Journal of Ecotoxicology, 2009, 4(2):271-275(in Chinese) |
| Scull C M, Tabas I. Mechanisms of ER stress-induced apoptosis in atherosclerosis[J]. Arteriosclerosis, Thrombosis, and Vascular Biology, 2011, 31(12):2792-2797 |
| Liu X N, Jin X T, Su R J, et al. The reproductive toxicology of male SD rats after PM2.5 exposure mediated by the stimulation of endoplasmic reticulum stress[J]. Chemosphere, 2017, 189:547-555 |
| Cao X N, Shen L J, Wu S D, et al. Urban fine particulate matter exposure causes male reproductive injury through destroying blood-testis barrier (BTB) integrity[J]. Toxicology Letters, 2017, 266:1-12 |
| Liu B, Shen L J, Zhao T X, et al. Automobile exhaust-derived PM2.5 induces blood-testis barrier damage through ROS-MAPK-Nrf2 pathway in Sertoli cells of rats[J]. Ecotoxicology and Environmental Safety, 2020, 189:110053 |
| Liu J H, Ren L H, Wei J L, et al. Fine particle matter disrupts the blood-testis barrier by activating TGF-β3/p38 MAPK pathway and decreasing testosterone secretion in rat[J]. Environmental Toxicology, 2018, 33(7):711-719 |
| Oh S. Disturbance in testosterone production in leydig cells by polycyclic aromatic hydrocarbons[J]. Development & Reproduciton, 2014, 18(4):187-195 |
| O'Donnell L, McLachlan R I, Wreford N G, et al. Testosterone withdrawal promotes stage-specific detachment of round spermatids from the rat seminiferous epithelium[J]. Biology of Reproduction, 1996, 55(4):895-901 |
| Ramaswamy S, Weinbauer G F. Endocrine control of spermatogenesis:Role of FSH and LH/testosterone[J]. Spermatogenesis, 2014, 4(2):e996025 |
| Corradi P F, Corradi R B, Greene L W. Physiology of the hypothalamic pituitary gonadal axis in the male[J]. The Urologic Clinics of North America, 2016, 43(2):151-162 |
