余锦波,
邹鉴,
朱雨晴,
朱家力,
杜晶,
李潇潇,
郭晴,
杨旭^,
华中师范大学生命科学学院, 武汉 430079

作者简介: 余锦波(1995-),男,本科生,研究方向为环境毒理学,Email:646488921@qq.com.

通讯作者: 杨旭,yangxu@mail.ccnu.edu.cn

基金项目: 国家自然科学基金重点项目(51136002)


中图分类号: X171.5

An in Vitro Study on Oxidative Stress Induced by H₂O₂ and Protective Effect of GSH in Rat Tracheal Epithelial Cell

Yu Jinbo,
Zou Jian,
Zhu Yuqing,
Zhu Jiali,
Du Jing,
Li Xiaoxiao,
Guo Qing,
Yang Xu^,
School of Life Science, Central China Normal University, Wuhan 430079, China

Corresponding author: Yang Xu,yangxu@mail.ccnu.edu.cn

CLC number: X171.5

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摘要:许多具有氧化作用的空气污染物,均能使细胞产生氧化损伤,使胸腺基质淋巴生成素(thymic stromal lymphopoietin, TSLP)含量上升。而TSLP是一种启动过敏性炎症的重要因子,会导致哮喘等疾病发生率的上升。在本研究中用过氧化氢H₂O₂)模拟具有氧化作用的空气污染物进行染毒,研究细胞氧化应激水平的变化,并讨论还原型谷胱甘肽(GSH)对细胞受氧化损伤的保护作用。将大鼠支气管上皮细胞(RTE)分组培养,每组设置6个平行实验,分别用低、中、高剂量H₂O₂染毒3 h;高剂量设置1个重复,作为保护组,在染毒前用GSH保护2 h。结果显示,高剂量组H₂O₂(3.2 mmol·L^-1)染毒的细胞,其细胞活力下降(P<0.01),丙二醛(MDA)水平上升(P<0.01),TSLP水平上升(P<0.05),与之相比,用GSH保护后的同剂量染毒组,上述指标得到全面缓解(P<0.01)。这表明高浓度的H2O2会损伤细胞活力,并使MDA及TSLP水平上升,而GSH对TSLP及MDA的升高有极显著的抑制作用,即对细胞有一定的保护作用。
关键词: 过氧化氢/
大鼠支气管上皮细胞/
RTE细胞/
谷胱甘肽/
胸腺基质淋巴细胞生成素/
丙二醛/
氧化损伤

Abstract:It has been acknowledged that many oxidative air pollutants can cause the oxidative stress and increase the levels of thymic stromal lymphopoietin (TSLP). TSLP, a kind of important allergic disease initiator, can lead to higher incidence of diseases such as asthma. In this study, H₂O_{2 was used to simulate the oxidative pollutants to stimulate cells, in order to study the changes of oxidative stress level and the protective effects of GSH. The rat tracheal epithelial (RTE) cells were cultured in six parallel test groups, including the low, medium and high dose groups, and a repeat high dose group for the protection effect was treated with GSH for 2 h before the 3 h-H2O2-exposure. The MDA (P<0.01) and TSLP (P<0.05) levels increased while the cell viability were decreased (P<0.01) in the high dose group (3.2 mmol·L^-1). After the pretreatment of GSH, the changes of MDA and TSLP levels, and the cell viability decreased, compared with those of the same dose H2O2 group. It is indicated that high dose H2O2 can damage cell viability and increase the levels of MDA and TSLP, while the GSH can ameliorate the damage and protect the cells.
Key words:H2O2/
rat tracheal epithelial cell/
glutathione (GSH)/
thymic stromal lymphopoietin (TSLP)/
oxidative damage/}