张瑞刚,
仪慧兰^,
山西大学生命科学学院, 太原 030006

作者简介: 张瑞刚(1984-),男,博士,研究方向为环境微生物,E-mail:zhangruigang1224@163.com.

通讯作者: 仪慧兰,yihl@sxu.edu.cn

基金项目: 国家自然科学基金项目(No.30870454,30470318,31371868)；山西省科技攻关计划(No.20120322008-02)；山西省回国留学人员科研项目(No.2012013)


中图分类号: X171.5

ROS and Calcium Signal is Involved in Lead Toxicity in Yeast Cells

Zhang Ruigang,
Yi Huilan^,
School of Life Sciences, Shanxi University, Taiyuan 030006, China

Corresponding author: Yi Huilan,yihl@sxu.edu.cn

CLC number: X171.5

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摘要:以模式生物酵母菌为材料,研究铅对细胞的毒性效应,探讨胞内活性氧(ROS)和Ca²⁺在铅诱导细胞死亡中的作用。结果显示,浓度为5~100 mg·L^-1的硝酸铅可降低酵母细胞活性,诱导酵母细胞死亡,随着铅浓度的提高和作用时间的延长,细胞死亡率增高。在铅处理组酵母细胞中,ROS和Ca²⁺水平显著升高,线粒体膜电位明显下降;用1 mmol·L^-1的外源抗坏血酸(AsA)能降低铅引发的酵母细胞死亡,0.5 mmol·L^-1的钙离子螯合剂乙二醇双四乙酸(EGTA)或0.1 mmol·L^-1的质膜Ca²⁺通道特异性抑制剂氯化镧(LaCl₃)亦可明显抑制铅引起的酵母细胞死亡。研究结果表明,铅诱发的酵母细胞死亡与处理组胞内ROS和Ca²⁺升高有关,高浓度的Ca²⁺可能通过诱导线粒体膜通透性转变孔道开放,或者高水平ROS可能损伤线粒体膜,致线粒体膜电位下降,继而激活相关下游信号导致细胞死亡。
关键词: 铅/
酵母/
细胞毒性/
ROS/
Ca²⁺

Abstract:This paper was conducted to research the cellular toxicity of lead based on the model yeasts to explore the role that intracellular reactive oxygen species (ROS) and Ca²⁺ play in the mechanism of the cell death induced by lead. The results revealed that the cellular bioactivity of yeasts was inhibited by lead nitrate at the concentration from 5 to 100 mg·L^-1and the cell death induced by lead was found. Yeast cell mortality increases with the increase of lead concentration and the extension of time. The levels of ROS and Ca²⁺ elevated significantly and the mitochondrial membrane potential decreased dramatically in yeast cells under lead stress. Cell death induced by lead was decreased by ascorbic acid (AsA) at the concentration of 1 mmol·L^-1 and was inhibited by the calcium chelator (EGTA) at the concentration of 0.5 mmol·L^-1 or the specific inhibitor of plasma membrane Ca²⁺ channel (LaCl₃) at the concentration of 0.1 mmol·L^-1. The results showed that cell death induced by lead was related to the levels of intracellular ROS and Ca²⁺. The opening of mitochondrial membrane permeability transition pore channels may be induced by high levels of Ca²⁺, or mitochondrial membranes were broken by high levels of ROS, which lead to decreasing membrane potential and cause cell death by activating down-stream relevant signal pathways.
Key words:lead/
yeast cell/
cytotoxity/
ROS/
Ca²⁺.