摘要/Abstract

报道了两个基于香豆素的荧光探针C1和C2，对次氯酸根均表现出快速的荧光增强响应.尤其是，在化合物C2的溶液中加入次氯酸根后，溶液的荧光发射强度增大了85倍，对次氯酸根的检出限低达1.8×10^-7 mol/L.利用次氯酸根独特的氧化性，化合物C2可实现对次氯酸根的高选择性识别，对其他阴离子及氧化物则几乎无响应.重要的是，化合物C2可穿透细胞膜，用于HeLa细胞中次氯酸根的荧光成像.此外，探针C2还可用于自来水样中次氯酸根的检测.
关键词: 荧光探针, 次氯酸根, 水溶性, 生物成像
Taking advantage of the special oxidation property of hypochlorite, two novel coumarin-type fluorescent probes C1 and C2 were synthesized for ClO^- detection. Both probes could detect ClO^- anions in aqueous solution efficiently with rapid switching-on fluorescent methods. Especially, probe C2 displayed dramatic enhancement in fluorescence emission spectra with the detection limit of 1.8×10^-7 mol/L. In addition to its high selectivity for ClO^- rather than other common anions and reactive oxygen species, C2 was successfully applied to the bioimaging in HeLa cells with ‘turn-on’ fluorescent methods. Moreover, probe C2 could be used for the analysis of ClO^- levels in tap water and potentially in environmental samples.
Key words: fluorescent probe, hypochlorite, good solubility, bioimaging application


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