摘要/Abstract

过氧化氢（H₂O₂）以分子信号的形式在各种生理和病理过程中都起着重要作用，因此快速、高效、灵敏地检测活细胞或生物体中的H₂O₂具有重要意义.本研究设计了一种新型的H₂O₂比率型荧光探针HDP-1，其对H₂O₂表现出比率荧光响应.探针HDP-1在H₂O₂检测方面具有选择性好、灵敏度高、细胞毒性低和反应速率快等优点，同时，该探针在检测活细胞中外源性和内源性H₂O₂都表现出了优异的性能.
关键词: 比率型荧光探针, 外源, 内源, 过氧化氢, 活细胞成像
Hydrogen peroxide (H₂O₂) plays an important role in various physiological and pathological processes in the form of molecular signals. Thus, it is of great significance to detect hydrogen peroxide in living cells or organisms quickly, efficiently and sensitively. In this study, a novel ratiometric fluorescent probe was designed to show the ratiometric fluorescence response to hydrogen peroxide. The probe showed the advantages of good selectivity, high sensitivity, low cytotoxicity and fast reaction rate in hydrogen peroxide detection. The probe also provided excellent performance in detecting both exogenous and endogenous hydrogen peroxide in living cells.
Key words: ratiometric fluorescent probe, exogenous, endogenous, hydrogen peroxide, living cell imaging


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