高上吉,
钟晓霞,
刘婉莹,
王勉之,
孙静,
李弘程,
孙永学^,
华南农业大学 广东省兽药研制与安全评价重点实验室, 广州 510642

作者简介: 高上吉(1989-),男,硕士研究生,研究方向为兽医药理学与毒理学,E-mail:710648618@qq.com.

通讯作者: 孙永学,sunyx@scau.edu.cn

基金项目: 国家自然科学基金项目(31172368)


中图分类号: X171.5

Comet Assay Study on DNA Damage of Embryonic Cells in Caenorhabditis elegans Induced by Roxarsone

Gao Shangji,
Zhong Xiaoxia,
Liu Wanying,
Wang Mianzhi,
Sun Jing,
Li Hongcheng,
Sun Yongxue^,
Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, China

Corresponding author: Sun Yongxue,sunyx@scau.edu.cn

CLC number: X171.5

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摘要:为评价洛克沙胂的遗传毒性,采用单细胞凝胶电泳(彗星实验),研究了不同浓度的洛克沙胂对秀丽隐杆线虫胚胎细胞脱氧核醣核酸(DNA)的损伤作用。提取秀丽隐杆线虫的胚胎细胞,分别暴露于0(空白对照)、50、250、500 μg·L^-1含洛克沙胂的溶液染毒1 h。用彗尾DNA百分比含量(TDNA%)、彗星尾长(TL)和Olive尾矩(OTM)作为DNA损伤的指标。实验结果表明,与空白对照组比较,处理组中彗尾DNA百分比含量、彗星尾长以及Olive尾矩显著增加(P<0.01)。随着洛克沙胂浓度的增加,彗尾DNA百分比含量、彗星尾长以及Olive尾矩逐渐增加,其相关系数r>0.99,说明在实验浓度范围内,存在极显著的浓度-效应关系。洛克沙胂对秀丽隐杆线虫胚胎细胞DNA具有损伤作用,彗星实验操作简便、快速、灵敏度高,能够反映出洛克沙胂的遗传毒性。因此,通过彗星实验建立实验室检测洛克沙胂遗传毒性的方法具有可行性。
关键词: 洛克沙胂/
秀丽隐杆线虫/
彗星实验/
DNA损伤/
遗传毒性

Abstract:To evaluate the genotoxicity of roxarsone, the present study investigated the damage effects of different concentrations of roxarsone on deoxyribonucleic acid (DNA) of embryonic cells in Caenorhabditis elegans by using the single cell gel-electrophoresis (SCGE). The extracted embryonic cells of Caenorhabditis elegans were exposed to 0 (blank control), 50, 250, 500 μg·L^-1 roxarsone for 1 h, respectively. The percentage of comet tail DNA (TDNA%), the comet tail length (TL) and Olive tail moment (OTM) were used as the indicators of DNA damage. The results showed that, TDNA percentage, TL and OTM in all treatment groups increased significantly compared with these in the control group (P<0.01). TDNA percentage, TL and OTM increased gradually according to the increased concentrations of roxarsone. The correlation coefficient of r greater than 0.99, which indicated that the concentration-effect relationship was observed in the experimental concentration range. Roxarsone had damage effect on DNA of embryonic cells in Caenorhabditis elegans. Comet assay was simple, fast, and highly-sensitive to reflect the genotoxicity of roxarsone. Thus, as a laboratory based method, it is practical to detect the genotoxicity of roxarsone by using comet assay.
Key words:roxarsone/
Caenorhabditis elegans/
comet assay/
DNA damage/
genotoxicity.