刘琪1,
曾昭海2,
胡跃高2,
高志强1,,
1.山西农业大学农学院 太谷 030801
2.中国农业大学农学院 北京 100193
基金项目: 山西省自然科学面上青年基金项目201701D221181
山西省回国留学人员科研项目2016-068
国家现代农业产业技术体系专项资金CARS-03-01-24
详细信息
作者简介:乔月静, 主要从事土壤微生态群落研究。E-mail:qyjsxau@126.com
通讯作者:高志强, 主要从事作物栽培与耕作研究。E-mail:gaozhiqiang1964@126.com
中图分类号:S344.13计量
文章访问数:948
HTML全文浏览量:0
PDF下载量:526
被引次数:0
出版历程
收稿日期:2018-06-04
录用日期:2018-09-23
刊出日期:2019-01-01
Effect of rotation on nematode community diversity in rhizosphere soils and yield of sweet potato
QIAO Yuejing1,,LIU Qi1,
ZENG Zhaohai2,
HU Yuegao2,
GAO Zhiqiang1,,
1. College of Agronomy, Shanxi Agricultural University, Taigu 030801, China
2. College of Agronomy, China Agricultural University, Beijing 100193, China
Funds: the Shanxi Provincial Natural Science Foundation for Youths201701D221181
Shanxi Scholarship Council of China2016-068
the Special Fund for the Industrial System Construction of Modern Agriculture of ChinaCARS-03-01-24
More Information
Corresponding author:GAO Zhiqiang, E-mail:gaozhiqiang1964@126.com
摘要
HTML全文
图
参考文献
相关文章
施引文献
资源附件
访问统计
摘要
摘要:甘薯茎线虫（Ditylenchus destructor）是国际检疫植物寄生线虫，甘薯茎线虫病是危害我国甘薯生产的严重病害之一。本文通过不同种植方式下甘薯根际土壤线虫群落结构的变化，探索轮作对甘薯茎线虫病防治的作用，明确变性梯度凝胶电泳（DGGE）在土壤线虫群落研究上应用的可行性。试验在河北省卢龙县多年连作的甘薯地上进行，种植方式分别为：A1，休闲→甘薯；A2，玉米-冬闲→甘薯；A3，玉米-黑麦→甘薯；A4，大豆-冬闲→甘薯；A5，大豆-黑麦→甘薯；CK，甘薯连作。提取3个时期甘薯根际土壤线虫，扩增线虫ITS区序列，采用DGGE技术分析土壤线虫群落。结果表明，与连作相比，轮作方式显著提高甘薯根际土壤线虫群落多样性，降低根际土壤甘薯茎线虫数量（P < 0.05），冬季轮作黑麦（A3，A5）使甘薯根际土壤线虫群落结构更加稳定，随时间变化幅度小。甘薯根际土壤中检测到的线虫优势属有：茎线虫属、矛线虫属、滑刃线虫属、头叶线虫属、短体线虫属、小环线虫属、刺线虫属、真滑刃线虫属、双胃线虫属。轮作方式提高甘薯产量42.08%~55.83%，降低病情指数22.72%~30.79%，不同轮作方式之间对甘薯产量和病情的影响差异不显著，收获期的甘薯茎线虫数量与甘薯产量和病情指数显著相关（P < 0.05）。因此，轮作方式能够显著提高甘薯根际土壤线虫群落多样性和甘薯产量，DGGE可有效检测土壤线虫群落；大豆-黑麦→甘薯是经济效益和生态效益较好的轮作措施。
Abstract:Sweet potato rot nematode (Ditylenchus destructor) is a severe disease that can cause significant loss of sweet potato yield and that can destroy biotic community diversity in rhizosphere soils. In this study, nematode community structure in rhizosphere soils under sweet potato were investigated to verify the impact of rotation cropping patterns on rot nematode disease and the feasibility of denatured gradient gel electrophoresis (DGGE) in soil nematode research. The experiment was conducted on a long-term continuous sweet potato field in Lulong County, Hebei Province in 2014 to (→) 2015, where sweet potato rot nematode disease was seriously epidemic. The cropping patterns were included A1 (fallow→sweet potato), A2 (maize-fallow→sweet potato) A3 (maize-rye→sweet potato), A4 (bean-fallow→sweet potato), A5 (bean-rye→sweet potato) and CK (continuous sweet potato cropping). The nematodes were separately extracted from sweet potato rhizosphere soil in May, July and September in 2015, and the ITS genes analyzed using PCR-DGGE. The results showed that compared with continuous cropping, crop rotation significantly increased the diversity of nematode community, and decreased the population of sweet potato rot nematode in rhizosphere soil (P < 0.05). Community structure of nematodes in rhizosphere soil under sweet potato was stabilized by winter rotation with rye (A3 and A5). Based on PCR-DGGE, 9 genera of nematodes were detected in the rhizosphere soil of sweet potato-Ditylenchus, Dorylaimus, Aphelenchoides, Cephalobus, Pratylenchus, Criconemella, Belonolaimus, Aphelenchus, and Diplogasterida. While Ditylenchus was the dominant genus in all the cropping patterns, Dorylaimus, Aphelenchoides and Cephalobus were the main genera. Crop rotation increased sweet potato yield by 42.08%-55.83% and decreased disease index by 22.72%-30.79%. However, different crop rotations had no significant difference on sweet potato yield and disease index. The population of Ditylenchus destructor was significantly related with sweet potato yield and disease index at harvest time (P < 0.05). Therefore, crop rotation significantly increased the diversity of nematode communities in sweet potato rhizosphere soils and sweet potato yield. And DGGE was proved to be a useful tool to detect soil nematode community. For the economic and ecological effects, bean-rye→sweet potato was the best rotation pattern for the study area.
HTML全文
图1不同轮作方式下甘薯根际土壤线虫ITS区扩增片段的DGGE图谱(泳道编号见表 1, B1-B15示条带)
Figure1.DGGE profiles of ITS gene fragments obtained from nematodes communities gDNA in sweet potatoes rhizosphere soil of different rotation patterns (Numbers 1-16 are shown in Table 1, B1-B15 are codes of the bands)
下载: 全尺寸图片幻灯片
图2不同轮作方式下甘薯根际线虫群落的聚类图谱(编号见表 1)
Figure2.Cluster analysis of soil nematode community in sweet potatoes rhizosphere soil of different rotation patterns (Numbers 1-16 are shown in Table 1)
下载: 全尺寸图片幻灯片
图3不同轮作方式下甘薯根际线虫群落的主成分分析(编号见表 1)
Figure3.Principal component analysis of soil nematode community in sweet potatoes rhizosphere soil of different planting patterns (Numbers 1-16 are shown in Table 1)
下载: 全尺寸图片幻灯片
图4不同轮作方式甘薯根际土壤优势线虫群落的比例分布图(编号见表 1)
Figure4.Diagram of dominant nematode genus in sweet potatoes rhizosphere soil of different rotation patterns (Numbers 1-16 are shown in Table 1)
下载: 全尺寸图片幻灯片
图5不同轮作方式的甘薯茎线虫数量变化
不同字母表示不同处理不同时期差异显著。
Figure5.Quantities of Ditylenchus destructor of sweet potato soil in different rotation patterns
Different lowercase letters show significant differences among different treatments at different periods at 0.05 level.
下载: 全尺寸图片幻灯片表1不同轮作方式下甘薯根际土壤线虫群落多样性和丰富度指数
Table1.Shannon diversity and richness indexes of soil nematode community in sweet potatoes rhizosphere soil of different rotation patterns
| 泳道 Lane | 处理Treatment | 采样时间 Sampling time | 多样性指数(H) hannon Winner index | 丰富度(S) Richness | |
| 轮作方式Rotation pattern | 代码Code | ||||
| 1 | 休闲→甘薯 Fallow→sweet potato | A1 | 5月May | 1.54e | 5.12e |
| 2 | 7月July | 1.99b | 9.33bc | ||
| 3 | 9月September | 1.75d | 7.00d | ||
| 4 | 玉米—冬闲→甘薯 Maize—fallow→sweet potato | A2 | 5月May | 1.91bc | 10.52ab |
| 5 | 7月July | 1.92bc | 10.93a | ||
| 6 | 9月September | 1.99b | 11.10a | ||
| 7 | 玉米—黑麦→甘薯 Maize—rye→sweet potato | A3 | 5月May | 1.75d | 7.24d |
| 8 | 7月July | 1.79cd | 7.77d | ||
| 9 | 9月September | 1.73d | 7.76d | ||
| 10 | 大豆—冬闲→甘薯 Soybean—fallow→ sweet potato | A4 | 5月May | 1.89c | 9.38bc |
| 11 | 7月July | 2.06ab | 9.44bc | ||
| 12 | 9月September | 1.95bc | 9.11bc | ||
| 13 | 大豆—黑麦→甘薯 Soybean—rye→sweet potato | A5 | 5月May | 2.13a | 10.00b |
| 14 | 7月July | 2.08ab | 10.00b | ||
| 15 | 9月September | 2.09ab | 9.01c | ||
| 16 | 甘薯连作 Sweet potato continuous cropping | CK | 9月September | 1.52e | 5.22e |
| 同列不同小写字母表示在0.05水平差异显著(n=3)。Different lowercase letters in the same column mean significant differences at 0.05 level (n = 3). | |||||
下载: 导出CSV表2不同轮作方式下甘薯根际线虫群落DGGE图谱优势特征条带的ITS序列(条带标号见图 1)
Table2.ITS sequences of dominant and special bands from DGGE gel of soil nematode community in sweet potatoes rhizosphere soil of different rotation patterns (band codes are shown in Fig. 1)
| 条带B and | 属名 Genus | 种名 Species | Genbank登录号 Access No. | 相似度Similarity (%) |
| B1 | 矛线虫属Dorylaimus | D. stagnalis | AY592995 | 98 |
| B2 | 茎线虫属Ditylenchus | D. destructor | KJ567140 | 91 |
| B3 | 滑刃线虫属Aphelenchoides | A. paradalianensis | GU337993 | 100 |
| B4 | 头叶线虫属Cephalobus | Cephalobus sp. | EU040137 | 94 |
| B5 | 短体线虫属Pratylenchus | P. hippeastri | EU040126 | 100 |
| B6 | 小环线虫属Criconemella | C. propinquus | AY284677 | 97 |
| B7 | 刺线虫属Belonolaimus | B. vexilliger | KC509905 | 96 |
| B8 | 矛线虫属Dorylaimus | D. stagnalis | AY592994 | 100 |
| B9 | 真滑刃线虫属Aphelenchus | Aphelenchus sp. | AB368918 | 91 |
| B10 | 双胃线虫属Diplogaster | Diplogaster sp. | FJ516756 | 97 |
| B11 | 矛线虫属Dorylaimus | D. stagnalis | AY284777 | 100 |
| B12 | 头叶线虫属Cephalobus | Cephalobus sp. | AB631026 | 99 |
| B13 | 环节动物门Annelida | Eisenia fetida | X79872 | 100 |
| B14 | 环节动物门Annelida | Cambarincola floridanus | JQ821667 | 96 |
| B15 | 环节动物门Annelida | Uncultured Acanthobdellida spp. | EF024636 | 99 |
下载: 导出CSV表3不同轮作方式下甘薯的产量及病情指数
Table3.Yields and disease indexes of sweet potatoes in different rotation patterns
| 处理Treatment | 甘薯产量 Yield (kg·hm-2) | 病情指数 Disease index | |
| 轮作方式Rotation pattern | 代码Code | ||
| 休闲→甘薯 Fallow→sweet potato | A1 | 11 372.40±32.32ab | 33.24±1.04b |
| 玉米—冬闲→甘薯 Maize—fallow→sweet potato | A2 | 11 672.55±23.64ab | 30.79±0.97b |
| 玉米—黑麦→甘薯 Maize—rye→sweet potato | A3 | 12 472.95±25.50a | 34.38±0.99b |
| 大豆—冬闲→甘薯 Soybean—fallow→ sweet potato | A4 | 11 629.20±18.63ab | 32.49±1.35b |
| 大豆—黑麦→甘薯 Soybean—rye→sweet potato | A5 | 12 310.95±19.66a | 31.63±1.01b |
| 甘薯连作 Sweet potato continuous cropping | CK | 8 004.00±29.33c | 44.49±1.52a |
| 同列不同小写字母表示在0.05水平差异显著(n=3)。Different small letters in the same column mean significant differences at 0.05 level (n = 3). | |||
下载: 导出CSV表4不同轮作方式的甘薯产量及病情指数与茎线虫数量的相关关系
Table4.Correlation between yield, disease index of sweet potatoes and quantities of Ditylenchus destructor in different rotation patterns
| 处理Treatment | 时期 Period | 甘薯产量 Yield of sweet potatoes | 病情指数 Disease index | |
| 轮作方式 Rotation pattern | 代码Code | |||
| 休闲→甘薯 Fallow→sweet potato | A1 | 5月May | -0.465 2 | -0.545 8 |
| 7月July | 0.465 2 | 0.545 8 | ||
| 9月September | -0.995 2 | 0.995 8* | ||
| 玉米—冬闲→甘薯 Maize—fallow→ sweet potato | A2 | 5月May | 0.585 5 | 0.476 9 |
| 7月July | 0.795 3 | 0.597 6 | ||
| 9月September | -0.101 7 | 0.026 5 | ||
| 玉米—黑麦→甘薯 Maize—rye→ sweet potato | A3 | 5月May | -0.438 8 | -0.439 8 |
| 7月July | 0.069 2 | 0.068 2 | ||
| 9月September | -0.539 0 | -0.539 9 | ||
| 大豆—冬闲→甘薯 Soybean—fallow→ sweet potato | A4 | 5月May | 0.438 1 | -0.434 1 |
| 7月July | -0.629 1 | 0.625 7 | ||
| 9月September | -0.670 2 | 0.666 9 | ||
| 大豆—黑麦→甘薯 Soybean—rye→ sweet potato | A5 | 5月May | 0.434 4 | 0.502 9 |
| 7月July | 0.434 4 | 0.502 9 | ||
| 9月September | -0.996 8** | -0.657 2 | ||
| 甘薯连作 Sweet potato continuous cropping | CK | 5月May | -0.487 4 | 0.577 4 |
| 7月July | 0.484 2 | -0.574 5 | ||
| 9月September | -0.997 2** | 0.997 4** | ||
| *: 0.05 < P < 0.1; **: 0.01 < P < 0.05; ***: 0.001 < P < 0.01. | ||||
下载: 导出CSV参考文献
| [1] | BONGERS T, BONGERS M. Functional diversity of nematodes[J]. Applied Soil Ecology, 1998, 10(3):239-251 doi: 10.1016/S0929-1393(98)00123-1 |
| [2] | NEHER D A. Nematode communities in organically and conventionally managed agricultural soils[J]. Journal of Nematology, 1999, 31(2):142-154 http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_2620368 |
| [3] | YEATES G W. Nematodes as soil indicators:Functional and biodiversity aspects[J]. Biology & Fertility of Soils, 2003, 37(4):199-210 doi: 10.1007/s00374-003-0586-5 |
| [4] | BONGERS T, FERRIS H. Nematode community structure as a bioindicator in environmental monitoring[J]. Trends in Ecology & Evolution, 1999, 14(6):224-228 http://www.sciencedirect.com/science/article/pii/S0169534798015833 |
| [5] | NEHER D A. Role of nematodes in soil health and their use as indicators[J]. Journal of Nematology, 2001, 33(4):161-168 http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_2620512 |
| [6] | 卢肖平.我国甘薯与马铃薯的生产发展、科研成就及其展望[J].中国农学通报, 1996, 12(1):7-9 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199600703080 LU X P. Production development, scientific research achievements and prospects of sweet potato and potato in our country[J]. Chinese Agricultural Science Bulletin, 1996, 12(1):7-9 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=QK199600703080 |
| [7] | 谢逸萍, 孙厚俊, 邢继英.中国各大薯区甘薯病虫害分布及危害程度研究[J].江西农业学报, 2009, 21(8):121-122 doi: 10.3969/j.issn.1001-8581.2009.08.042 XIE Y P, SUN H J, XING J Y. Study on potato distribution and the damage of the sweet potato plant diseases in China[J]. Acta Agriculturae Jiangxi, 2009, 21(8):121-122 doi: 10.3969/j.issn.1001-8581.2009.08.042 |
| [8] | 周忠, 马代夫.甘薯茎线虫病的研究现状和展望[J].杂粮作物, 2003, 23(5):288-290 doi: 10.3969/j.issn.2095-0896.2003.05.014 ZHOU Z, MA D F. The research status of sweet potato stem nematode and prospected[J]. Rain Fed Crops, 2003, 23(5):288-290 doi: 10.3969/j.issn.2095-0896.2003.05.014 |
| [9] | 高德良, 于伟丽, 苗建强, 等.几种替代杀线剂对甘薯茎线虫的毒力与活性[J].应用生态学报, 2011, 22(11):3026-3032 http://d.old.wanfangdata.com.cn/Periodical/yystxb201111033 GAO D L, YU W L, MIAO J Q, et al. Toxicity and bioactivity of several alternative nematocides against Ditylenchus destructor[J]. Chinese Journal of Applied Ecology, 2011, 22(11):3026-3032 http://d.old.wanfangdata.com.cn/Periodical/yystxb201111033 |
| [10] | 李旭.不同药剂防治甘薯茎线虫病试验初报[J].安徽农学通报, 2016, 22(7):67, 78 http://d.old.wanfangdata.com.cn/Periodical/ahnxtb201607034 LI X. Different drugs for controlling sweet potato stem nematode disease[J]. Anhui Agricultural Science Bulletin, 2016, 22(7):67, 78 http://d.old.wanfangdata.com.cn/Periodical/ahnxtb201607034 |
| [11] | 孔德洋, 朱忠林, 石利利, 等.中国北方甘薯地农药使用对地下水水质的影响[J].农业环境科学学报, 2004, 23(5):1017-1020 doi: 10.3321/j.issn:1672-2043.2004.05.040 KONG D Y, ZHU Z L, SHI L L, et al. Effect of pesticides on groundwater under sweet-potato-based cropping systems in northern China[J]. Journal of Agro-Environment Science, 2004, 13(5):1017-1020 doi: 10.3321/j.issn:1672-2043.2004.05.040 |
| [12] | 杨文兰, 刘贺昌, 张卫青, 等.克线丹颗粒剂对甘薯茎线虫病的防治效果[J].河北科技师范学院学报, 2006, 20(1):25-28 doi: 10.3969/j.issn.1672-7983.2006.01.007 YANG W L, LIU H C, ZHANG W Q, et al. Effect of rugby granular on sweet potato stem nematode[J]. Journal of Hebei Normal University of Science & Technology, 2006, 20(1):25-28 doi: 10.3969/j.issn.1672-7983.2006.01.007 |
| [13] | 朱育菁, 肖荣凤, 林抗美, 等.维线克对甘薯茎线虫的田间防治效果[J].福建农业学报, 2005, 20(3):139-141 doi: 10.3969/j.issn.1008-0384.2005.03.003 ZHU Y J, XIAO R F, LIN K M, et al. Control efficiency of Weixianke to sweet potato stem nematode[J]. Fujian Journal of Agricultural Sciences, 2005, 20(3):139-141 doi: 10.3969/j.issn.1008-0384.2005.03.003 |
| [14] | 李秀花, 高波, 马娟, 等.休闲与轮作对燕麦孢囊线虫种群动态的影响[J].麦类作物学报, 2013, 33(5):1048-1053 http://d.old.wanfangdata.com.cn/Periodical/mlzwxb201305035 LI X H, GAO B, MA J, et al. Effect of fallow and rotation on the population dynamics of Heterodera avenae[J]. Journal of Triticeae Crops, 2013, 33(5):1048-1053 http://d.old.wanfangdata.com.cn/Periodical/mlzwxb201305035 |
| [15] | 靳学慧, 辛惠普, 郑雯, 等.长期轮作和连作对土壤中大豆胞囊线虫数量的影响[J].中国油料作物学报, 2006, 28(2):189-193 doi: 10.3321/j.issn:1007-9084.2006.02.017 JIN X H, XIN H P, ZHENG W, et al. The influence of soil on the long-term rotation and continuous cultivation on soybean cyst nematode[J]. Chinese Journal of Oil Crop Sciences, 2006, 28(2):189-193 doi: 10.3321/j.issn:1007-9084.2006.02.017 |
| [16] | 金凤柱, 海棠, 武宝悦, 等.几种不同农艺措施对土壤甘薯茎线虫种群动态的影响[J].中国生态农业学报, 2008, 16(4):921-924 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?file_no=2008423&flag=1 JIN F Z, HAI T, WU B Y, et al. Effect of agronomic measures on population dynamics of sweet potato stem nematode (Ditylenchus destructor)[J]. Chinese Journal of Eco-Agriculture, 2008, 16(4):921-924 http://www.ecoagri.ac.cn/zgstny/ch/reader/view_abstract.aspx?file_no=2008423&flag=1 |
| [17] | 陈立杰, 朱艳, 刘彬, 等.连作和轮作对大豆胞囊线虫群体数量及土壤线虫群落结构的影响[J].植物保护学报, 2007, 34(4):347-352 doi: 10.3321/j.issn:0577-7518.2007.04.003 CHEN L J, ZHU Y, LIU B, et al. Influence of continuous cropping and rotation on soybean cyst nematode and soil nematode community structure[J]. Acta Phytophylacica Sinica, 2007, 34(4):347-352 doi: 10.3321/j.issn:0577-7518.2007.04.003 |
| [18] | 刘方明, 孟维韧.土壤线虫作为生态系统恢复指示生物的研究进展[J].安徽农业科学, 2008, 36(7):2913-2915 doi: 10.3969/j.issn.0517-6611.2008.07.128 LIU F M, MENG W R. Study advance of soil nematodes as bioindicator of ecosystem restoration[J]. Journal of Anhui Agricultural Sciences, 2008, 36(7):2913-2915 doi: 10.3969/j.issn.0517-6611.2008.07.128 |
| [19] | 宋敏, 刘银占, 井水水.土壤线虫对气候变化的响应研究进展[J].生态学报, 2015, 35(20):6857-6867 http://d.old.wanfangdata.com.cn/Periodical/stxb201520032 SONG M, LIU Y Z, JING S S. Response of soil nematodes to climate change:a review[J]. Acta Ecologica Sinica, 2015, 35(20):6857-6867 http://d.old.wanfangdata.com.cn/Periodical/stxb201520032 |
| [20] | GRABAU Z J, CHEN S Y. Influence of long-term corn-soybean crop sequences on soil ecology as indicated by the nematode community[J]. Applied Soil Ecology, 2016, 100:172-185 doi: 10.1016/j.apsoil.2015.12.016 |
| [21] | 王明伟, 刘雨迪, 陈小云, 等.旱地红壤线虫群落对不同耕作年限的响应及指示意义[J].土壤学报, 2016, 53(2):510-522 http://d.old.wanfangdata.com.cn/Periodical/trxb201602023 WANG M W, LIU Y D, CHEN X Y, et al. Response of soil nematode community to cultivation in upland red soil relative to cultivation history and its signifi cance as indicator[J]. Acta Pedologica Sinica, 2016, 53(2):510-522 http://d.old.wanfangdata.com.cn/Periodical/trxb201602023 |
| [22] | 张伟东, 吕莹, 肖莹, 等.线虫群落对抚顺煤矸石山周边土壤可溶性盐污染的响应[J].生态学报, 2012, 32(8):2439-2446 http://d.old.wanfangdata.com.cn/Periodical/stxb201208015 ZHANG W D, LYU Y, XIAO Y, et al. Responses of soil nematode communities to soluble salt contamination around Gangue hill in Fushun[J]. Acta Ecologica Sinica, 2012, 32(8):2439-2446 http://d.old.wanfangdata.com.cn/Periodical/stxb201208015 |
| [23] | 王进闯, 王敬国.大豆连作土壤线虫群落结构的影响[J].植物营养与肥料学报, 2015, 21(4):1022-1031 http://d.old.wanfangdata.com.cn/Periodical/zwyyyflxb201504022 WANG J C, WANG J G. Effects of continuous soybean monoculture on soil nematode community[J]. Journal of Plant Nutrition and Fertilizer, 2015, 21(4):1022-1031 http://d.old.wanfangdata.com.cn/Periodical/zwyyyflxb201504022 |
| [24] | QIAO Y J, LI Z Z, WANG X, et al. Effect of legume-cereal mixtures on the diversity of bacterial communities in the rhizosphere[J]. Plant, Soil & Environment, 2012, 58(4):174-180 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=9f9d4a8341200b4cebdb07c36e0fa190 |
| [25] | MATSUSHITA Y, YAMAMURA K, MORIMOTO S, et al. Analysis of variations in band positions for normalization in across-gel denaturing gradient gel electrophoresis[J]. Journal of Microbiological Methods, 2015, 112:11-20 doi: 10.1016/j.mimet.2015.02.011 |
| [26] | WAITE I S, O'DONNELL A G, HARRISON A, et al. Design and evaluation of nematode 18S rDNA primers for PCR and denaturing gradient gel electrophoresis (DGGE) of soil community DNA[J]. Soil Biology and Biochemistry, 2003, 35(9):1165-1173 doi: 10.1016/S0038-0717(03)00177-9 |
| [27] | FOUCHER A, WILSON M. Development of a polymerase chain reaction-based denaturing gradient gel electrophoresis technique to study nematode species biodiversity using the 18S rDNA gene[J]. Molecular Ecology Notes, 2002, 2(1):45-48 doi: 10.1046/j.1471-8286.2002.00141.x |
| [28] | WANG S B, LI Q, LIANG W J, et al. PCR-DGGE analysis of nematode diversity in Cu-contaminated soil[J]. Pedosphere, 2008, 18(5):621-627 doi: 10.1016/S1002-0160(08)60056-9 |
| [29] | DJIGAL D, BRAUMAN A, DIOP T A, et al. Influence of bacterial-feeding nematodes (Cephalobidae) on soil microbial communities during maize growth[J]. Soil Biology and Biochemistry, 2004, 36(2):323-331 doi: 10.1016/j.soilbio.2003.10.007 |
| [30] | ZHOU J H, SUN X W, JIAO J G, et al. Dynamic changes of bacterial community under the influence of bacterial-feeding nematodes grazing in prometryne contaminated soil[J]. Applied Soil Ecology, 2013, 64:70-76 doi: 10.1016/j.apsoil.2012.11.005 |
| [31] | 毛小芳, 李辉信, 陈小云, 等.土壤线虫三种分离方法效率比较[J].生态学杂志, 2004, 23(3):149-151 http://d.old.wanfangdata.com.cn/Periodical/stxzz200403033 MAO X F, LI H X, CHEN X Y, et al. Extraction efficiency of soil nematodes by different methods[J]. Chinese Journal of Ecology, 2004, 23(3):149-151 http://d.old.wanfangdata.com.cn/Periodical/stxzz200403033 |
| [32] | FERRIS V R, FERRIS J M, FAGHIHI J. Variation in spacer ribosomal DNA in some cyst-forming species of plant parasitic nematodes[J]. Fundamental & Applied Nematology, 1993, 16(2):177-184 |
| [33] | 王明祖.中国植物线虫研究[M].武汉:湖北科学技术出版社, 1998 WANG M Z. Chinese Plant Nematode Research[M]. Wuhan:Hubei Science and Technology Press, 1998 |
| [34] | SHANNON C E, WEAVER W. The Mathematical Theory of Communication[M]. Urbana:The University of Illinois Press, 1949 |
| [35] | ZHONG S, ZENG H C, JIN Z Q. Responses of soil nematode abundance and diversity to long-term crop rotations in tropical China[J]. Pedosphere, 2015, 25(6):844-852 doi: 10.1016/S1002-0160(15)30065-5 |
| [36] | 洪毅, 张伟东, 王雪峰, 等.庄河口湿地不同土地利用方式下土壤线虫群落特征[J].生态环境学报, 2014, 23(10):1616-1621 doi: 10.3969/j.issn.1674-5906.2014.10.008 HONG Y, ZHANG W D, WANG X F, et al. The characteristic of soil nematodes communities under different vegetation types in Zhuanghe estuary wetland[J]. Ecology and Environmental Sciences, 2014, 23(10):1616-1621 doi: 10.3969/j.issn.1674-5906.2014.10.008 |
| [37] | 王会芳, 肖彤斌, 芮凯, 等.不同技术措施对蔬菜根结线虫种群的影响[J].植物保护, 2010, 36(2):161-164 doi: 10.3969/j.issn.0529-1542.2010.02.040 WANG H F, XIAO T B, RUI K, et al. Effects of different technical measures on populations of the root-knot nematodes on vegetables[J]. Plant Protection, 2010, 36(2):161-164 doi: 10.3969/j.issn.0529-1542.2010.02.040 |
| [38] | 高学彪, 周慧娟, 冯志新.几种农业措施对水稻潜根线虫病的防治作用及机理的研究[J].华中农业大学学报, 1998, 17(4):331-334 doi: 10.3321/j.issn:1000-2421.1998.04.006 GAO X B, ZHOU H J, FENG Z X. Efficacy and mechanism of cultivation measures on Hirschmanniella oryzae[J]. Journal of Huazhong Agricultural University, 1998, 17(4):331-334 doi: 10.3321/j.issn:1000-2421.1998.04.006 |
| [39] | 海棠, 彭德良, 曾昭海, 等.轮作对甘薯地线虫群落组成及多样性的影响[J].中国农学通报, 2006, 22(11):295-298 doi: 10.3969/j.issn.1000-6850.2006.11.070 HAI T, PENG D L, ZENG Z H, et al. Effect of rotate on nematode community composition and diversity in sweet potato field[J]. Chinese Agricultural Science Bulletin, 2006, 22(11):295-298 doi: 10.3969/j.issn.1000-6850.2006.11.070 |
| [40] | 海棠, 彭德良, 曾昭海, 等.耕作制度对甘薯地土壤线虫群落结构的影响[J].中国农业科学, 2008, 41(6):1851-1857 doi: 10.3864/j.issn.0578-1752.2008.06.037 HAI T, PENG D L, ZENG Z H, et al. Effect of cropping systems on nematode community structure in sweet potato field[J]. Scientia Agricultura Sinica, 2008, 41(6):1851-1857 doi: 10.3864/j.issn.0578-1752.2008.06.037 |
| [41] | CROW W T. Amaryllis lesion nematode, Pratylenchus hippeastri[Z]. EENY-546. University of Florida IFAS, 2012 |
| [42] | DAVIS R F, KOENNING S R, KEMERAIT R C, et al. Rotylenchulus reniformis management in cotton with crop rotation[J]. Journal of Nematology, 2003, 35(1):58-64 http://d.old.wanfangdata.com.cn/OAPaper/oai_pubmedcentral.nih.gov_2620609 |
| [43] | KORTHALS G W, NIJBOER H, MOLENDIJK L P G. Meloidogyne chitwoodi:Host plant suitability of field crops and cover crops[J]. PAV Bulletin Akkerbouw, 2000:1-3 http://www.cabdirect.org/abstracts/20001701062.html |
| [44] | 刘淑霞, 潘冬梅, 魏国江, 等.轮作防治大豆胞囊线虫病的研究现状[J].黑龙江科学, 2011, 2(1):35-36 http://d.old.wanfangdata.com.cn/Periodical/hljkx201101010 LIU S X, PAN D M, WEI G J, et al. Current research on controlling soybean cyst nematode by crop rotation[J]. Heilongjiang Science, 2011, 2(1):35-36 http://d.old.wanfangdata.com.cn/Periodical/hljkx201101010 |
| [45] | 孙玉秋, 许艳丽, 李春杰, 等.作物轮作系统对大豆胞囊线虫二龄幼虫寄生真菌的影响[J].大豆科学, 2011, 30(2):277-280 http://d.old.wanfangdata.com.cn/Periodical/ddkx201102022 SUN Y Q, XU Y L, LI C J, et al. Effects of crop rotation system on the parasitic fungi associated with soybean cyst nematode second-stage juvenile[J]. Soybean Science, 2011, 30(2):277-280 http://d.old.wanfangdata.com.cn/Periodical/ddkx201102022 |
| [46] | 乔月静, 王雪娇, 武宝悦, 等.不同种植模式对甘薯产量及甘薯根际茎线虫数量的影响[J].农业现代化研究, 2014, 35(6):800-803 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=663027820 QIAO Y J, WANG X J, WU B Y. Effect of different planting patterns on yield of sweet potato and the number of nematodes in rhizosphere[J]. Research of Agricultural Modernization, 2014, 35(6):800-803 http://www.wanfangdata.com.cn/details/detail.do?_type=perio&id=663027820 |
