Meng-QiuDong,Ph.D.
AssistantInvestigator,NIBS,Beijing,ChinaPhone：010-80726688-8515
Fax：010-80706053
E-mail：dongmengqiu@nibs.ac.cn
教育经历Education2001年耶鲁大学分子生物物理学与生物化学系博士Ph.D.,DepartmentofMolecularBiophysics&Biochemistry,YaleUniversity,USA
1995年中国科学院上海生物化学研究所硕士M.Sc.,ShanghaiInstituteofBiochemistry,ChineseAcademyofSciences,China
1992年四川大学生物系学士B.Sc.,DepartmentofBiology,SichuanUniversity,China
工作经历ProfessionalExperience2007年-北京生命科学研究所研究员AssistantInvestigator,NationalInstituteofBiologicalSciences,Beijing,China
2003-2007年美国斯克利普斯研究院博士后PostdoctoralResearchAssociate,TheScrippsResearchInstitute,USA
2001-2003年美国加洲大学圣地亚哥分校医学院博士后Postdoctoralresearcher,UCSDSchoolofMedicine,USA
研究概述ResearchDescription本实验室的研究主题包含生物学和质谱技术两部分，二者相辅相成，共同发展。在生物学研究方面，我们以线虫为模式生物研究衰老过程及其调控机制。我们对线虫自然衰老过程中亚细胞器（如线粒体）和分子水平（转录组和蛋白质组）的变化进行分析，比较长寿突变体线虫与野生型线虫之间有何不同。在衰老的调节机制的研究中，我们主要关注胰岛素信号通路。在质谱方面，我们致力于基于质谱的蛋白质组学技术的应用与开发，主要包括肽序列从头测序的算法开发、基于电子转移解离（ETD）谱图的高效蛋白质鉴定技术、和通过鉴定交联肽段来研究蛋白－蛋白相互作用的方法。实验室目前共拥有三台质谱仪，包括一台装备了ETD的LTQ-Orbitrap，一台HCTultraPTM，以及一台TSQDiscovery。发表文章SelectedPublications:1Tie-MeiLi,JieChen,XiangkeLi,Xiao-JunDing,YaoWu,Li-FengZhao,SheChen,XiaoguangLei*,andMeng-QiuDong*.(2013)AbsoluteQuantificationofaSteroidHormonethatRegulatesDevelopmentinCaenorhabditiselegansAnal.Chem.,85(19),pp9281–9287*correspondingauthors.
2TaoL,XieQ,DingYH,LiST,PengS,ZhangYP,TanD,YuanZ,DongMQ*(2013)CAMKIIandCalcineurinregulatethelifespanofCaenorhabditiselegansthroughtheFOXOtranscriptionfactorDAF-16,eLIFE,2:e00518
3DingYH,DuYG,LuoS,LiYX,LiTM,YoshinaS,WangX,KlageK,MitaniS,YeK*,DongMQ*(2013)CharacterizationofPUD-1andPUD-2,TwoProteinsUp-RegulatedinaLong-Liveddaf-2Mutant.PLoSONE.8(6):e67158*correspondingauthors
4BingYang#,Yan-JieWu#,MingZhu#,Sheng-BoFan,JinzhongLin,KunZhang,ShuangLi,HaoChi,Yu-XinLi,Hai-FengChen,Shu-KunLuo,Yue-HeDing,Le-HengWang,ZhiqiHao,Li-YunXiu,SheChen,KeqiongYe,Si-MinHe*andMeng-QiuDong*(2012)Identificationofcross-linkedpeptidesfromcomplexsamples.NatureMethod.9:904-906.doi:10.1038/nmeth.2099
5SunRX*,DongMQ*,SongCQ,ChiH,YangB,XiuLY,TaoL,JingZY,LiuC,WangLH,FuY,HeSM(2010)ImprovedPeptideIdentificationforProteomicAnalysisBasedonComprehensiveCharacterizationofElectronTransferDissociationSpectra.JProteomeRes.9(12):6354-67.*correspondingauthors.
6ChiH,SunRX,YangB,SongCQ,WangLH,LiuC,FuY,YuanZF,WangHP,HeSM*,DongMQ*(2010)pNovo:DenovoPeptideSequencingandIdentificationUsingHCDSpectra.JofProteomeRes.9(5):2713-24*correspondingauthors.
7FekairiS,ScaglioneS,ChahwanC,TaylorER,TissierA,CoulonS,DongMQ,RuseC,YatesJRIII,RussellP,FuchsRP,McGowanCH,GaillardPL(2009)HumanSLX4IsaHollidayJunctionResolvaseSubunitthatBindsMultipleDNARepair/RecombinationEndonucleases.Cell138(1):78-89
8ZhangDW,ShaoJ,LinJ,ZhangN,LuBJ,LinSC,DongMQ,HanJ.(2009)RIP3,anEnergyMetabolismRegulatorthatSwitchesTNF-InducedCellDeathfromApoptosistoNecrosis.Science325(5938):332-6
9DongMQ,VenableJD,AuN,XuT,ParkSK,CociorvaD,JohnsonJ,DillinA,andYatesJR,III(2007)QuantitativeMassSpectrometryIdentifiesInsulinSignalingTargetsinC.elegans.Science317:660-3
10McClatchyDB*,DongMQ*,WuCC*,Venable,JD,YatesJR,III(2007)15NMetabolicLabelingofMammalianTissueswithSlowProteinTurnover.JProteomeRes.6(5);2005-10*Theseauthorscontributedequally.
11SunP,NewL,MoserBA,LiY,YoshizukaN,LiaoR,XieC,DengQ,YamoutM,DongMQ,YatesJR,III,WrightPE,HanJ,ChenJ,FrangouCG.(2007)PRAKMediatesaNovelPathwayEssentialforOncogenicras-InducedSenescenceandTumorSuppression.Cell128(2):295-308
12VarsanoT*,DongMQ*,NiesmanI*,GaculaH,LouX,MaT,TestaJR,YatesJR,III,andFarquharMG.(2006)GIPCisrecruitedbyAPPLtoperipheralTrkAendosomesandregulatesTrkAtraffickingandsignaling.Mol.Cell.Biol.26(23):8942-52*Theseauthorscontributedequally.
13VenableJD,DongMQ,WohlschlegelJ,DillinA,andYatesJR,III.(2004)Automatedapproachforquantitativeanalysisofcomplexpeptidemixturesfromtandemmassspectra.NatureMethods1(1):39-45.
14DongMQ,ChaseD,PatikoglouGA,KoelleMR.(2000)MultipleRGSproteinsalterneuralGproteinsignalingtoallowC.eleganstorapidlychangebehaviorwhenfed.GenesDev.14:2003-14.
