Effect of Wilt-Resistant and Wilt-Susceptible Cotton on Soil Bacterial Community Structure at Flowering and Boll Stage
ZHAO WeiSong, GUO QingGang, LI SheZeng, WANG PeiPei, LU XiuYun, SU ZhenHe, ZHANG XiaoYun, MA Ping,Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China, Ministry of Agriculture and Rural Affairs, Baoding 071000, Hebei
Abstract 【Objective】The objective of this study is to research soil bacterial community structure of cotton verticillium wilt resistant and susceptible varieties at flowering and boll stage, understand the relationship between soil bacterial community structure and soil physicochemical properties, and to provide a theoretical basis for monitoring of cotton verticillium wilt and green ecological control. 【Method】The susceptible (EJ) and resistant (J863) varieties were used in field plot experiments. The ITS gene copy number of Verticillium dahliae and soil bacterial community structure at different flowering and boll stages (flowering, late flowering, and boll-forming stages) were studied by real-time fluorescence quantitative PCR (real-time PCR) and high throughput sequencing (Illumina MiSeq), respectively. The redundancy analysis (RDA) was used to determine the correlation between bacterial community structure and soil physicochemical properties. 【Result】There were different degrees of relevance between the occurrence of verticillium wilt and the ITS gene copy number of V. dahliae in soil. The incidence and disease index of susceptible variety EJ were positively correlated with the ITS gene copy number of V. dahliae, while those of resistant variety J863 were not significantly correlated with the ITS gene copy number of V. dahliae. The ITS gene copy number of V. dahliae in soil of resistant variety J863 was lower than that of susceptible variety EJ at late flowering and boll-forming stages. The high throughput sequencing analysis showed that the bacterial richness indices (Chao1 and ACE) of resistant variety J863 were higher than those of susceptible variety EJ at flowering and boll-forming stages. The principal component analysis showed that there were differences in soil bacterial community structure between resistant and susceptible varieties and at different flowering and boll stages. At phylum level, the average relative abundance of some dominant bacteria in susceptible variety EJ was lower than that of resistant variety J863, such as Actinobacteria, Gemmatimonadetes, Chloroflexi, Bacteroidetes, Nitrospirae, Patescibacteria, and Armatimonadetes, which was decreased by 16.38%, 4.05%, 2.25%, 6.58%, 7.10%, 20.60%, and 35.78%, respectively. At genus level, the average relative abundance of some dominant bacteria of susceptible variety EJ was lower than that of resistant variety J863, including Sphingomonas, Gemmatimonas, Bryobacter, Iamia, Pseudarthrobacter, Blastococcus, Rubrobacter, Nocardioides, Pontibacter, Streptomyces, Gemmatirosa, Micromonospora, and Solirubrobacter, which was decreased by 5.09%, 19.41%, 13.79%, 2.36%, 10.78%, 34.47%, 46.76%, 61.84%, 52.75%, 48.61%, 74.79%, 9.13%, and 26.42%, respectively. The redundancy analysis (RDA) showed that the bacterial community structure was affected by nitrate nitrogen (NO3--N), available phosphorus (AP), ammonium nitrogen (NH4+-N), inorganic phosphorus (IP), pH and organic matter (OM). 【Conclusion】The correlation between the ITS gene copy number of V. dahliae in soil and the occurrence of cotton verticillium wilt of susceptible and resistant varieties is different, and the incidence of verticillium wilt in susceptible variety is positively correlated with the ITS gene copy number of V. dahliae in soil. The bacterial community structure of resistant variety is better than susceptible variety at flowering, late flowering, and boll-forming stages. There are some differences in the dominant bacterial community at different growth stages. The diversity, relative abundance and composition of bacteria in soil are affected by organic matter, pH, nitrogen type and available phosphorus. Meanwhile, the growth stage of cotton had a significant influence on the bacterial community structure in soil. Keywords:cotton verticillium wilt;Verticillium dahliae;disease resistance;flowering and boll stage;bacterial community structure;Illumina MiSeq;soil physicochemical properties
PDF (1808KB)元数据多维度评价相关文章导出EndNote|Ris|Bibtex收藏本文 本文引用格式 赵卫松, 郭庆港, 李社增, 王培培, 鹿秀云, 苏振贺, 张晓云, 马平. 花铃期棉花黄萎病抗病与感病品种对 土壤细菌群落结构的影响[J]. 中国农业科学, 2020, 53(5): 942-954 doi:10.3864/j.issn.0578-1752.2020.05.007 ZHAO WeiSong, GUO QingGang, LI SheZeng, WANG PeiPei, LU XiuYun, SU ZhenHe, ZHANG XiaoYun, MA Ping. Effect of Wilt-Resistant and Wilt-Susceptible Cotton on Soil Bacterial Community Structure at Flowering and Boll Stage[J]. Scientia Acricultura Sinica, 2020, 53(5): 942-954 doi:10.3864/j.issn.0578-1752.2020.05.007
Table 2 表2 表2棉花抗/感品种在花铃期不同阶段的细菌测序量和群落多样性 Table 2Bacterial sequencing and community diversity of cotton resistant/susceptible varieties at different flowering and boll stages
红色、绿色和黄色分别代表盛花期、开花后期和结铃期,星形代表感病品种鄂荆1号(EJ),圆形代表抗病品种冀863(J863),椭圆代表品种在不同时期的微生物群落组成Red, green and yellow represent flowering, late flowering and boll-forming stages, respectively. The star represents the susceptible variety EJ, and the round represents the resistant variety J863. The ellipse represents the microbial community composition of the variety at different stages门水平Phylum level:Proteobacteria变形菌门;Acidobacteria酸杆菌门;Actinobacteria放线菌门;Gemmatimonadetes芽单孢菌门;Chloroflexi绿弯菌门;Bacteroidetes拟杆菌门;Planctomycetes浮霉菌门;Nitrospirae硝化螺旋菌门;Verrucomicrobia疣微菌门;Armatimonadetes装甲菌门;Firmicutes 厚壁菌门。属水平Genus level:Sphingomonas鞘氨醇单胞菌属;Lysobacter溶杆菌属;Gemmatimonas芽单胞菌属;Nitrospira硝化螺菌属;Blastococcus芽球菌属;Rubrobacter红色杆菌属;Nocardioides类诺卡氏属;Streptomyces链霉菌属;Micromonospora微单孢菌属 Fig. 3Principal component analysis of soil bacterial community structure of cotton resistant/susceptible varieties at phylum (A) and genus (B) levels
红色、绿色和黄色分别代表盛花期、开花后期和结铃期,星形代表感病品种鄂荆1号(EJ),圆形代表抗病品种冀863(J863)Red, green and yellow represent flowering, late flowering and boll-forming stages, respectively. The star represents the susceptible variety EJ, and the round represents the resistant variety J863 Fig. 6RDA analysis of soil physicochemical properties and bacterial communities at genus level
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