刘浩然¹,
王俊霞^1,2,4,,,
沈晓芳¹,
顾海东³,
张占恩^1,2,4
1. 苏州科技大学环境科学与工程学院, 苏州 215009;
2. 苏州科技大学, 江苏省环境科学与工程重点实验室, 苏州 215009;
3. 新加坡国立大学苏州研究院, 苏州 215024;
4. 苏州科技大学, 水处理技术与材料协同创新中心, 苏州 215009

作者简介: 刘浩然(1999-),男,硕士研究生,研究方向为土壤POPs复合污染生物修复,E-mail:1015931826@qq.com.

通讯作者: 王俊霞,wjx_7297@163.com ;

基金项目: 苏州市科技计划项目（SNG 2016012，SNG 2018050）


中图分类号: X171.5

Seed Germination and Seedling Growth of Soya Bean and Cucumber under PCBs and PAHs Stress

Liu Haoran¹,
Wang Junxia^1,2,4,,,
Shen Xiaofang¹,
Gu Haidong³,
Zhang Zhanen^1,2,4
1. School of Environmental Science and Technology, Suzhou University of Science and Technology, Suzhou 215009, China;
2. Key Laboratory of Environmental Science and Engineering of Jiangsu Province, Suzhou University of Science and Technology, Suzhou 215009, China;
3. NUS(Suzhou) Research Institute, Suzhou 215024, China;
4. Water Treatment Technology and Materials Collaborative Innovation Center, Suzhou University of Science and Technology, Suzhou 215009, China

Corresponding author: Wang Junxia,wjx_7297@163.com ;

CLC number: X171.5

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摘要:多氯联苯（PCBs）和多环芳烃（PAHs）是2类持久性有机污染物，其对生态健康和食品安全的威胁日益受到关注。以大豆（Glycine max（Linn.）Merr.）和黄瓜（Cucumis sativus L.）为研究对象，在PCB28、菲单一和复合胁迫下，考察种子发芽、幼苗生长等特征，测定PCB28和菲在植物体内的含量。2，4，4'-三氯联苯（PCB28）、菲单一及复合胁迫8 d后，高浓度组大豆和黄瓜种子相对发芽率不高于80%，幼苗湿重减少率高达90%，叶绿素荧光参数中最大光化学效率（F_v/F_m）显著下降，这表明，高浓度PCB28和菲对植物的生长和光合能力都产生明显的毒害作用。在中、高浓度的PCB28（0.1 mg·L^-1和1.0 mg·L^-1）胁迫下，大豆和黄瓜可以通过抗逆境反应减少对PCB28的吸收。大豆和黄瓜幼苗不同部位中PCB28和菲的含量存在根 > 茎 > 叶的规律。研究结果可为探究PCBs、PAHs的单一及复合污染的植物毒性研究提供基础数据。
关键词: 多氯联苯/
2,4,4'-三氯联苯/
菲/
大豆/
黄瓜/
发芽/
光合特性/
体内分布

Abstract:As persistent organic pollutants, polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) raise great concern due to their ecological health risk and food safety threat. Herein, we utilized the soya bean (Glycine max (Linn.) Merr.) and cucumber (Cucumis sativus L.) seeds as target organisms to study the effects of PCBs and PAHs on plant growth and germination under the conditions of single or joint stress. Under the stress of 2,4,4'-trichlorobiphenyl (PCB28) or phenanthrene as well as their combined action for 8 d, compared to the control group, the relative germination rate of soya bean and cucumber in the high concentration treatment group was not greater than 80%, while the relative wet weight decrease rate of their seedlings reached up to 90%; also, the maximum photochemical efficiency (F_v/F_m) was significantly reduced. These data confirm that the high concentration of PCB28 and phenanthrene had prominently inhibitory effects on the photosynthetic capacity and growth of plants. Under the stress of medium and high concentration of PCB28 (0.1 mg L^-1and 1.0 mg L^-1), plants could decrease the PCB28 absorption through their anti-stress responses. Under the stress of PCB28 and phenanthrene for 8 d, the residues of two pollutants in different tissues of soya bean and cucumber seedlings followed the order as root > stem > leaf. These findings pave the way for the further investigation on the phytotoxicity of PCBs and PAHs.
Key words:polychlorinated biphenyls/
2,4,4'-trichlorobiphenyl/
phenanthrene/
soya bean/
cucumber/
germination/
photosynthetic characteristics/
tissue distribution.

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