摘要/Abstract

设计并合成了一种基于菲咯啉的Pb²⁺荧光探针L.在探针L的DMF-H₂O（V：V＝1：1，10 mmol/L Tris，pH＝7.4）溶液中，加入Pb²⁺后导致其荧光猝灭，同时可以观察到溶液的颜色由黄色变为橙色，检测限低至2.68×10^-7 mol/L.通过紫外光谱、荧光光谱、高分辨质谱、核磁共振氢谱和计算拟合等手段研究了传感器L和Pb²⁺离子的络合模式.进一步研究发现，L可以作为一种重复利用的检测Pb²⁺荧光探针，并用于对活细胞中的Pb²⁺的荧光成像.
关键词: 菲咯啉衍生物, Pb²⁺荧光探针, 活细胞成像
A novel fluorescent probe L for Pb²⁺ based phenanthroline derivative has been designed and synthesized. The probe L displayed fluorescence quenching emission excellent sensitivity specific toward Pb²⁺ ion. The introduction of Pb²⁺ resulted the quenching fluorescence of probe L and the color of the solution can be observed changes from yellow to orange in DMF-H₂O (V:V=1:1, 10 mmol/L Tris, pH=7.4). The detection limit was as low as 2.68×10^-7 mol/L. The bonding mechanism was confirmed by UV-vis, fluorescence, HRMS, ¹H NMR experiments and theoretical calculation. It was found that the probe had excellent reversibility for detection of Pb²⁺ and could be used to effectively identify Pb²⁺ in living cells.
Key words: phenanthroline derivative, fluorescent probe for Pb²⁺, living cell imaging


PDF全文下载地址:

点我下载PDF