周遵春2,,,
王摆2,,,
高杉2,
孙红娟2,
关晓燕2,
董颖2,
闫喜武1
1. 大连海洋大学, 大连 116023;
2. 辽宁省海洋水产科学研究院, 大连 116023
作者简介: 潘泳嘉(1988-),男,硕士研究生,研究方向为动物遗传育种与繁殖,E-mail:820092256@qq.com.
通讯作者: 周遵春,wangbai1980@hotmail.com ; 王摆,zunchunz@hotmail.com ;
基金项目: 海洋公益性行业科研专项(201205012-7)；大连市科学技术基金(2012J21DW029)中图分类号: X171.5
Analysis of Differentially Expressed Genes in the Tube Feet of Sea Cucumber (Apostichopus japonicus) Co-exposed to BTEXs by Transcriptome Sequencing
Pan Yongjia1,2,Zhou Zunchun2,,,
Wang Bai2,,,
Gao Shan2,
Sun Hongjuan2,
Guan Xiaoyan2,
Dong Ying2,
Yan Xiwu1
1. Dalian Ocean University, Dalian 116023, China;
2. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China
Corresponding authors: Zhou Zunchun,wangbai1980@hotmail.com ; Wang Bai,zunchunz@hotmail.com ;
CLC number: X171.5
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摘要:为了分析苯系物(BTEXs)联合暴露后仿刺参(Apostichopus japonicus)管足转录组差异表达基因,采用Illumina HiSeqTM 2000测序技术,对1.0 mg·L-1苯系物(B)联合暴露12 h后和对照组(C)的仿刺参管足组织分别进行转录组测序。经Trinity软件进行de novo组装,获得了145 675条unigenes。利用公共数据库进行同源比对,共注释了35 330条unigenes。对比分析苯系物联合暴露组和对照组的转录组测序结果,获得了2 418个差异表达基因(DEGs) (|Log2 Fold changes| ≥ 1且FDR ≤ 0.001),其中,上调表达和下调表达基因分别为1 049和1 369个。GOseq分析结果显示,158个DEGs显著富集在149个GO terms中,包括103个生物学过程、17个细胞组分和29个分子功能(P < 0.05);KEGG代谢通路分析结果显示994个差异基因映射到268条代谢通路,这些差异表达基因参与的生理过程与其他生物的同源基因参与的信号传导、癌症、外源性化合物的生物降解代谢等过程相类似。上述结果为在转录组水平筛选苯系物的生物标志物,解析苯系物对仿刺参毒性作用的分子机制提供了科学参考。
关键词: 苯系物/
仿刺参/
管足/
差异表达基因/
转录组测序
Abstract:In order to identify and analyze the differentially expressed genes (DEGs), transcriptome sequencing in the tube feet of sea cucumber Apostichopus japonicus exposed to 0.0 (C), 1.0 mg·L-1 BTEXs (B) for 12 h, was performed respectively using the Illumina HiSeqTM 2000 platform. The clean reads were then de novo assembled into 145 675 unigenes, and 35 330 unigenes were annotated by a similarity search against the public databases. By comparing B and C using the criteria |Log2 Fold changes| ≥ 1 and false discovery rate (FDR) ≤ 0.001, 2 418 DEGs were identified, among which 1 049 were up-regulated and 1 369 were down-regulated. GOseq analysis revealed that for B vs. C, 158 DEGs were highly enriched in 149 GO terms, including 103 biological processes, 17 cellular components and 29 molecular function terms (P value < 0.05). Pathways associated with BTEXs challenge were also mined. The result indicated that 994 DEGs were enriched in 268 pathways, including signal transduction, cancer, xenobiotics biodegradation and metabolism pathways. The results obtained in this study could be used for the screening of BTEXSs biomarkers on the transcriptome level, and provide the reference to understand the molecular mechanism of toxic effects of BTEXs on sea cucumber A. japonicus.
Key words:BTEXs/
Apostichopus japonicus/
tube feet/
differentially expressed gene/
transcriptome sequencing.
