新型耐碱蛋白A介质的制备与性能

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韦巍^1,2，黄永东^2*，赵岚²，吴学星²，朱天孝¹，李冬雪¹，靳海波¹，张荣月^1*，苏志国²，马光辉²

1. 北京石油化工学院化学工程学院，北京 1026172. 中国科学院过程工程研究所生化工程国家重点实验室，北京 100190

收稿日期:2018-07-17修回日期:2018-09-30出版日期:2019-06-22发布日期:2019-06-20
通讯作者:黄永东

基金资助:国家重点研发计划资助项目;中国科学院重点部署项目;北京市自然科学基金资助项目;北京市教委科技一般项目;北京市属高校高水平教师队伍建设支持计划高水平创新团队建设计划项目

Preparation and properties of a new alkali-resistant rProtein A chromatographic medium

Wei WEI^1,2, Yongdong HUANG^2*, Lan ZHAO², Xuexing WU², Tianxiao ZHU¹, Dongxue LI¹, Haibo JIN¹, Rongyue ZHANG^1*, Zhiguo SU², Guanghui MA²

1. Department of Chemical Engineering, Beijing Institute of Petrochemical Technology, Beijing 102617, China2. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China

Received:2018-07-17Revised:2018-09-30Online:2019-06-22Published:2019-06-20
Contact:HUANG YongDong

摘要/Abstract

摘要： 以蛋白A结构中耐碱性更好的C区为基础，构建一种新型耐碱蛋白A，并偶联至琼脂糖基质，获得新型耐碱蛋白A亲和层析介质，分别用激光共聚焦显微镜和石英晶体微天平研究了人免疫球蛋白(hIgG)与蛋白A介质的结合过程。结果表明，该介质具有较商品配基更高的结合力和稳定的再生性能，hIgG动态载量达62.0 mg/mL，40次再生操作后载量为初始载量的84%。该介质在抗体纯化领域具有较好的应用前景。

引用本文

韦巍黄永东赵岚吴学星朱天孝李冬雪靳海波张荣月苏志国马光辉. 新型耐碱蛋白A介质的制备与性能[J]. 过程工程学报, 2019, 19(3): 609-616.
Wei WEI, Yongdong HUANG Lan ZHAO Xuexing WU Tianxiao ZHU Dongxue LI Haibo JIN Rongyue ZHANG Zhiguo SU Guanghui MA. Preparation and properties of a new alkali-resistant rProtein A chromatographic medium[J]. Chin. J. Process Eng., 2019, 19(3): 609-616.

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