王瑞旋¹, 王江勇², 李韵萍³, 郭子晗⁴, 李炳², 李云¹, 朱慧¹
1.韩山师范学院, 广东 潮州 521041;2.中国水产科学研究院南海水产研究所, 广东 广州 510300;3.华南师范大学, 广东广州 510631;4.加尼福利亚浸会大学, 美国 加利福尼亚 92504

摘要:
随着抗菌药物在水产养殖中用量的增加及混合使用现象的出现，水产病原菌表现出更强的耐药性甚至是多重耐药性，大大增加了水产养殖中细菌性病害防治的难度。细菌耐药性最常见的传递方式是耐药质粒的转移。本研究通过对多个水产致病弧菌质粒上新霉素及卡那霉素的耐药基因aph（3'）-IIa进行检测分析，并利用电穿孔法将筛选得到的3个受试菌株上的质粒分别转入大肠杆菌中，结果从3个转化子中检测到目的基因。进一步的药敏测试结果显示，3个转化子的最小抑菌浓度（MIC值）以及最小杀菌浓度（MBC值）均明显上升。可见，在特定条件下，水生致病性弧菌对氨基糖苷类（新霉素和卡那霉素）的耐药质粒可转移至大肠杆菌并参与介导其耐药性，大大降低了受体菌对新霉素和卡那霉素的敏感性。本研究将为环境弧菌与临床细菌对氨基糖苷类药物的抗性传递研究提供数据基础和参考依据。
关键词:致病弧菌耐药质粒新霉素卡那霉素耐药机制
DOI：10.11759/hykx20191221002
分类号:Q931
基金项目:广东省自然科学基金项目（2017A030313112）；国家自然科学基金项目（31902416）；广东省现代农业产业技术体系岗位专家（粤财农[2019]73号）；国家贝类产业技术体系岗位专家（CARS-49）；广东省教育厅特色创新科研项目（2016KTSCX086）

Primary research on the transmission mechanism of resistance to aminoglycoside for pathogenic marine Vibrio strains
WANG Rui-xuan¹, WANG Jiang-yong², LI Yun-ping³, GUO Zi-han⁴, LI Bing², LI Yun¹, ZHU Hui¹
1.Hanshan Normal University, Chaozhou 521041, China;2.South China Sea Fisheries Research Institute, Guangzhou 510300, China;3.South China Normal University, Guangzhou 510631, China;4.California Baptist University, California 92504, USA
Abstract:
The growing number of antimicrobial agents in aquaculture and the development of multi-antibiotics use have led to significant antibiotic-resistances and multi-resistance, which has increased the prevention and control of bacterial diseases in aquaculture. The most effective way for bacteria to spread antibiotic resistance is through the transfer of resistant plasmids. In the present study, the aph(3')-IIa aminoglycoside-resistant gene located on plasmid DNA was amplified by polymerase chain reaction and then three resistant plasmids were sequenced and transformed into Escherichia coli by electroporation. It has been shown that the target gene sequence of three mutants was detected. The result of resistance analysis suggested that the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) of three transformants were all much higher than the MIC and the MBC of standard strain E. coli. It suggested that drug-resistant plasmids in vibrio spp. could be transferred to E. coli, and the resistant plasmid had participated in the resistance mechanism to control the resistance of strains to aminoglycosides, such as neomycin and kanamycin. This study will provide important data and be a reference for the study on the transmission of resistance to aminoglycoside between environmental vibrios and clinical bacteria.
Key words:pathogenic vibrioantibiotic-resistant plasmidneomycinkanamycindrug resistance mechanism





PDF全文下载地址:

点我下载PDF