王仲孚
所属部门： 食品科学与工程学院
职务/职称： 教授
荣誉： 陕西省中青年科技创新领军人才入选者，陕西省特支计划人选，博士生导师
电子邮件： wangzhf@nwu.edu.cn
个人简介 王仲孚，博士，教授，博士生导师。1995年本科毕业于西北农林科技大学，2001年博士毕业于华南理工大学生物科学与工程研究中心，2002－2004年在美国新罕布什尔大学（UNH）结构生物学研究中心（CSB）作博士后研究，2004年回国到西北大学生命科学学院工作，2018年至今在西北大学食品科学与工程学院工作，现为西北大学糖生物学与糖工程研究中心主任。主要从事多糖/寡糖的结构与功能研究，主要工作包括基于生物质谱技术的高灵敏度分析和鉴定寡糖（链）的新方法和新技术的建立及应用；多糖精细结构解析及活性研究；多糖的可控性降解及其活性寡糖片段的分离制备和序列结构分析等。在国内外学术期刊发表SCI论文86篇，申请国家发明专利12项，参编学术著作1部。主持国家"863"计划项目、国家自然科学基金、国家重点研发计划子课题等国家级科研项目8项，省部级科研项目5项，横向项目1项。2015年获首届张树政糖科学奖优秀青年奖，2016年入选陕西省中青年科技创新领军人才，2017年入选陕西省高层次人才特殊支持计划科技创新领军人才，2017年获陕西高等学校科学技术奖一等奖1项（1/11）。
研究成果 1. Yangni Deng, Qian Liu, Tiantian Dang, Guiping Gong, Xiaoyi Chen, Rui Tang, Jing Sun, Shuang Song Linjuan Huang*, Zhongfu Wang *.Preparation, Structural Characterization and Bioactivity of 4-O-Methylglucuronoxylan from Artemisia sphaerocephala Krasch. Carbohydrate Polymers (2019), 222: 115009.
2. Tang Rui, Chen Xiaoyi, Dang Tiantian, Deng Yangni, Zou Zihua, Liu Qian, Gong Guiping, Song Shuang, Ma Fangli, Huang Linjuan*, Wang Zhongfu*.Lycium barbarum Polysaccharides Extent the Mean Lifespan of Drosophila melanogaster. Food & Function (2019), 10: 4231–4241.
3.Zhang Ying, Wang Bo, JinWanjun, Wen Yanan, Nan Lijing, Yang Mingming, Liu Rendan, Zhu Yuyang, Wang Chengjian,Huang Linjuan, Song Xuezheng*, Wang Zhongfu*.Sensitive and robust MALDI-TOF-MS glycomics analysis enabled by Girard'sreagent T on-target derivatization (GTOD) of reducing glycans. AnalyticaChimica Acta (2019), 1048: 105-114.
4.Chen Xiaoyi, Tang Rui, LiuTingting, Dai Wei, Liu Qian, Gong Guiping, Song Shuang, Hu Minghua, Huang Linjuan*, Wang Zhongfu*. Physicochemicalproperties, antioxidant activity and immunological effects in vitro ofpolysaccharides from Schisandra sphenanthera and Schisandra chinensis. InternationalJournal of Biological Macromolecules (2019), 131: 744–751.
5.Lu Yu, Li Cheng, Wei Ming,Jia Yue, Song Jingjing, Zhang Ying, Wang Chengjian, Huang Linjuan*, Wang Zhongfu*. Release, Separation, andRecovery of Monomeric Reducing N?Glycanswith Pronase E Combined with 9?ChloromethylChloroformate and Glycosylasparaginase. Biochemistry (2019), 58: 1120?1130.
6.Nan Lijing, Li Jiao, JinWanjun, Wei Ming, Tang Mengjun, Wang Chengjian, Gong Guiping, Huang Linjuan, ZhangYing* and Wang Zhongfu*.Comprehensive quali-quantitative profiling of neutral and sialylated O-glycomeby mass spectrometry based on oligosaccharide metabolic engineering andisotopic labeling. RSC Advances (2019), 9: 15694–15702.
7.Li Cheng, Wang Chengjian,Jin Wanjun, Han Jianli, Yang Meifang, Gao Xi, Huang Linjuan, Wang Zhongfu*. Mass SpectrometricAnalysis of N-Glycans of Glycoprotein Separated by SDS-PAGE Gel from GinkgoSeed. Chemical Journal of Chinese Universities (2019), 40(1): 69-75.
8.Lu Yu, Wang Chengjian,Liu Rendan, Jin Wanjun, Wen Yanan, Huang Linjuan*, Wang Zhongfu*.3-Amino-1-phenyl-2-pyrazoline-5-ketone as a heterobifunctional chromogenicreagent to derivatize reducing glycans for subsequent online LC/MS analysis. AnalyticalBiochemistry (2018), 549: 1-11.
9.Wang Chengjian, Yang Meifang,Gao Xi, Li Cheng, Zou Zihua, Han Jianli, Linjuan Huang* Wang Zhongfu*. The ammonia-catalyzed release of glycoproteinN-glycans, Glycoconjugate Journal (2018), 35: 411–420.
10.Wang Chengjian, QiangShan, Jin Wanjun, Song Xuezheng, Zhang Ying, Huang Linjuan, Wang Zhongfu*. Reductive chemicalrelease of N-glycans as 1-amino-alditols and subsequent9-fluorenylmethyloxycarbonyl labeling for MS and LC/MS analysis. Journalof Proteomics (2018), 187: 47–58.
11.Wang Chengjian, Lu Yu,Han Jianli, Jin Wanjun, Li Lingmei, Zhang Ying, Song Xuezheng, Huang Linjuan, Wang Zhongfu*. Simultaneous Release andLabeling of O- and N?GlycansAllowing for Rapid Glycomic Analysis by Online LC-UV-ESI-MS/MS. Journalof Proteome Research (2018), 17: 2345?2357.
12. GongGuiping, Dang Tiantian, Deng Yangni, Han Jianli, Zou Zihua, Jing Sun, ZhangYing, Liu Qian, Huang Linjuan, WangZhongfu*. Physicochemical properties and biological activities ofpolysaccharides from Lycium barbarum prepared by fractional precipitation. InternationalJournal of Biological Macromolecules (2018), 109: 611-618.
13. GongGuiping, Zhao Jixiang, Wang Chengjian, Wei Ming, Dang Tiantian, Deng Yangni,Sun Jing, Song Shuang, Huang Linjuan*, WangZhongfu*. Structural characterization and antioxidant activities of thedegradation products from Porphyra haitanensis polysaccharidesProcess Biochemistry (2018), 74: 185–193.
14. LiLingmei, Wang Changjian, Han Jianli, Liu Rendan, Wei Ming, Jin Wangjun, HuangLinjuan, Wang Zhongfu*. Massspectrometric analysis of N-glycosylation sites and N-glycans of β-conglycinin.ChemicalJournal of Chinese Universities (2018), 39(3): 427-434.
15. YangMingming, Nan Lijing, Jin Wanjun, Wang Chengjian, Huang Linjuan, Zhang Ying*, Wang Zhongfu*. Quali-quantitativeProfiling of Mucin-type O-Glycans in Normal and Tumor Cell Lines Based onOligosaccharide Metabolic Engineering Combined with Mass Spectrometry.Chemical Journal of Chinese Universities (2018), 39(9):1904-1912.
16. SunGuosheng, Wu Yue, Liu Anqi, Qiu Saifeng, Zhang Wan, Wang Zhongfu, Zhang Jianbo. Substoichiometric FeCl₃Activation of Propargyl Glycosides for the Synthesis of Disaccharides andGlycoconjugates. Synlett (2018), 29(5): 668-672.
17. SunYujiao, Gong Guiping, Guo Yiming, WangZhongfu, Song Shuang, Zhu Beiwei, Zhao Lili, Jiang Jinju. Purification,structural features and immunostimulatory activity of novel polysaccharidesfrom Caulerpa lentillifer. International Journal of BiologicalMacromolecules (2018), 108: 314-323.
18. Shang Jiao, Fang Minfeng, Zhang Li, Wang Hui, Gong Guiping, Wang Zhongfu, Zhao Ajing, Yi Huihui. Purification and activitycharacterization of polysaccharides in the medicinal lichen Umbilicaria tornatafrom Taibai Mountain, China. Glycoconjugate Journal (2018), 35(1):107-117.
19. SongShuang, Zhang Bao, Wu Sufeng, Huang Lu, Ai Chunqing, Pan Jinfeng, Su Yi-Cheng, Wang Zhongfu, Wen Chengrong. Structuralcharacterization and osteogenic bioactivity of a sulfated polysaccharide frompacific abalone (Haliotis discus hannai Ino). Carbohydrate Polymers(2018), 182: 207-214.
20. WeiJinhua, Wang Zhuo A, Wang Bing, Jahan Marefa, Wang Zhongfu, Du Yuguang, Wynn Peter C. Characterization of porcinemilk oligosaccharides over lactation between primiparous and multiparous femalepigs. Scientific reports (2018), 8(1): 4688.
21. WangJianhui, Cheng Xiaorui, Zeng Ju, Zhou Wenxia, Zhang Yongxiang, Yuan Jiangbei, Wang Zhongfu. LW-AFC Effects onN-glycan Profile in Senescence-Accelerated Mouse Prone 8 Strain, a Mouse Modelof Alzheimer's Disease. Aging and disease (2017),8(1):101-114.
22. TanShuguang, Zhang Hao, Chai Yan, Song Hao, Tong Zhou, Wang Qihui, Qi Jianxun,Wong Gary, Zhu Xiaodong, Liu William J, Gao Shan, Wang Zhongfu, Shi Yi, Yang Fuquan, Gao George F, Yan Jinghua. Anunexpected N-terminal loop in PD-1 dominates binding by nivolumab. NatureCommunications (2017), 8: 14369.
23. WangChengjian, Zhang Ping, Jin Wanjun, Li Lingmei, Qiang Shan, Zhang Ying, HuangLinjuan*, Wang Zhongfu*.Quantitative O-glycomics based on improvement of the one-pot method fornonreductive O-glycan release and simultaneous stable isotope labeling with1-(d0/d5)phenyl-3-methyl-5-pyrazolone followed by mass spectrometric analysis. Journalof Proteomics (2017), 150: 18-30.
24. SunGuosheng, Qiu Saifeng, Ding Zekun, Chen Heshan, Zhou Jiafen, Wang Zhongfu, Zhang Jianbo. MagneticCore-Shell Fe₃O₄@C-SO₃H as an Efficient andRenewable 'Green Catalyst' for the Synthesis of O-2, 3-UnsaturatedGlycopyranosides. Synlett (2017), 28(3), 347-352.
25. ZhangWan, Luo Xiaosheng, Wang Zhongfu,Zhang Jianbo. One-pot synthesis of β-2,6-dideoxyglycosides via glycosyl iodideintermediates. Journal of Carbohydrate Chemistry (2016), 35(6): 315-325.
26. PengYifang, Wang Chengjian, Wang Jingjing, Li Lingmei, Jin Wanjun, Qiang Shan, ShiHongdan, Zhang Ying, Huang Linjuan, WangZhongfu*. Analysis of antigenic determinant glycans of peanut allergyglycoprotein Ara h1 by mass spectrometry. Gaodeng Xuexiao Huaxue Xuebao(2016), 37(9): 1622-1628.
27. QiuSaifeng, Zhang Wan, Sun Guosheng, WangZhongfu, Zhang Jianbo. A Facile and Direct Glycosidation Method for theSynthesis of 2-Deoxy α-Rhamnosides Catalyzed by Ferric Chloride. ChemistrySelect (2016), 1(15): 4840-4844.
28. LiLingmei, Wang Chengjian, Qiang Shan, Zhao Jixiang, Song Shuang, Jin Wanjun,Wang Bo, Zhang Ying, Huang Linjuan*, WangZhongfu*. Mass Spectrometric Analysis of N-Glycoforms of Soybean AllergenicGlycoproteins Separated by SDS-PAGE. Journal of Agricultural and Food Chemistry (2016),64(39): 7367-7376.
29. ChenZhina, Shi Junling, Yang Xijuan, Liu Yang, Nan Bo, Wang Zhongfu. Isolation of exopolysaccharide-producing bacteria andyeasts from Tibetan kefir and characterisation of the exopolysaccharides.International Journal of Dairy Technology (2016), 69(3): 410-417.
30. ChenHeshan, Xian Ting, Zhang Wan, Si Wenshuai, Luo Xiaosheng, Zhang Bo, ZhangMeiyu, Wang Zhongfu, Zhang Jianbo.An efficient method for the synthesis of pyranoid glycals. Carbohydrate Research(2016), 431: 42-46.
31. BaiLiang, Li Qianwei, Li Lingmei, Lin Yan, Zhao Sihai, Wang Weirong, Wang Rong, LiYongqin, Yuan Jiangbei, Wang Chengjian, WangZhongfu*, Fan Jianglin, Liu Enqi*. Plasma high-mannose and complex/hybridN-glycans are associated with hypercholesterolemia in humans and rabbits. PLoSOne (2016), 11(3): e**/1 -e**/16.
32. LiuYang, Yin Lu, Gong Guiping, Peng Yifang, Huang Linjuan, Wang Zhongfu*. Structural characterization, antioxidant activityand immunomodulatory activity of the polysaccharide LRLP3 from leaves of Lyciumruthenicum murra. Gaodeng Xuexiao Huaxue Xuebao (2016), 37(2): 261-268.
33. LiuYang, Gong Guiping, Sun Yujiao, Gu Xiao, Huang Linjuan, Wang Zhongfu*. Isolation, structural characterization, andimmunological activity of a polysaccharide LRLP4-A from the leaves of Lyciumruthenicum. Journal of Carbohydrate Chemistry (2016), 35(1), 40-56.
34. GongGuiping, Fan Jiangbo, Sun Yujiao, Wu Yanmin, Liu Yang, Sun Wei, Zhang Ying*, Wang Zhongfu*. Isolation, structuralcharacterization, and antioxidativity of polysaccharide LBLP5-A from Lyciumbarbarum leaves. Process Biochemistry (2016), 51(2): 314-324.
35. Zhang Ying，LiuYing，LiuYang，QiangShan，YangBingying，HuangLinjuan，Wang Zhongfu*.Pretreatment Method for Detecting of N-linked Glycans of Glycoprotein inMicro-scale Complex Biological Sample based on Electrospray Ionization MassSpectrometry. Chinese Journal of Analytical Chemistry (2016), 44(2):265-272.
36. LuHua, Lu Yang, Ren Jinwei, Wang Zhongfu,Wang Qian, Luo Yuanming, Han Jing, Xiang Hua, Du Yuguo, Jin Cheng.Identification of the S-layer glycoproteins and their covalently linked glycansin the halophilic archaeon Haloarcula hispanica. Glycobiology (2015),25(11), 1150-1162.
37. PanLiying, Gu Xiao, Wang Chengjian, Qiang Shan, Huang Linjuan, Zhang Ying*, Wang Zhongfu*. Comparative analysis ofO-glycans from human hepatocellular carcinoma HepG2 and normal liver cells L02.Gaodeng Xuexiao Huaxue Xuebao (2015), 7: 1275-1281.
38. LiWenzhe, Yu Rui, Ma Biao, Yang Yan, Jiao Xinyan, Liu Yang, Cao Hongyu, DongWeijie, Liu Linhua, Ma Keli, Fukuda Tomohiko, Liu Qingping, Ma Tonghui, Wang Zhongfu, Gu Jiangguo, ZhangJianing, Taniguchi Naoyuki. Core Fucosylation of IgG B Cell Receptor IsRequired for Antigen Recognition and Antibody Production. Journal of Immunology (2015),194(6): 2596-2606.
39. SunYujiao, Yang Bingying, Wu Yanmin, Liu Yang, Gu Xiao, Zhang Hong, WangChengjian, Cao Hongzhi, Huang Linjuan*, WangZhongfu*. Structural characterization and antioxidant activities ofκ-carrageenan oligosaccharides degraded by different methods. FoodChemistry (2015), 178: 311-318.
40. SunYujiao, Sun Wei, Guo Jiatong, Hu Xiuting, Gong Guiping, Huang Linjuan, CaoHongzhi, Wang Zhongfu*. Sulphationpattern analysis of chemically sulphated polysaccharide LbGp1 from Lyciumbarbarum by GC–MS. Food Chemistry (2015), 170: 22–29.
41. ChenZhina, Shi Junling, Yang Xijuan, Nan Bo, Liu Yang, Wang Zhongfu. Chemical and physical characteristics and antioxidantactivities of the exopolysaccharide produced by Tibetan kefir grains duringmilk fermentation. International Dairy Journal (2015), 43: 15-21.
42. PanLiying, Wang Chengjian, Yuan Jiangbei, Zhang Ying, Huang Linjuan, Wang Zhongfu*. Qualification andquantification for comparative analysis of N-glycans from human hepatocellularcarcinoma HepG2 and normal liver cells L02 by electrospray ionization massspectrometry. Gaodeng Xuexiao Huaxue Xuebao (2014), 35(2): 237-243.
43. YuanJiangbei, Wang Chengjian, Sun Yujiao, HuangLinjuan*, Wang Zhongfu*. Nonreductive chemical release of intact N-glycansfor subsequent labeling and analysis by mass spectrometry. Analytical Biochemistry(2014), 462: 1-9.
44. Zhang Ying, Wang Chengjian, Liu Yang, Yao Weinan, Sun Yujiao, Zhang Ping, HuangLinjuan, Wang Zhongfu*.Fluorescein-5-thiosemicarbazide (FTSC) labeling for fluorescent imaging ofpectin-derived oligogalacturonic acid transported in living cells by confocalmicroscopy. European Food Research and Technology (2014), 239(5): 867-875.
45. Jiang Kuan, Wang Chengjian, Sun Yujiao, Liu Yang, Zhang Ying, Huang Linjuan*, Wang Zhongfu*. Comparison of Chickenand Pheasant Ovotransferrin N-Glycoforms via Electrospray Ionization MassSpectrometry and Liquid Chromatography Coupled with Mass Spectrometry. Journalof Agricultural and Food Chemistry (2014), 62(29): 7245-7254.
46. XueXiangdong, Sun Yujiao, Liu Yang, Zhang Ping, Wang Zhongfu, Huang Linjuan. A novel method for relativequantitation of N-glycans via acetone stable isotopic labeling and ESI-MSanalysis. Huaxue Xuebao (2014), 72(2): 220-226.
47. Wang Zhongfu,Liu Yang, Sun Yujiao, Mou Qing, Wang Bo, Zhang Ying, Huang Linjuan. Structuralcharacterization of LbGp1 from the fruits of Lycium barbarum L. FoodChemistry (2014), 159: 137-142.
48. SunYujiao, Liu Yang, Jiang Kuan, Wang Chengjian, Wang Zhongfu, Huang Linjuan. Electrospray Ionization MassSpectrometric Analysis of κ-Carrageenan Oligosaccharides Obtained byDegradation with κ-Carrageenase from Pedobacter hainanensis. Journalof Agricultural and Food Chemistry (2014), 62(11), 2398-2405.
49. WangChengjian, Wu Zhiyu, Yuan Jiangbei, Wang Bo, Zhang Ping, Zhang Ying, Wang Zhongfu*, Huang Linjuan*.Simplified Quantitative Glycomics Using the Stable Isotope Label Girard'sReagent P by Electrospray Ionization Mass Spectrometry. Journal of Proteome Research (2014), 13(2): 372-384.
50. Zhang Hong, Wang Hai-Feng, Liu Yang, Huang Linjuan, Wang Zhongfu*, Li Ye. The haematopoietic effect of Panax japonicuson blood deficiency model mice. Journal of Ethnopharmacology (2014),154: 818–824.
51. Zhang Jianbo, Zhang Bo, Zhou Jiafen, Chen Heshan, Li Juan, Yang Guofang, Wang Zhongfu, Tang Jie. A Facile H₂SO₄-SiO₂-CatalyzedFerrier Rearrangement of 3,4,6-Tri-O-benzyl-d-glucal. Journal of CarbohydrateChemistry (2013), 32(5-6): 380-391.
52. WangChengjian, Yuan Jiangbei, Li Xiaohua, WangZhongfu*, Huang Linjuan*. Sulfonyl hydrazine-functionalized polymer as aspecific capturer of reducing glycans from complex samples for high-throughputanalysis by electrospray ionization mass spectrometry. Analyst (2013), 138(18):5344-5356.
53. Zhang Ying, Yuan Jiangbei, Song Jingjing, WangZhongfu*, Huang Linjuan*. An efficient method for selectively imaging andquantifying in situ the expression of sialylated glycoproteins on living cells.Glycobiology(2013), 23(6): 643-653.
54. LiQian, Zhou Siyuan, Jing Juan, Yang Tiehong, Duan Shuangyan, Wang Zhongfu, Mei Qibing, Liu Li.Oligosaccharide from apple induces apoptosis and cell cycle arrest in HT29human colon cancer cells. International Journal of BiologicalMacromolecules (2013), 57: 245-254.
55. WangChengjian, Yuan Jiangbei, Wang Zhongfu*,Huang Linjuan*. Separation of one-pot procedure released O-glycans as1-phenyl-3-methyl-5-pyrazolone derivatives by hydrophilic interaction andreversed-phase liquid chromatography followed by identification usingelectrospray mass spectrometry and tandem mass spectrometry. Journalof Chromatography A (2013), 1274: 107-117.
56. LvXiaopeng, Wang Chengjian, Cheng Yang, Huang Linjuan*, Wang Zhongfu*. Isolation and structural characterization of apolysaccharide LRP4-A from Lycium ruthenicum Murr. Carbohydrate Research(2013), 365: 20-25.
57. PengQiang, Song Jingjing, Lv Xiaopeng, WangZhongfu, Huang Linjuan, Du Yuguang. Structural Characterization of anArabinogalactan-Protein from the Fruits of Lycium ruthenicum. Journalof Agricultural and Food Chemistry (2012), 60(37): 9424-9429.
58. Zhang Ying, Sun Yujiao, Wang Zhongfu*,Huang Linjuan*. Fluorescein-5-thiosemicarbazide as a probe for directly imagingof mucin-type O-linked glycoprotein within living cells. Glycoconjugate Journal (2012), 29(5-6): 445-452.
59. Zhang Ying, Liu Yang Wang Zhijun, Wang Zhongfu,Huang Linjuan. The synthesis of a 2-deoxy-2-acetonyl sugar from itscorresponding natural saccharide. Journal of Chemical Research (2012),36(4): 244-246.
60. WangChengjian, Fan Wancui, Zhang Ping, WangZhongfu*, Huang Linjuan*. One-pot nonreductive O-glycan release andlabeling with 1-phenyl-3-methyl-5-pyrazolone followed by ESI-MS analysis. Proteomics (2011), 11(21), 4229-4242.
61. Zhang Jianbo, Zhang Bo, Zhou Jiafen, Li Juan, Shi Chunjuan, Huang Ting, Wang Zhongfu, Tang Jie. H₂SO₄-SiO₂:Highly Efficient and Reusable Catalyst for per-O-Acetylation of CarbohydratesUnder Solvent-Free Conditions. Journal of Carbohydrate Chemistry (2011), 30(3): 165-177.
62. Zhang Ying, Zhang Ping, Wang Zhongfu,Huang Linjuan. An Innovative Derivatization Method for SimultaneousDetermination of Uronic Acids and Neutral and Amino Sugars in CoexistingSamples by HPLC-ESI-MS/MS². Journal of Liquid Chromatography &Related Technologies (2011), 34(16):1754-1771.
63. Zhang Jianbo, Fu Jie, Si Wenshuai, Wang Xiaohu, WangZhongfu*, Tang Jie. A highly efficient deprotection of the2,2,2-trichloroethyl group at the anomeric oxygen of carbohydrates.Carbohydrate Research (2011), 346(14): 2290-2293.
64. 获奖情况 1.《寡糖/多糖分析鉴定方法和技术体系的建立及应用》，陕西高等学校科学技术奖，一等奖，王仲孚（1/11）。（2017）
2. 入选陕西省高层次人才特殊支持计划科技创新领军人才。（2017）
3. 入选陕西省创新人才推进计划“中青年科技创新领军人才”(2016)
4. 首届“张树政糖科学奖?优秀青年奖”获得者（2015）