教育背景
博士 起止时间：1990-1993，毕业院校：山东大学，专业：微生物学
硕士 起止时间：1987-1990，毕业院校：山东农业大学，专业：真菌学
学士 起止时间：1983-1987，毕业院校：山东大学，专业：微生物学



工作经历
起止时间 单位名称 专业技术职务
1993-1995 中科院沈阳应用生态所 博士后
1995-2000 山东大学微生物技术国家重点实验室 副教授
1996.4-1996.10 德国国家生物技术研究中心（GBF） 高级访问****
2000-今 山东大学微生物技术国家重点实验室 教授

研究方向
粘细菌（Myxobacteria）在系统进化上属于Proteobacteria 类群的δ 分支。在其生活史中表现复杂的多细胞群体行为特性，显示与多细胞真核生物较多的相似性，显著不同于其他细菌，被认为是生活于多细胞生命边缘的单细胞生物类群，是一类‘高等’的原核生物，是细菌细胞间通讯与协作，多细胞形态发生，以及生命进化等研究的重要模式生物材料。粘细菌能够降解利用其他活的微生物细胞、细胞残体以及许多难降解的大分子有机物，是一类捕食性细菌，在微生物生态系统扮演重要角色。粘细菌拥有庞大的基因组，是目前报道的原核生物中最大的。粘细菌能够产生丰富的次级代谢产物，具有种类多、结构新、作用机理新颖多样的特性，被公认是继放线菌之后又一个重要的药源微生物新类群。
我们以粘细菌为材料，开展如下方面的研究：
1、粘细菌多样性及其生态功能
2、粘细菌基因组中重要基因的重复及其功能机制
3、粘细菌产生的抗肿瘤化合物埃博霉素及其生物合成改造
代表性论文
1、 XJ Yue, XW Cui, Z Zhang, WF Hu, ZF Li, YM Zhang and YZ Li*. Effects of transcriptional mode on promoter substitution and tandem engineering for the production of epothilones in Myxococcus xanthus. Appl Microbiol Biotechnol. (2018) doi.org/10.1007/s00253-018-9023-4
2、 R Peng, Y Wang, WW Feng, XJ Yue, JH Chen, XZ Hu, ZF Li, DH Sheng, YM Zhang and YZ Li*. CRISPR/dCas9?mediated transcriptional improvement of the biosynthetic gene cluster for the epothilone production in Myxococcus xanthus. Microb Cell Fact (2018) 17:15.
3、 L Zhuo, Y Wang, Z Zhang, J Li, XH Zhang, YZ Li*. Myxococcus xanthus DK1622 coordinates expressions of the duplicate groEL and single groES genes for synergistic functions of GroELs and GroES. Frontiers in Microbiology (2017) 8: 733.
4、 R Peng, JH Chen, WW Feng, Z Zhang, J Yin, ZS Li, YZ Li*. Error-prone DnaE2 balances the genome mutation rates in Myxococcus xanthus DK1622. Frontiers in Microbiology (2017) 8: 122.
5、 XJ Yue, XW Cui, Z Zhang, R Peng, P Zhang, ZF Li, YZ Li*. A bacterial negative transcription regulator binding on an inverted repeat in the promoter for epothilone biosynthesis. Microbial Cell Factories (2017) 16: 92.
6、 YJ Yang, Y Wang, ZF Li, Y Gong, P Zhang, WC Hu, DH Sheng and YZ Li*. Increasing on target cleavage efficiency for CRISPR/Cas9 induced large fragment deletion in Myxococcus Xanthus. Microb Cell Fact (2017) 16:142.
7、 XJ Chen, K Han, J Feng, YZ Li*. The complete genome sequence and analysis of a plasmid-bearing myxobacterial strain Myxococcus fulvus 124B02. Standards in Genomic Sciences. (2016)11:1.
8、 LP Zhu, XJ Yue, K Han, ZF Li, LS Zheng, XN Yi, HL Wang, YM Zhang and YZ Li*. Allopatric integrations selectively change host transcriptomes, leading to varied expression efficiencies of exotic genes in Myxococcus xanthus. Microb Cell Fact (2015) 14:105.
9、 Y Wang, YZ Li*. Cultivation to improve in vivo solubility of overexpressed arginine deiminases in Escherichia coli and the enzyme characteristics. BMC biotechnology (2014) 14: 53.
10、 Y Wang, X Li, W Zhang, X Zhou, YZ Li*. groEL2, but not groEL1, is required for the biosynthesis of the secondary metabolite myxovirescin in Myxococcus xanthus DK1622. Microbiology (2014) 160, 488-495.
11、 SG Li, L Zhao, K Han, PF Li, ZF Li, H Liu, ZH Wu, YZ Li*. Diversity of epothilone producers among Sorangium strains in producer-positive soil habitats. Microb Biotechnol (2014) 7(2), 130-141.
12、 XW Zhou, SG Li, W Li, DM Jiang, K Han, ZH Wu and YZ Li*. Myxobacterial community is a predominant and highly diverse bacterial group in soil niches. Env Microbiol Rep (2014) 6(1):45-56.
13、 PY Zhang, PP Xu, ZJ Xia, J Wang, J Xiong and YZ Li*. Combined treatment with the antibiotics kanamycin and streptomycin promotes the conjugation of Escherichia coli. FEMS Microbiol Lett (2014) 348: 149–156.
14、 K Han, ZF Li, R Peng, LP Zhu, T Zhou, LG Wang, SG Li, XB Zhang, W Hu, ZH Wu, N Qin and YZ Li*. Extraordinary expansion of a Sorangium cellulosum genome from an alkaline milieu. Sci Rep (2013) 3, 2101.
15、 PF Li, SG Li, ZF Li, L Zhao, T Wang, HW Pan, H Liu, ZH Wu and YZ Li*. Co-cultivation of Sorangium cellulosum strains affects cellular growth and biosynthesis of secondary metabolite epothilones. FEMS Microbiol Ecol (2013) 85, 358-368.
16、 WJ Han, JY Gu, HH Liu, FC Li, ZH Wu, YZ Li*. An extra peptide within the catalytic module of a beta-agarase affects the agarose degradation pattern. J Biol Chem (2013) 288(13): 9519–9531.
17、 Y Wang, WY Zhang, Z Zhang, J Li, ZF Li, ZG Tan, TT Zhang, ZH Wu, H Liu, YZ Li*. Mechanisms involved in the functional divergence of duplicated GroEL chaperonins in Myxococcus xanthus DK1622. PLoS Genet (2013) 9(2): e**.
18、 J Feng, XJ Chen, X Sun, N Wang, YZ Li*: Characterization of the replication origin of the myxobacterial self-replicative plasmid pMF1. Plasmid (2012) 68: 105–112.
19、 SG Li, XW Zhou, PF Li, K Han, W Li, ZF Li, ZH Wu, YZ Li*: The existence and diversity of myxobacteria in lake mud-a previously unexplored myxobacteria habitat. Environ Microbiol Rep (2012) 4(6): 587-595.
20、 YY Cheng, YK Qian, ZF Li, ZH Wu, H Liu and YZ Li*: A novel cold-adapted lipase from Sorangium cellulosum strain So0157-2: gene cloning, expression, and enzymatic characterization. Int J Mol Sci (2011) 12: 6765-6780.
21、 ZF Li, X Li, H Liu, X Liu, K Han, ZH Wu, W Hu, FF Li, YZ Li*: Genome Sequence of the Halotolerant Marine Bacterium Myxococcus fulvus HW-1, J Bacteriol (2011) 193: 5015-5016.
22、 X Sun, XJ Chen, J Feng, JY Zhao, YZ Li*: Characterization of the Partitioning System of Myxococcus Plasmid pMF1. PLoS ONE (2011) 6，e28122.
23、 DM Jiang, C Kato, XW Zhou, ZH Wu, T Sato, YZ Li*: Phylogeographic separation of marine and soil myxobacteria at high levels of classification. The ISME Journal (2010) 4: 1520-1530.
24、 HW Pan, ZG Tan, H Liu, ZF Li, CY Zhang, CY Li, J Li, YZ Li*. Hdsp, a horizontally transferred gene required for social behavior and halotolerance in salt-tolerant Myxococcus fulvus HW-1. The ISME Journal (2010) 4: 1282-1289.
25、 CH Lu, XL Liu, YZ Li*, YM Shen*. Two 18-membered epothilones from Sorangium cellulosum So0157-2. J Antibiot (2010) 63: 571-574.
26、 L Zhao, PF Li, CH Lu, SG Li, YM Shen*, YZ Li*: Glycosylation and production characteristics of epothilones in alkali-tolerant Sorangium cellulosum strain So0157-2. J Microbiol (2010) 48 (4): 438-444.
27、 J Li, Y Wang, CY Zhang, WY Zhang, DM Jiang, ZH Wu, H Liu, YZ Li*. Myxococcus xanthus viability depends on GroEL supplied by either of two genes, but the paralogs have different functions during heat shock, predation, and development. J Bacteriol (2010) 192(7): 1875-1881.
28、 HW Pan, H Liu, T Liu, CY Li, ZF Li, K Cai, CY Zhang, Y Zhang, W Hu, ZH Wu, YZ Li*. Seawater-regulated genes of two-component systems and outer membrane proteins in Myxococcus. J Bacteriol (2009) 191(7):2102-2111.
29、 ZJ Xia, J Wang, W Hu, H Liu, XZ Gao, ZH Wu, PY Zhang, YZ Li*. Improving conjugation efficacy of Sorangium cellulosum by the addition of dual selection antibiotics. J Ind Microbiol Biotechnol (2008) 35(10): 1157-1163.
30、 JY Zhao, L Zhong, MJ Shen, ZJ Xia, QX Cheng, X Sun, GP Zhao, YZ Li*, ZJ Qin*. Discovery of an autonomously-replicating plasmid pMF1 from Myxococcus fulvus and the development of a gene cloning system in Myxococcus xanthus. Appl Environ Microbiol (2008) 74(7): 1980-1987.
31、 DM Jiang, L Zhao, CY Zhang, J Li, ZJ Xia, J Wang, ZH Wu, YZ Li*. Taxonomic analysis of Sorangium species based on HSP60 and 16S rRNA gene sequences and morphology. Int J Syst Evol Microbiol (2008) 58(11): 2654-2659.
32、 JY Zhao, ZJ Xia, X Sun, L Zhong, DM Jiang, H Liu, J Wang, ZJ Qin, YZ Li*. Cloning and characterization of an rRNA methyltransferase from Sorangium cellulosum. Biochem Biophys Res Co (2008) 370: 140-144.
33、 CY Zhang, K Cai, H Liu, Y Zhang, HW Pan, B Wang, ZH Wu, W Hu, YZ Li*. A new locus important for Myxococcus social motility and development. J Bacteriol (2007) 189: 7937-7941.
34、 GL Gong, X Sun, XL Liu, W Hu, WR Cao, H Liu, WF Liu, YZ Li*. Mutation and a high-throughput screening method for improving the production of Epothilones of Sorangium. J Ind Microbiol Biotechnol (2007) 34(9): 615-623.
35、 DM Jiang, ZH Wu, JY Zhao, YZ Li*. Fruiting and non-fruiting myxobacteria: a phylogenetic perspective of cultured and uncultured members of this group. Mol Phylogenet Evol (2007) 44(2):545-552.
36、 B Wang, W Hu, H Liu, CY Zhang, JY Zhao, DM Jiang, ZH Wu, YZ Li*. Adaptation of salt-tolerant Myxococcus strains and their motility systems to the ocean conditions. Microb Ecol (2007) 54(1): 43-51.
37、 ZF Li, JY Zhao, ZJ Xia, J Shi, H Liu, ZH Wu, W Hu, WF Liu, YZ Li*.Evolutionary diversity of ketoacyl synthases in cellulolytic myxobacterium Sorangium. Syst Appl Microbiol (2007) 30: 189-196.
38、 H Zhang, J Du, J Zhao, YZ Li*: Evaluation of fusion between liposomes and erythrocytes with capillary electrophoresis and laser-induced fluorescence detection. J Chromatogr A (2006) 1110: 81-85.
39、 PB Hou, YZ Li*, BH Wu, ZC Yan, BX Yan, PJ Gao. Cellulolytic Complex exists in Cellulolytic Myxobacterium Sorangium. Enzyme Microbiol Technol (2006) 38: 273–278.
40、 ZH Wu, DM Jiang, P Li, YZ Li*. Exploring diversity of myxobacteria in a soil niche by myxobacteria-specific primers and probes. Environ Microbiol (2005) 7(10): 1602-1610.
41、 YQ Zhang, YZ Li*, B Wang, ZZ Wu, CZ Zhang, X Gong, ZJ Qiu, Y Zhong. Characteristics and living pattern of marine myxobacterial isolates. Appl Environ Microbiol (2005) 71(6): 3331–3336．
42、 H Zhang, H Dong, J Zhao, W Hu, YZ Li*. Characterization of developmental autolysis in myxobacterial fruiting body morphogenesis with profiling of amino acids using capillary electrophoresis method. Amino Acids (2005) 28: 319-325．
43、 H Dong, YZ Li*, W Hu. Analysis of purified tubulin in high concentration of glutamate for application in high throughput screening for microtubule-stabilizing agents. Assay Drug Dev Technol (2004) 2(6): 621-628.
44、 W Hu, H Dong, YZ Li*, XT Hu, GJ Han, YB Qu. A high-throughput model for screening anti-tumor agents capable of promoting polymerization of tubulin in vitro. Acta Pharmacol Sin (2004) 25 (6): 775-782.
45、 E Nguimbi, YZ Li*, BL Gao, ZF Li, B Wang, ZH Wu, BX Yan, YB Qu, PJ Gao. 16S-23S Ribosomal DNA Intergenic Spacer Regions in Cellulolytic Myxobacteria and Differentiation of Closely Related Strains. Syst Appl Microbiol (2003) 26(2): 262-268.
46、 ZC Yan, B Wang, YZ Li*, X Gong, HQ Zhang, PJ Gao. Morphologies and phylogenetic classification of cellulolytic myxobacteria. Syst Appl Microbiol (2003) 26(1): 104-108.
47、 Li YZ*, Hu W, Zhang YQ, Qiu ZJ, Zhang Y, Wu BH: A simple method to isolate salt-tolerant myxobacteria from marine samples. J Microbiol Meth (2002) 50: 205-209.


获奖情况


1、粘细菌资源多样性的应用基础研究，自然科学奖一等奖，教育部（第一位），2017
2、粘细菌产生的抗肿瘤化合物的应用与研究，技术发明二等奖，山东省（第一位），2007