金香淑博士北京生命科学研究所研究员
XiangshuJin,Ph.D.
AssistantInvestigator,NIBS,Beijing，China
Phone：86-10-80726688
Fax：86-10-80726689
E-mail：jinxiangshu@nibs.ac.cn
教育经历Education2002年美国密歇根州立大学化学博士
Ph.D.,Chemistry,MichiganStateUniversity,EastLansing,MI,USA
1995年北京大学化学学士
B.S.,Chemistry,PekingUniversity,Beijing,China
工作经历ProfessionalExperience2012-北京生命科学研究所研究员
AssistantInvestigator,NationalInstituteofBiologicalSciences,Beijing,China
2007-2012年美国霍华德休斯医学研究所/哥伦比亚大学生物化学与分子生物物理系副研究员
ResearchSpecialist,HowardHughesMedicalInstitute;AdjunctAssociateResearchScientist,DepartmentofBiochemistryandMolecularBiophysics,ColumbiaUniversity,NewYork,NY,USA
2006-2007年美国哥伦比亚大学生物化学与分子生物物理系副研究员
AssociateResearchScientist,DepartmentofBiochemistryandMolecularBiophysics,ColumbiaUniversity,NewYork,NY,USA
2003-2006年美国哈佛大学分子与细胞生物学系博士后
PostdoctoralResearchFellow,DepartmentofMolecularandCellularBiology,HarvardUniversity,Cambridge,MA,USA
2003年美国密歇根州立大学生物化学与分子生物学系博士后
PostdoctoralResearchScientist,DepartmentofBiochemistry&MolecularBiology,MichiganStateUniversity,EastLansing,MI,USA
1995-1998年北京大学考古文博学院助教
Assistant,ConservationLaboratory,SchoolofArchaeologyandMuseology,PekingUniversity,Beijing,China
研究概述ResearchDescription我们实验室的研究兴趣在于生物模式形成(biologicalpatternformation)所必需的细胞间通讯的分子机制。在具有特定功能的各种生物模式的形成过程中，细胞表面受体与相邻细胞表达的配体分子特异性地相互作用。表面受体将这些细胞外的分子信号跨膜转导至细胞内，从而启动细胞内信号转导的级联反应，以达到相邻细胞之间功能上的相互协调。我们综合运用结构生物学、生物物理、生物化学、细胞生物学、计算生物学等多种手段，在原子和分子水平上探索有关受体与配体之间特异性相互作用的机理，并延伸到细胞乃至系统水平上研究和阐明各种生物模式的形成机制。实验室目前的研究主要集中在平面细胞极性(planarcellpolarity)建立的分子机制，我们将着力研究CELSR亚家族的粘附G蛋白偶联受体及其相关粘附受体所介导的细胞间相互作用的分子机制、受体的激活机制、以及下游分子信号通路。Ourlaboratoryisinterestedinunderstandingthemolecular“languages”ofcell-cellcommunicationthatcoordinatesbiologicalpatternformation.Formationofmulticellularpatternsassociatedwithspecificbiologicalfunctionsentailsintricatecommunicationnetworkswherebycellstalktoeachotherthroughspecificinteractionsbetweensurfacereceptorsandligandspresentedfromneighboringcells.Theseextracellularmolecularcuesarethentransducedintoappropriateintracellularresponses,whichallowagroupofcellstocoordinatetheiractivitiesandcollectivelyformelaboratemulticellularpatterns.Weuseamultidisciplinaryapproachcombiningstructuralbiology,biophysics,biochemistry,cellbiology,andcomputationalbiologytostudythemechanismsofbiologicalpatternformationattheatomic,molecular,cellular,andsystemslevels.Ourcurrenteffortsarefocusedonunderstandingthemolecularinteractionsthatunderlietheestablishmentofplanarpolarity,aformofmulticellularorganizationthatcoordinatespolarizationofcellswithinatissueplaneandiscriticalfordiversedevelopmentalprocesses.Tothisend,wetakeamultiprongedapproachtostudytheextracellularinteractionsmediatedbytheCELSRfamilyadhesionGPCRsandrelatedadhesionreceptors,theactivationmechanismsofthesereceptors,andtheirdownstreamsignalingpathways.发表文章PublicationsPeer-reviewedresearcharticles:1.CaoY*,PanY*,HuangH*,JinX,LevinEJ,KlossB,ZhouM(2013)GatingoftheTrkHionchannelbyitsassociatedRCKproteinTrkA.Nature496,317-322.(*equalcontribution)2.HarrisonOJ,VendomeJ,BraschJ,JinX,HongS,KatsambaPS,AhlsenG,TroyanovskyRB,TroyanovskySM,HonigB,ShapiroL(2012)Nectinectodomainstructuresrevealacanonicaladhesiveinterface.NatureStructuralandMolecularBiology906-915.3.GlaaserIW,OsteenJD,PuckerinA,SampsonKJ,JinX,KassRS(2012)PerturbationofsodiumchannelstructurebyaninheritedlongQTSyndromemutation.NatureCommunications3,706.4.JinX*,WalkerMA*,FelsovalyiK*,VendomeJ*,BahnaF,MannepalliS,CosmanescuF,AhlsenG,HonigB,ShaprioL(2012)CrystalstructuresofDrosophilaN-cadherinectodomainregionsrevealawidelyusedclassofCa²⁺-freeinterdomainlinkers.Proc.Natl.Acad.Sci.U.S.A.109,E127-134.(*equalcontribution)

5.VendomeJ*,PosyS*,JinX,BahnaF,AhlsenG,ShapiroL,HonigB(2011)Moleculardesignprinciplesunderlyingβ-strandswappingintheadhesivedimerizationofcadherins.NatureStructuralandMolecularBiology18,693-700.(*equalcontribution)6.CaoY,JinX*,LevinEJ*,HuangH*,ZongY*,QuickM,WengJ,PanY,LoveJ,PuntaM,RostB,HendricksonWA,JavitchJA,ZhouM(2011)Crystalstructureofaphosphorylation-coupledsaccharidetransporter.Nature473,50-54.(*equalcontribution)7.CaoY*,JinX*,HuangH*,DerebeMG,LevinEJ,KabaleeswaranV,PanY,PuntaM,LoveJ,WengJ,QuickM,YeS,KlossB,BruniR,Martinez-HackertE,HendricksonWA,RostB,JavitchJA,RajashankarKR,JiangY,ZhouM(2011)CrystalstructureofapotassiumiontransporterTrkH.Nature471,336-340.(*equalcontribution)8.HarrisonOJ*,JinX*,HongS,BahnaF,AhlsenG,BraschJ,WuY,VendomeJ,FelsovalyiK,HamptonCM,TroyanovskyRB,Ben-ShaulA,FrankJ,TroyanovskySM,ShapiroL,HonigB(2011)TheextracellulararchitectureofadherensjunctionsrevealedbycrystalstructuresoftypeIcadherins.Structure19,244-256.(*equalcontribution)9.WuY,JinX,HarrisonO,ShapiroL,HonigBH,Ben-ShaulA.(2010)Cooperativitybetweentransandcisinteractionsincadherin-mediatedjunctionformation.Proc.Natl.Acad.Sci.U.S.A.107,17592-17597.10.KoehnkeJ*,KatsambaPS*,AhlsenG,BahnaF,VendomeJ,HonigB,ShapiroL,JinX*(2010)Spliceformdependenceofbeta-neurexin/neuroliginbindinginteractions.Neuron67,61-74.(*equalcontribution)11.HarrisonOJ,BahnaF,KatsambaPS,JinX,BraschJ,VendomeJ,AhlsenG,CarrollKJ,PriceSR,HonigB,ShapiroL(2010)Two-stepadhesivebindingbyclassicalcadherins.NatureStructuralandMolecularBiology17,348-357.12.CiattoC,BahnaF,ZampieriN,VanSteenhouseHC,KatsambaPS,AhlsenG,HarrisonOJ,BraschJ,JinX,PosyS,VendomeJ,RanschtB,JessellTM,HonigB,ShapiroL(2010)T-cadherinstructuresrevealanoveladhesivebindingmechanism.NatureStructuralandMolecularBiology17,339-347.13.KoehnkeJ,JinX,TrbovicN,KatsambaPS,BraschJ,AhlsenG,ScheiffeleP,HonigB,PalmerAG3rd,ShapiroL(2008)Crystalstructuresofβ-neurexin1andβ-neurexin2ectodomainsanddynamicsofspliceinsertionsequence4.Structure16,410-421.14.KoehnkeJ*,JinX*,BudreckEC,PosyS,ScheiffeleP,HonigB,ShapiroL(2008)Crystalstructureoftheextracellularcholinesterase-likedomainfromneuroligin-2.Proc.Natl.Acad.Sci.U.S.A.105,1873-1878.(*equalcontribution)15.JinX,TownleyR,ShapiroL(2007)StructuralinsightintoAMPKregulation:ADPcomesintoplay.Structure15,1285-1295.16.LishkoPV*,ProckoE*,JinX*,PhelpsCB,GaudetR(2007)TheankyrinrepeatsofTRPV1bindmultipleligandsandmodulatechannelsensitivity.Neuron54,905-918.(*equalcontribution)17.JinX,TouheyJ,GaudetR(2006)StructureoftheN-terminalankyrinrepeatdomainoftheTRPV2ionchannel.J.Biol.Chem.281,25006-25010.18.BejarCM,JinX,BallicoraMA,PreissJ(2006)Moleculararchitectureoftheglucose1-phosphatesiteinADP-glucosepyrophosphorylases.J.Biol.Chem.281,40473-40484.19.JinX,BallicoraMA,PreissJ,GeigerJH(2005)CrystalstructureofpotatotuberADP-glucosepyrophosphorylase.EMBOJ.24,694-704.20.JinX,FoleyKM,GeigerJH(2004)Thestructureofthe1L-myo-inositol1-phosphatesynthase/NAD⁺/2-deoxy-D-glucitol6-(E)-vinylhomophosphonatecomplexdemandsarevisionoftheenzyme’smechanism.J.Biol.Chem.279,13889-13895.21.JinX,GeigerJH(2003)StructuresofNAD⁺-andNADH-bound1-L-myo-inositol1-phosphatesynthase.Acta.Crystallogr.Sect.D:Biol.Crystallogr.59,1154-1164.Invitedreviewsandbookchapters:1.RohsR*,JinX*,WestSM,JoshiR,HonigB,MannRS(2010)Originsofspecificityinprotein-DNArecognition.Annu.Rev.Biochem.79,233-269.(*co-firstauthors)2.GeigerJH,JinX(2006)Thestructureandmechanismofmyo-inositol-1-phosphatesynthase.Subcell.Biochem.39,157-180.