细胞培养肉规模化生产工艺及反应器展望

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李雪良¹，张国强¹，赵鑫锐¹，孙秀兰²，周景文¹，堵国成¹，陈坚^1*

1. 江南大学生物工程学院，江苏无锡 2141222. 江南大学食品学院，江苏无锡 214122

收稿日期:2019-04-15修回日期:2019-05-22出版日期:2020-01-22发布日期:2020-01-14
通讯作者:陈坚

Prospects of process and bioreactors for large scale cultured meat production

Xueliang LI¹, Guoqiang ZHANG¹, Xinrui ZHAO¹, Xiulan SUN², Jingwen ZHOU¹, Guocheng DU¹, Jian CHEN^1*

1. School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China 2. School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, China

Received:2019-04-15Revised:2019-05-22Online:2020-01-22Published:2020-01-14

摘要/Abstract

摘要： 体外培养动物肌肉组织作为食用材料的构想在20世纪30年代就有了，90年代末还出现了相关的专利，但该技术到目前为止还没有大规模生产的例子。过去五、六年来，尤其是2013年世界首个细胞培养牛肉汉堡公开试吃活动以后，培养肉(Cultured meat)被大量报道。截至2019年3月，全世界已有超过25家公司宣布正在研究将培养肉推向市场，但仍没有一家企业能把产品放到普通消费者面前。这其中一部分限制因素是监管和社会伦理方面的争议，但更大的问题是生产成本居高不下。规模化生产是降低单位成本的有效手段，但目前业界对动物细胞大规模培养的传质、传热、混合、剪切应力等问题还缺乏足够关注。动物细胞培养传统上多用于量低价高的医疗、医药领域，提高产量的途径往往是多组体积不超过2?20 m3的设备并列运行，保持培养环境均一性问题不大。但人工培养动物肌肉细胞若作为大宗食品进入市场与传统养殖业进行有效竞争，所需的生物反应器规模和细胞密度还需在目前常用的动物细胞培养技术基础上提高至少一个数量级。因此，培养肉大规模培养工艺与设备的进步是使其成为替代性动物蛋白来源的前提条件。本工作对培养肉规模化生产能采用的反应器类型、操作模式、存在的主要问题及与反应器相关的研究方向进行探讨，为国内相关企业和科研机构提供参考。

引用本文

李雪良张国强赵鑫锐孙秀兰周景文堵国成陈坚. 细胞培养肉规模化生产工艺及反应器展望[J]. 过程工程学报, 2020, 20(1): 3-11.
Xueliang LI Guoqiang ZHANG Xinrui ZHAO Xiulan SUN Jingwen ZHOU Guocheng DU Jian CHEN . Prospects of process and bioreactors for large scale cultured meat production[J]. Chin. J. Process Eng., 2020, 20(1): 3-11.

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