聂芳红¹,
蔡吉榛²,
王小宁²,
林红英²,
Ravi Gooneratne³,
Anthony Hay⁴,
马驿²,
巨向红²,
郑锦庆²,
陈进军^2,,
1. 广东海洋大学食品科技学院, 湛江 524088;
2. 广东海洋大学农学院, 湛江 524088;
3. 林肯大学农业与生命学院, 基督城 7647, 新西兰;
4. 康奈尔大学农业与生命科学学院, 伊萨卡 14853, 美国

作者简介: 聂芳红(1969-),女,硕士,高级实验师,研究方向为食品毒理学,E-mail:15913577717@163.com.

通讯作者: 陈进军,jjchen777@aliyun.com

基金项目: 广东省科技计划国际合作项目(2010B050600004,2016A050502062)


中图分类号: X171.5

EROD Activity and cyp1a mRNA Expression in Zebrafish Embryo Exposed to Marine Sediment DlPCBs Extract

Nie Fanghong¹,
Cai Jizhen²,
Wang Xiaoning²,
Lin Hongying²,
Gooneratne Ravi³,
Anthony Hay⁴,
Ma Yi²,
Ju Xianghong²,
Zheng Jinqing²,
Chen Jinjun^2,,
1. Food Sci-Tech College, Guangdong Ocean University, Zhanjiang 524088, China;
2. Agricultural College, Guangdong Ocean University, Zhanjiang 524088, China;
3. Faculty of Agriculture & Life Sciences, Lincoln University, Christchurch 7647, New Zealand;
4. College of Agriculture and Life Sciences, Cornell University, Ithaca 14853, USA

Corresponding author: Chen Jinjun,jjchen777@aliyun.com

CLC number: X171.5

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摘要:为探究湛江近海域海洋沉积物中类二噁英多氯联苯(dioxin-like polychlorinated biphenyls,DlPCBs)的生物学毒性效应，选取湛江近海域2个地点(近工业区TS和近生活区JSW)采集沉积物样品，制备近海域沉积物DlPCBs提取物，将斑马鱼胚胎暴露于不同浓度的DlPCBs提取物，测定斑马鱼胚胎7-乙氧基异吩恶唑脱乙基酶(ethoxyresorufin-O-deethylase,EROD)活性和cyp1a mRNA相对表达量。结果发现，JSW采样点DlPCBs提取物染毒组EROD酶活性变化与TS采样点DlPCBs提取物一致，在各染毒浓度下，斑马鱼仔鱼EROD酶活性为对照组的1.1～1.8倍。TS和JSW采样点不同浓度DlPCBs提取物暴露斑马鱼胚胎96 h后，使斑马鱼仔鱼cyp1a mRNA相对表达量是对照组的3.36～19.45倍。说明一定浓度的近海沉积物DlPCBs能诱导斑马鱼仔鱼EROD酶活性和cyp1a mRNA表达量升高，且呈现浓度－效应关系。
关键词: 类二噁英多氯联苯/
斑马鱼胚胎/
EROD酶/
cyp1a mRNA/
近海沉积物

Abstract:To study the bio-toxicity of dioxin-like polychlorinated biphenyls (DlPCBs) in marine sediment, the sediments from Zhanjiang industrial and living area (TS and JSW) were collected and extracted focusing on DlPCBs, respectively. Then a range of zebrafish embryos were exposed to different concentrations of the sediment DlPCBs extracts to examine the ethoxyresorufin-O-deethylase (EROD) activity and the expression of cyp1a mRNA. The results showed that the DlPCBs extract from TS and JSW sediments had similarity to cause marked induction of EROD in the zebrafish embryos.The EROD activities in zebrafish embryos induced by different concentrations of the DlPCBs extract were 1.1 to 1.8 times higher than that in the control embryos, respectively, and after the zebrafish embryos were exposed to different concentrations of the DlPCBs for 96 h, the juvenile cyp1a mRNA relative expressions were significantly enhanced by 3.36 to 19.45 times of that in the control group, respectively. It is indicated that certain concentrations of the DlPCBs extract increased EROD activity and cyp1a mRNA in zebrafish larvae, with a concentration-effect relationship.
Key words:dioxin-like polychlorinated biphenyls/
zebrafish embryo/
EROD/
cyp1a mRNA/
marine sediment.