王蔚芳¹, 向玲², 王琳¹, 黄滨¹, 李会涛³
1.中国水产科学研究院黄海水产研究所, 青岛海洋科学与技术试点国家实验室 海洋渔业科学与食物产出过程功能实验室, 山东 青岛 266071;2.中国海洋大学水产学院, 山东 青岛 266003;3.山东百福生物科技有限公司, 山东 济宁 273200

摘要:
本实验旨在探究维生素E对半滑舌鳎垂体组织中生长激素基因表达的影响。作者在每千克等氮等能的基础饲料中分别添加0、400和1 600 mg的DL-α-生育酚乙酸酯（维生素E）投喂半滑舌鳎（Cynoglossus semilaevis）成鱼（464±2.6）g，进行为期8周的养殖实验；另外，在L-15培养基中添加0、18和54 μmol/L的维生素E，对半滑舌鳎成鱼（464±2.6）g的垂体细胞进行为期3 d的体外原代培养实验。分别取垂体组织和原代细胞，通过荧光实时定量PCR分析其gh mRNA的相对表达量。实验结果表明：垂体组织中gh mRNA的相对表达量随着饲料中维生素E含量的增加而呈现先升高后下降的变化趋势，在400 mg/kg组时显著高于其他各组（P<0.05）；随着细胞培养液中维生素E浓度的升高，gh mRNA的相对表达量显著增加（P<0.05）。由此可见，适宜浓度的维生素E能够促进半滑舌鳎垂体组织中生长激素基因的表达。
关键词:半滑舌鳎(Cynoglossus semilaevis)维生素E生长激素基因养殖垂体原代细胞
DOI：10.11759/hykx20190802001
分类号:S968.9
基金项目:国家自然科学基金面上项目（31872578）；国家自然科学基金青年项目（31502177）

An appropriate concentration of vitamin E promotes gene expression of growth hormone in Cynoglossus semilaevis
WANG Wei-fang¹, XIANG Ling², WANG Lin¹, HUANG Bin¹, LI Hui-tao³
1.Laboratory for Marine Fisheries Science and Food Production Processes, Pilot National Laboratory for Marine Science and Technology of Qingdao, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;2.College of Fisheries, Ocean University of China, Qingdao 266003, China;3.Shandong Baifu Biotech Co., Ltd., Jining 273200, China
Abstract:
This study aimed to investigate the effect of vitamin E on expression of the growth hormone gene gh in pituitary tissues of half-smooth tongue sole (Cynoglossus semilaevis). DL-alpha-tocopherol acetate was added to the basic diet (vitamin E) at concentrations of 0, 400, and 1600 mg/kg diet to feed (464±2.6) g semi-smooth tongue sole for 8 weeks. In addition, 0, 18, and 54 μmol/L vitamin E were added to L-15 medium for primary pituitary cell culture for 3 d. The expression of gh in pituitary tissues and primary pituitary cells was analyzed by real-time quantitative PCR. The results showed that in the in vivo experiment, gh mRNA expression significantly (P<0.05) increased when dietary vitamin E increased from 0 to 400 mg/kg and then significantly decreased in the group that received 1600 mg/kg (P<0.05). In the in vitro experiment, the expression level of gh increased significantly with increasing vitamin E concentration (P<0.05). Therefore, the proper concentration of vitamin E could promote the expression of gh in the pituitary tissue of half-smooth tongue sole.
Key words:Cynoglossus semilaevisvitamin Egrowth hormone geneaquacultureprimary pituitary cell





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