阎希柱1,2,,,
宿丽丽1,2
1. 集美大学, 厦门 361000;
2. 农业部东海海水健康养殖重点实验室, 厦门 361000
作者简介: 陈西(1991-),女,硕士,研究方向为水域生态毒理学,E-mail:1361158525@qq.com.
通讯作者: 阎希柱,xzyan@jmu.edu.cn ;
基金项目: 海洋公益性行业科研专项(201205009-4)中图分类号: X171.5
Effects of PFOS on the Genes Transcription and Enzymes Activity of CYPs, GST in Polychaete Perinereis nuntia
Chen Xi1,2,Yan Xizhu1,2,,,
Su Lili1,2
1. Jimei University, Xiamen 361021, China;
2. Key Laboratory of Healthy Mariculture for the East China Sea, Ministry of Agriculture, P. R. China, Xiamen 361021, China;
Corresponding author: Yan Xizhu,xzyan@jmu.edu.cn ;
CLC number: X171.5
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摘要:多毛类沙蚕已经广泛应用于海洋环境污染的生物监测,但其对新型持久性有机污染物全氟辛烷磺酰基化合物PFOS的毒理学研究尚无报道。本研究以潮间带优势种多齿围沙蚕(Perinereis nuntia)为研究对象,以细胞色素P450(CYP)、谷胱甘肽硫转移酶(GST)的基因和酶作为联合指标,研究了在PFOS亚致死浓度(4、8、16 mg·L-1)暴露第1、4、7、14天及净水恢复5 d后多齿围沙蚕CYP431A1、CYP424A1基因转录水平和EROD酶活性、GST omega基因转录水平和GST酶活性的响应情况。结果表明,PFOS暴露对EROD的抑制具有明显的时间-效应关系;CYP2系成员CYP431A1基因转录水平对PFOS的响应具有良好的剂量-效应关系并在胁迫第14天表现出最高的可诱导性;CYP4系基因CYP424A1的转录在4、8 mg·L-1处理组中与PFOS暴露时间正相关。II相解毒系统成员GST酶活和GST omega基因的响应均表现出随着PFOS胁迫时间的延长先下降,后上升的规律;多齿围沙蚕在高强度PFOS胁迫下仍可加速新陈代谢,表现出对PFOS的耐受性;净水恢复阶段,各指标都有向对照组水平恢复的趋势。总之,基因和蛋白的响应表明CYPs和GST在多齿围沙蚕PFOS新陈代谢中发挥重要作用,这些基因和酶具有作为生物标志物监测海洋潮间带PFOS污染效应的潜力。
关键词: 全氟辛烷磺酸化合物(PFOS)/
多齿围沙蚕/
氧化损伤/
细胞色素P450/
谷胱甘肽硫转移酶
Abstract:Polychaete has been widely used to monitor the environmental pollution. Perfluorooctane sulfonate (PFOS) is an emerging persistent organic pollutant that has global distribution. However, the effect of PFOS on the dominant species of polychaete, nereis, has received little attention. To research its potential biomarkers for PFOS pollution, this paper studied the combine response of genes transcription and enzymes activity of cytochrome P450 (CYP), glutathione S-transferase (GST) in nereis, Perinereis nuntia. The sandworms were exposed to sublethal concentrations of PFOS (4, 8, 16 mg·L-1) for 1, 4, 7, 14 days and recovered for 5 days, respectively. The PFOS-induced inhibition of EROD showed a time-dependent relationship clearly. The P. nuntia CYP431A1 (CYP2 clan) mRNA was sensitively expressed to PFOS exposure with a clear dose-dependent relationship and showed the highest inducibility on day 14. The transcription levels of CYP424A1, a CYP4 clan gene, in 4 and 8 mg·L-1 treatments were all positively correlated with the PFOS exposure time. As one of phase II detoxification enzymes, the GST enzyme and GST omega gene both decreased first and upregulated then with the extension of PFOS exposure. P. nuntia could still accelerate metabolism under the serious exposure and showed its tolerance for PFOS. During recovery period, all the observed indexes showed a tendency to recover to the control level. In conclusion, the response of gene transcription and enzyme activity showed that CYPs and GST of P. nuntia played important roles in the PFOS detoxification and these indexes of P. nuntia would have great potentials as biomarkers to detect the PFOS pollution in marine intertidal zone.
Key words:PFOS/
Perinereis nuntia/
oxidative stress/
cytochrome P450/
glutathione S-transferase.
