张耘泽,
耿歌,
秦婷婷,
刘德立,
熊丽,
华中师范大学生命科学学院 湖北省遗传调控与整合生物学重点实验室, 武汉 430079
作者简介: 王丽(1992-),女,硕士研究生,研究方向为分子毒理学,E-mail:waw934356@sina.com.
通讯作者: 熊丽,xionglily@mail.ccnu.edu.cn
基金项目: 国家自然科学基金项目(No.31071653，31371893)；中央高校基本科研业务费专项项目(No.CCNU12A02001)中图分类号: X171.5
Variations of Relevant Enzyme Activities and Genes Expressions in Zebrafish under Acute Exposure to Beta Cypermethrin
Wang Li,Zhang Yunze,
Geng Ge,
Qin Tingting,
Liu Deli,
Xiong Li,
Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Science, Central China Normal University, Wuhan 430079, China
Corresponding author: Xiong Li,xionglily@mail.ccnu.edu.cn
CLC number: X171.5
-->
摘要
HTML全文
图
参考文献
相关文章
施引文献
资源附件
访问统计
摘要:高效氯氰菊酯广泛地应用于农业生产及日常生活中的害虫防治,但对水生生物却毒性极高。为探究高效氯氰菊酯对斑马鱼的急性毒性,以总超氧化物歧化酶(总SOD)、过氧化氢酶(CAT)和乙酰胆碱酯酶(AChE)活性以及相关的基因表达量变化为检测指标,研究其对斑马鱼成鱼的影响。急性毒性试验结果显示,高效氯氰菊酯对斑马鱼属剧毒物质,斑马鱼的急性中毒症状为身体蜷曲、抽搐,鳃盖扇动加快,游动能力减弱,间歇性出现无规律急速游动和撞壁行为。酶活测定结果显示,斑马鱼组织中总SOD、CAT和AChE活性与高效氯氰菊酯呈现低浓度诱导、高浓度抑制的剂量效应关系;相关基因表达量的检测结果显示,高效氯氰菊酯会诱导斑马鱼肝脏、肠和脑中Sod1、Cat以及Ache的mRNA表达上调。研究表明,高效氯氰菊酯的神经毒性和氧化损伤共同造成了斑马鱼的中毒甚至死亡。
关键词: 高效氯氰菊酯/
斑马鱼/
急性毒性/
氧化损伤
Abstract:Beta cypermethrin is widely used in agricultural production and pest control in daily life. However, it is highly toxic to aquatic organisms. To explore the acute toxic effects of beta cypermethrin to zebrafish, the activity of total superoxide dismutase (total SOD), catalase (CAT), acetylcholin esterase (AChE), and related gene expression were measured after the exposure of beta cypermethrin. The results of acute tocixity test showed that beta cypermethrin is highly toxic to zebrafish, the symptoms of poisoning include curling, tic, faster flapping of operculum, weaker swimming ability, intermittent appearance of irregular fast moving and striking behaviour. The enzymatic activity of total SOD, CAT, and AChE in zebrafish increased in low dose of beta cypermethrin and decreased in high dose. The results of related gene expression showed that beta cypermethrin up-regulated the mRNA expression of Sod1, Cat, Ache in zebrafish liver, gut, and brain. All the results indicate that the combination effect of neurotoxicity and oxidative damage lead to the toxication even death of zebrafish.
Key words:beta cypermethrin/
zebrafish/
acute tocixity/
oxidative damage.
