\r王园园¹，刘晓非¹，邹雅露¹，张 海¹，郑淇方¹，赵励彦¹，吴蕴棠\r²\r
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AuthorsHTML:\r王园园¹，刘晓非¹，邹雅露¹，张 海¹，郑淇方¹，赵励彦¹，吴蕴棠\r²\r
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AuthorsListE:\rWang Yuanyuan¹，Liu Xiaofei¹，Zou Yalu¹，Zhang Hai¹，Zheng Qifang¹，Zhao Liyan¹，Wu Yuntang\r ²\r
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AuthorsHTMLE:\rWang Yuanyuan¹，Liu Xiaofei¹，Zou Yalu¹，Zhang Hai¹，Zheng Qifang¹，Zhao Liyan¹，Wu Yuntang\r ²\r
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Unit:\r\r1. 天津大学材料科学与工程学院，天津 300072；\r
\r\r2. 天津医科大学公共卫生学院，天津 300072\r
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Unit_EngLish:\r1. School of Materials Science and Engineering，Tianjin University，Tianjin 300072，China；
2. School of Public Health，Tianjin Medical University，Tianjin 300072，China\r
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Abstract_Chinese:\r\r针对壳寡糖胍\r(\rCOSG\r)\r对动物体内胰岛素抵抗治疗效果及相关机制尚不明确的问题，建立高脂高糖饮食联合链脲佐菌素诱导的\r2\r型糖尿病\r(\rT2DM\r)\r大鼠模型，并进行\rCOSG\r灌胃给药治疗\r8\r周，研究\rCOSG\r对\rT2DM\r大鼠体内胰岛素抵抗及相关信号通路的影响．结果显示，\rCOSG\r可明显降低\rT2DM\r大鼠血清中总胆固醇\r(\rTC\r)\r、甘油三酯\r(\rTG\r)\r和低密度脂蛋白\r(\rLDL\r)\r水平并提高血清中高密度脂蛋白\r(\rHDL\r)\r水平；此外，\rCOSG\r可下调\rT2DM\r大鼠的空腹血糖\r(\rFBG\r)\r和空腹胰岛素\r(\rFINS\r)\r含量，并减小\rT2DM\r大鼠胰岛素抵抗指数\r(\rHOMA\r-\rIR\r)\r．进一步研究相关蛋白发现，\rCOSG\r可抑制骨骼肌中\rp38 \r丝裂原活化蛋白激酶\r(\rp3\r8\rMAPK\r)\r的磷酸化以及提高胰岛素受体底物\r-\r1\r(\rIRS\r-\r1\r)\r酪氨酸位点的磷酸化，并提高蛋白激酶\rB\r(\rAKT\r)\r的磷酸化水平和促进葡萄糖转运蛋白\r4\r(\rGLUT4\r)\r转移至细胞膜，从而促进细胞对葡萄糖的摄取；另外，\rCOSG\r还可以降低磷酸烯醇式丙酮酸羧激酶\r(\rPEPCK\r)\r和肌糖原水平，进而抑制糖异生\r. 从而，COSG达到改善胰岛素抵抗的作用．总之，COSG可改善T2DM大鼠胰岛素抵抗，具有良好的应用前景.\r
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Abstract_English:\r\rThe effects of chitooligosaccharide guanidine (COSG) on insulin resistance and its related in vivo mechanisms still remain unclear. Therefore, in this study, a type 2 diabetic (T2DM) rat model was established using a high-fat and high-sugar diet combined with streptozotocin, followed by intragastric administration of COSG for 8 weeks, to investigate the effects of COSG on insulin resistance and the related signaling pathways in T2DM rats. Results demonstrated that COSG significantly reduced the levels of total cholesterol (TC), triglycerides (TGs), and low-density lipoprotein (LDL) and increased the levels of high-density lipoprotein (HDL) in the serum of T2DM rats. Furthermore, COSG downregulated the levels of fasting blood glucose (FBG) and fasting insulin (FINS) and decreased the homeostatic model assessment for insulin resistance (HOMA-IR) index in T2DM rats. Further analysis of related proteins showed that COSG inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), increased the tyrosine phosphorylation of insulin receptor substrate-1 (IRS-1) proteins, increased the phosphorylation of protein kinase B (Akt), and promoted the transfer of GLUT4 to the cell membrane, thereby promoting the uptake of glucose by cells. Results also showed that COSG can reduce the levels of phosphoenolpyruvate carboxykinase (PEPCK) and muscle glycogen, thereby inhibiting gluconeogenesis. Therefore, COSG has the effect of improving insulin resistance. In conclusion, COSG can improve insulin resistance in T2DM rats, a finding that has a good application prospect.\r\r
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Keyword_Chinese:壳寡糖胍；2 型糖尿病；胰岛素抵抗；葡萄糖转运蛋白4；胰岛素信号蛋白\r

Keywords_English:chitooligosaccharide guanidine；type 2 diabetes(T2DM)；insulin resistance；glucose transporter 4(GLUT4)；insulin signaling protein\r


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