彭长庚 研究员
硕士招生类型：学术型
硕士招生专业：基础医学
博士招生类型：学术型
博士招生专业：临床医学（基础医学方向）
所在院系：医学院
联系方式：Changgeng.peng@@tongji.edu.cn
课题组链接：http://202.120.165.73/ktz/Group.aspx?id=46
研究方向
主要任职
科研业绩


我课题组主要研究方向之一是利用分子生物学、生物信息学和电生理等技术在转基因小鼠模型、疼痛小鼠模型和原代培养的背根节神经元上研究背根神经节感觉神经元的功能和神经病理性疼痛的分子机制，并筛选抑制疼痛的药物。此外，由于大部分帕金森病人遭受慢性疼痛，我课题组有意解析帕金森病与慢性疼痛共发的基因调节网络。
Oneof the main areas of research in my laboratory is to investigate the functionof sensory neurons in dorsal root ganglia (DRG) and the molecular mechanism behindneuropathic pain on models of transgenic mouse and neuropathic pain surgerymouse, as well as primary DRG neuronal culture with technologies of molecularbiology, bioinformatics and electrophysiology, and to screen pain inhibitors.Additionally, my lab is also interested in deciphering the gene regulatorynetwork for the coincidence of Parkinson’s disease and pain because mostpatients of Parkinson’s disease experience chronic pain.







工作和学习经历：
2017.9-至今 特聘研究员、硕士研究生导师、博士研究生导师，同济大学医学院、转化医学高等研究院。
2012.6-2017.6 博士后研究员，瑞典卡罗林斯卡医学院医学生化与生物物理系。
2008.2- 2012.5 博士，德国国家环境与健康研究中心/慕尼黑工业大学，生物学。
2006.7-2007.9 研究助理，中科院上海生命科学研究院健康科学研究所。
2003.9-2006.6 硕士，中科院上海生命科学研究院健康科学研究所，分子生物与生物化学专业。
1999.9-2003.6 学士，山西大学，生物技术专业。
Education and Position:
2017.9-Present PrinciplalInvestigator, Advanced institute of translational medicine, School of Medicine,Tongji University.
2012.6-2017.6 Postdoctoralresearcher, Department of Medical Biochemistry and Biophysics, KarolinskaInstitute.
2008.2- 2012.5 Ph.D., Institute ofDevelopmental Genetics, Helmholtz Zentrum Munich / Technical University Munich,Germany
2006.7-2007.9 ResearchAssistant, Neurogenetics Lab, Shanghai institutes for Biological Sciences,Chinese Academy of Sciences.
2003.9-2006.6 M.S., Shanghaiinstitutes for Biological Sciences, Chinese Academy ofSciences.
1999.9-2003.6 B.S., ShanxiUniversity.






我们最近的研究工作揭示miR-183家族在发育中通过调控SHOX2的表达窗口来控制两类机械刺激感受神经元的比例，在成年时系统地调控神经病理性疼痛基因网络中的核心转录因子网络和包括镇痛药物Gabapentin的靶点CACNA2D1/2在内的80%的基因，来调控神经病理性机械疼痛；并发现神经病理性疼痛的意外机制，即正常不参与疼痛传导的TrkB阳性的低阈值机械感受神经元在神经病理条件下被招募至疼痛传导环路。疼痛研究专家Prof. Barde对我们的miR-183调控神经病理性疼痛的研究工作写了展望评论，发表在Science同期杂志上。

关于中脑多巴胺神经元发育和存活的科研业绩包括：1）发现miR-200家族通过调控SOX2和E2F3来调控中脑神经干细胞的增殖以及miR-200家族通过调控Zeb2影响中脑多巴胺神经元在发育中的迁移；2）发现GDNF-Pitx3-BDNF基因调节通路促进中脑多巴胺神经元的发育和存活；3）证明神经营养因子GDNF对蛋白酶体损害诱导的中脑多巴胺神经元的退化有保护作用。

Our recent research work discovered thatmiR-183 cluster regulates thepopulation sizes of two low threshold mechanoreceptors (LTMRs) throughterminating the expression window of SHOX2 during dorsal root ganglion developmentand scales neuropathic pain in adult by regulating 80% genes in neuropathic paingene network, including a core transcriptional factors network and CACNA2D1/2，the targets of pain inhibitor Gabapentin; and that an unexpectedmechanism behind neuropathic pain, which is that TrkB⁺ LTMRs,normally transmit pleasant tactile sensations, is recruited into nociceptivenetwork under neuropathic condition. Prof. Barde, an expert in pain researchfield, wrote and published a “Perspective Review” about this work on the sameissue of Science.

The contribution to the field ofdevelopment and survival of midbrain dopaminergic neurons (mDA) includes that:1) found that miR-200 family controls proliferation of midbrain stem cellsthrough regulating SOX2 and E2F3 and miR-200 family regulating Zeb2 to affectthe migration of mDA during developing; 2) uncovered that GDNF-Pitx3-BDNF gene regulatory pathwaypromotes the development and survival of midbrain dopaminergic neurons；3）demonstrated that Glialcell-derived neurotrophic factor protects against proteasome inhibition-induceddopamine neuron degeneration.

Publications:
1. SunL^#, Liu Y^# and Peng C. Different Manners ofInterplay between MicroRNAs and Gene Programs in Neuronal Specification. J CellSignal 2018, 3:4. DOI: 10.4172/2576-1471.**.
2. Peng C^1,§， Furlan A, Zhang M, Su J, Lübke M， L?nnerberg P, Abdo H,Sontheimer J, Sundstr?m E, Ernfors P^2§. Termination of cell-type specification gene programsby miR-183 cluster determines the population sizes of low thresholdmechanosensitive neurons. Development. 2018，145, dev165613. doi:10.1242/dev.165613 (§共同通讯作者，1同济大学医学院, 2卡罗琳斯卡医学院).
3.Zhang M, Su J, Adori C, Cinquina V, Malenczyk K, Girach F, Peng C,Ernfors P, L?w P, Borgius L, O, Watanabe M, M, Mitsios N, Mulder J, Harkany T*，H?kfelt T*. Ca2+-bindingprotein NECAB2 facilitates inflammatory pain hypersensitivity. Journal OfClinical Investigation. 2018 July 30. https://doi.org/10.1172/JCI120913.
4.Yang S, Toledo E, Rosmaninho P, Peng C, Uhlén P, Castro D, Arenas E. AZeb2-miR-200c loop controls midbrain dopaminergic neuron neurogenesis andmigration. Communications Biology volume 1, Article number: 75 (2018). https://doi.org/10.1038/s42003-018-0080-0.
5.Peng C, Li L, Zhang M, Gonzales C, Parisien M, Belfer I, Usoskin D, AbdoH, Furlan A, H?ring M, Lallemend F, Harkany T, Diatchenko L, H?kfelt T,Hjerling-Leffler J and Ernfors P. MiR-183 cluster scales mechanical painsensitivity by regulating basal and neuropathic pain genes. Science. 2017Jun 16;356(6343):1168-1171. （Science同期杂志上发表了疼痛研究专家Prof. Barde对我们文章的Perspective review，被F1000推荐为优秀论文）
6.Peng C, Li N, Ng YK, Zhang J, Meier F, Theis FJ, Merkenschlager M, Chen W, Wurst W, Prakash N. Aunilateral negative feedback loop between miR-200 microRNAs and Sox2/E2F3controls neural progenitor cell-cycle exit and differentiation. J Neurosci. 2012Sep 19;32(38):13292-308. （被F1000推荐为优秀论文）
7. Peng C, Aron L,Klein R, Li M, Wurst W, Prakash N, and Le W. Pitx3 is a critical mediator ofGDNF-induced BDNF expression in nigrostriatal dopaminergic neurons. JNeurosci. 2011 Sep;31, 12802-12815.
8. Peng C^*, Fan S^*, Li X, Fan X,Ming M, Sun Z, Le W. Overexpression of pitx3 upregulatesexpression of BDNF and GDNF in SH-SY5Y cells and primary ventral mesencephaliccultures. FEBS Lett. 2007 Apr 3;581(7):1357-61. Epub 2007 Mar2. （co-first author）
9. Li X, Peng C, Li L, Ming M, Yang D, Le W. Glial cell-derived neurotrophic factorprotects against proteasome inhibition-induced dopamine neuron degeneration bysuppression of endoplasmic reticulum stress and caspase-3 activation.J Gerontol A Biol Sci Med Sci. 2007 Sep;62(9):943-50.
10. Yang, D., Peng, C., Li, X., Fan, X., Li, L., Ming,M., Chen, S., and Le, W. Pitx3-transfected astrocytes secrete brain-derivedneurotrophic factor and glial cell line-derived neurotrophic factor and protectdopamine neurons in mesencephalon cultures. J Neurosci Res.2008 Nov; 86(15),3393-3400.
11. Li X, Yang D, Li L, Peng C, Chen S, Le W. Proteasome inhibitor lactacystindisturbs the intracellular calcium homeostasis of dopamine neurons in ventralmesencephalic cultures. Neurochem Int. 2007Jun;50(7-8):959-65. Epub 2007 May 3.

Patent:
1.《Pitx3基因及其启动子的应用》，专利号：6.4，发明人设计人：乐卫东，彭长庚。