从雨生红球藻藻泥中选择性提取虾青素

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任晓丽^1,2，陈林^2*，李润植³，刘天中²

1. 中国海洋大学食品科学与工程学院，山东青岛 2660032. 中国科学院青岛生物能源与过程研究所生物燃料重点实验室，山东青岛 2661013. 山西农业大学农学院，山西晋中 030801

收稿日期:2017-12-28修回日期:2018-07-06出版日期:2019-02-22发布日期:2019-02-12
通讯作者:陈林

基金资助:微藻燃油关键技术研发与中试;经济微藻转化煤基二氧化碳联产高值生物产品关键技术研发

Selectively extract astaxanthin from wet biomass of Haematococcus pluvialis

Xiaoli REN^1,2, Lin CHEN^2*, Runzhi LI³, Tianzhong LIU²

1. College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China2. Key Laboratory of Biofuel, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China3. College of Agriculture, Shanxi Agricultural University, Jinzhong, Shanxi 030801, China

Received:2017-12-28Revised:2018-07-06Online:2019-02-22Published:2019-02-12

摘要/Abstract

摘要： 以雨生红球藻湿藻泥为原料，研究了不同有机溶剂对胞内油脂和虾青素选择性提取分离的影响，通过酸解破壁提高虾青素和油脂的提取效率。结果表明，连续乙醇提取可对胞内色素和油脂有效分级提取，先提取出极性组分(叶绿素和极性脂)，再提取中性组分(类胡萝卜素和中性脂)。中等极性溶剂或溶剂体系对类胡萝卜素的选择性和提取率较好；乙醇/乙酸乙酯混合溶剂提取类胡萝卜素的总得率(干重)达25.31 mg/g，提取率为69.35%。对雨生红球藻湿细胞进行酸解破壁处理有助于提高虾青素和油脂的提取率。在最优酸解破壁条件(盐酸浓度1 mol/L，温度60℃，时间60 min)下，含水80%的雨生红球藻藻泥的油脂总得率(干重)达418 mg/g，总脂提取率达97%。

引用本文

任晓丽陈林李润植刘天中. 从雨生红球藻藻泥中选择性提取虾青素[J]. 过程工程学报, 2019, 19(1): 136-143.
Xiaoli REN Lin CHEN Runzhi LI Tianzhong LIU. Selectively extract astaxanthin from wet biomass of Haematococcus pluvialis[J]. Chin. J. Process Eng., 2019, 19(1): 136-143.

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